益气生骨颗粒促进大鼠骨折愈合的实验研究
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摘要
目的:观察益气生骨颗粒对骨折愈合的促进作用。方法:选用32只3月龄大鼠,全部手术制作左侧桡骨中段1mm骨缺损的骨折模型,随机分为用药组和模型组,每组16只。造模次日,用药组予益气生骨颗粒,12 g/(kg·d),分2次灌胃;模型组予白开水,2 mL/次,2次/d。于造模后第7、14、21、28天分批处死动物,取左侧(造模侧)骨痂先拍X线观察,然后再作组织学观察;右侧(健侧)尺骨行生物力学性能检验。结果:骨痂X线观察显示用药组在14、21 d骨痂量多、密度高且缺损基本填满,而模型组多个样本缺损尚未填满、骨痂量少、密度浅淡。组织形态学观察显示造模后7 d时,用药组与模型组均无血肿存在,成纤维细胞量很少;用药组骨痂在14、21、28 d均比模型组更成熟,表现为骨小梁排列更加规则。在21、28 d,骨性组织面积高于模型组(P<0.05)。生物力学测试,健侧尺骨的载荷-位移曲线在第2周低于模型组,而在第1、3、4周两组比较,差异无统计学意义(P>0.05)。结论:益气生骨颗粒能够促进骨折愈合,表现为促进骨痂的生长,提高骨痂的钙化质量。
Objective: To observe the effect of Reinforcing Qi and Producing Bone Granules(益气生骨颗粒)on fracture healing. Methods: The thirty-two SD rats aged three months were randomized into two groups of 16 each: the model group and the therapy group. All of them were performed into left-radial 1 mm bone coloboma model on operation. The next day of operation, they were fed orally with boiled water(2 mL every time, twice a day) and "Reinforcing Qi and Producing Bone" Granules 12 g/(kg·d), twice a day. On the 7 th, 14 th, 21 st, 28 thday after operation, the animals were scarified, left radius were used to measure X-ray films and examined histomorphological of bone callus, right ulna were used to measure the biomechanics. Results: Bone callus macroscopic X-rays reveal that compared with the model group, on the 14 th, 21 stday after operation the therapy group have more of bone callus, higher density and its deficits are almost fulfilled. Tissue-morphism reveals that on the 7 thday after operation, the model group and the therapy group both have no hematoma, and have small number of fibroblasts. And bone callus of the therapy group are of ripenss much more, the array of bone trabecula are more regular, and the area of osteoid tissue in therapy group is bigger on 21 st, 28 thday(P<0.05). The biomechanics reveal that in the therapy group, the curve of download-displacement of right ulna is lower on 14 th day than that of the model group(P<0.05). Two groups have no notable distinction with each other on the 7 th,14 th, 21 st, 28 thday(P>0.05). Conclusions: "Reinforcing Qi and Producing Bone" Granules could surely promote fracture healing, and it can promote the growth of bone callus and improve the quality of calcification of bone callus.
Objective: To observe the effect of Reinforcing Qi and Producing Bone Granules(益气生骨颗粒)on fracture healing. Methods: The thirty-two SD rats aged three months were randomized into two groups of 16 each: the model group and the therapy group. All of them were performed into left-radial 1 mm bone coloboma model on operation. The next day of operation, they were fed orally with boiled water(2 mL every time, twice a day) and "Reinforcing Qi and Producing Bone" Granules 12 g/(kg·d), twice a day. On the 7 th, 14 th, 21 st, 28 thday after operation, the animals were scarified, left radius were used to measure X-ray films and examined histomorphological of bone callus, right ulna were used to measure the biomechanics. Results: Bone callus macroscopic X-rays reveal that compared with the model group, on the 14 th, 21 stday after operation the therapy group have more of bone callus, higher density and its deficits are almost fulfilled. Tissue-morphism reveals that on the 7 thday after operation, the model group and the therapy group both have no hematoma, and have small number of fibroblasts. And bone callus of the therapy group are of ripenss much more, the array of bone trabecula are more regular, and the area of osteoid tissue in therapy group is bigger on 21 st, 28 thday(P<0.05). The biomechanics reveal that in the therapy group, the curve of download-displacement of right ulna is lower on 14 th day than that of the model group(P<0.05). Two groups have no notable distinction with each other on the 7 th,14 th, 21 st, 28 thday(P>0.05). Conclusions: "Reinforcing Qi and Producing Bone" Granules could surely promote fracture healing, and it can promote the growth of bone callus and improve the quality of calcification of bone callus.
引文
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[15]王运龙,赵医琳.川芎嗪针骶管滴注治疗及预防腰椎间盘突出粘连68例疗效观察[J].中医药导报,2010,16(10):54.
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[17]赵连根.桃仁注射液对家兔缺血皮肤切口愈合的影响[J].中国中西医结合外科杂志,1996,2(2):112-115.
[18]罗纳德·W·杜德克.组织学[M].2版.瓦龙美,主译.北京:中信出版社,2003:12,31-37.
[2]吴华,陈继革,陈超.接骨七厘片治疗骨折的实验研究[J].华中科技大学学报(医学版),2003,32(1):92-94,104-122.
[3]张大方.药理与中药药理实验(供中医药类专业用)[M].上海:上海科学技术出版社,2002:8-9.
[4]马克昌,谢文,谢艳,等.益气活血冲剂对创伤骨折致肾脏损伤的保护作用[J].中国中医骨伤科杂志,2001,9(5):18-21.
[5]柴本甫,过邦辅.理气药物对骨折愈合的影响的初步研究[J].中华外科杂志,1962,10(5):299-304.
[6]符诗聪,杜宁,史炜镔,等.丹参有效部位(丹参-9403)对骨折愈合影响的生物力学实验研究[J].中国中西医结合杂志,1999,19(2):43-44.
[7]魏民.病理学[M].上海:上海科学技术出版社,1995:22.
[8]郭维淮.平乐正骨[M].北京:中国中医药出版社,1995:17-18.
[9]周重建,王绪辉,朱显华,等.益气化瘀剂在骨折愈合中的生化和病理观察[J].中国中医骨伤科杂志,1989,5(3):7-10.
[10]胡美珠,张菊英,刘季兰,等.丹参注射液对家兔桡骨骨折以后肢体血容量的影响[J].中华外科杂志,1979,17(1):33-36.
[11]马克昌,朱太永,刘鲜茹,等.益气化瘀汤对实验性骨折的影响[J].中医正骨,1995,7(1):3-6,48.
[12]方文贤,宋崇顺,周立效.医用中药药理学[M].北京:人民卫生出版社,1998:140-928.
[13]谢进,姜升平,张旭桥,等.当归补血膏治疗骨科术后血虚发热的临床观察[J].中医药导报,2013,19(11):44-46.
[14]李伦明,孙宗全.川芎嗪对器官微循环的影响[J].中国微循环杂志,1999,9(2):41-43.
[15]王运龙,赵医琳.川芎嗪针骶管滴注治疗及预防腰椎间盘突出粘连68例疗效观察[J].中医药导报,2010,16(10):54.
[16]李红红,周晓,杨荣源.川芎嗪对急性胰腺炎患者血小板活化因子的影响[J].湖南中医药导报,2002,8(11):656-657.
[17]赵连根.桃仁注射液对家兔缺血皮肤切口愈合的影响[J].中国中西医结合外科杂志,1996,2(2):112-115.
[18]罗纳德·W·杜德克.组织学[M].2版.瓦龙美,主译.北京:中信出版社,2003:12,31-37.