正交试验法优选清胞衣颗粒水提工艺
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摘要
目的:优选清胞衣颗粒的最佳水提取工艺。方法:以芍药苷、苦杏仁苷的得率以及香蒲新苷与异鼠李素-3-O-新橙皮苷的总得率为评价指标,采用高效液相色谱法测定指标成分含量,通过正交试验设计,采用多指标综合加权评分法,考察加水量、提取次数、提取时间等因素的影响。结果:最佳提取工艺为用12倍量水,浸泡2 h,回流提取2次,每次1 h。结论:优选的提取工艺科学合理,稳定可行,为中试提取提供依据。
Objective: To optimize water reflux extraction of Qing Baoyi Granule. Methods: The extraction technology was optimized by orthogonal text with the yields of paeoniflorin and amygdalin and the total yields of typhaneoside and isorhamnetin- 3-O- neohepeidoside determined by HPLC and multi- index comprehensive weighted scoring method as evaluation index. Results:Optimum extraction technology was as following: soaked 2 h with 12 times the amount of water,extracted 2 times,1 h for each time. Conclusion: The compliance test indicates that the optimized extraction process was scientific,stable and feasible to provide a basis for the extraction of pilot.
Objective: To optimize water reflux extraction of Qing Baoyi Granule. Methods: The extraction technology was optimized by orthogonal text with the yields of paeoniflorin and amygdalin and the total yields of typhaneoside and isorhamnetin- 3-O- neohepeidoside determined by HPLC and multi- index comprehensive weighted scoring method as evaluation index. Results:Optimum extraction technology was as following: soaked 2 h with 12 times the amount of water,extracted 2 times,1 h for each time. Conclusion: The compliance test indicates that the optimized extraction process was scientific,stable and feasible to provide a basis for the extraction of pilot.
引文
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[3]刘成彬,张少聪.中药蒲黄的药理与临床研究进展[J].世界中西医结合杂,2009,4(2):149-152.
[4]朱萱萱,朱芳,施荣山,等.桃仁、防己提取物对大鼠血小板聚集作用的研究[J].中医药研究,2000,16(3):44-45.
[5]彭静,陈志良,梁磊,等.正交试验优选易宁更年颗粒水提工艺及药效学对比[J].中成药,2015,37(6):1350-1353.
[6]陈贝贝,仝燕,马振山,等.正交试验法优选芪术颗粒提取工艺[J].中国实验方剂学杂志,2015,21(5):46-49.
[7]周昕,谢瑞芳,顾希钧,等.多指标综合分析法优选复方自身清颗粒制备工艺[J].中成药,2015,30(10):1451-1454.
[8]王永强,陈玉威.正交试验筛选乳癖消巴布膏提取工艺的研究[J].辽宁中医杂志,2013,40(12):2547-2548.
[9]李志浩,孙新建,翟京红,等.高效液相色谱法测定复方蒲黄片中香蒲新苷与异鼠李素-3-O-新橙皮糖苷含量[J].医药导报,2012,31(4):503-505.
[10]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科学出版社,2010:261.
[11]朱建新,王宏,何薇.清热除湿颗粒提取工艺研究[J].中成药,2012,34(5):979-983.
[12]石伟,叶玉妹,唐英,等.多指标综合加权评分法考察冬柏通淋合剂提取工艺[J].辽宁中医杂志,2014,41(3):530-532.
[13]王德杭,黄有带,景运条,等.正交试验法优选金芍胶囊的水提工艺[J].中国实验方剂学杂志,2010,16(14):23-24.
[14]申洪超,陈舒茵,黎瑞汝,等.多指标综合评分法优选律复康胶囊水提工艺的研究[J].辽宁中医杂志,2010,37(9):1783-1785.
[15]周本杰,李志新,冯丽娟.正交实验优选“脂必清”的提取工艺[J].中药材,2008,31(2):300-302.
[16]吴文辉,叶美颜.多指标正交试验优选煎膏剂的水提工艺[J].中华中医药学刊,2015,33(4):948-951.