~(131)I标记苯丙氨酸方法的实验探讨
|
摘要
目的利用核素~(131)I和苯丙氨酸化学、物理的特性,寻找显像剂以及显像的载体。方法通过氯胺T的氧化反应,将~(131)I标记到苯丙氨酸上。再利用不同的扩展剂检测~(131)I-苯丙氨酸标记率。结果通过反复实验,以10%二氯甲烷+丙酮为扩展剂,~(131)I-苯丙氨酸标记率为95%以上。结论通过10%二氯甲烷+丙酮为扩展剂得到理想实验结果。
Objective To find imaging agent by using radioactive iodine characteristics of chemistry, physics, and in the human body physiological phenomenon. Methods By chloramine T oxidation reaction, to the l-phenylalanine ~(131)I marked out. Using different extension agent detection ~(131)I-l-phenylalanine marking rate. Results Our repeated experiments showed that, with 10% methylene chloride + acetone as the extension agent, ~(131)I-l-phenylalanine markup rate above 95%. Conclusion By 10% methylene chloride +acetone as the extension agent can get the ideal imaging agent.
Objective To find imaging agent by using radioactive iodine characteristics of chemistry, physics, and in the human body physiological phenomenon. Methods By chloramine T oxidation reaction, to the l-phenylalanine ~(131)I marked out. Using different extension agent detection ~(131)I-l-phenylalanine marking rate. Results Our repeated experiments showed that, with 10% methylene chloride + acetone as the extension agent, ~(131)I-l-phenylalanine markup rate above 95%. Conclusion By 10% methylene chloride +acetone as the extension agent can get the ideal imaging agent.
引文
[1]Urdancta AL,Yu JB,Wilson LD.population based cancer registryanalysis of primary tracheal carcinoma[J].Am J Clin oncol,2011,34(1):32-37.
[2]Costelloe CM,CHuang HH,Madewell JE,et al.FDG PE/CT of primary bane tumors[J].AM J Roentgenol,2014,202(6):w521-w531.
[3]Jemal A,Bray F,centen MM,et al.Global cancer statistics[J].CA Cancer Jclin,2011,61(2):69-90
[4]Sun L,Zhao L,LUO Z,et al.18F-FDG PET/CT for malignant small intestinal neoplasms[J].nUCL SCI Tech,2010,21(2):94-98.
[5]陈传新,石怡珍,杨仪,等.抗ProGRP(31-98)单克隆抗体D-D3的131I标记及期体内生物学发布研究[J].重庆医学,2011,40(8):752-754
[6]翟士军,邱玉华,万卫星,等.单克隆抗体4E5的131I标记剂131I-4E5对B细胞淋巴瘤细胞的杀伤效应[J].中华核医学与分子影像杂志2012,32(3):218-221.
[2]Costelloe CM,CHuang HH,Madewell JE,et al.FDG PE/CT of primary bane tumors[J].AM J Roentgenol,2014,202(6):w521-w531.
[3]Jemal A,Bray F,centen MM,et al.Global cancer statistics[J].CA Cancer Jclin,2011,61(2):69-90
[4]Sun L,Zhao L,LUO Z,et al.18F-FDG PET/CT for malignant small intestinal neoplasms[J].nUCL SCI Tech,2010,21(2):94-98.
[5]陈传新,石怡珍,杨仪,等.抗ProGRP(31-98)单克隆抗体D-D3的131I标记及期体内生物学发布研究[J].重庆医学,2011,40(8):752-754
[6]翟士军,邱玉华,万卫星,等.单克隆抗体4E5的131I标记剂131I-4E5对B细胞淋巴瘤细胞的杀伤效应[J].中华核医学与分子影像杂志2012,32(3):218-221.