甘蔗与斑茅BC_1分子鉴定、抗黑穗病和花叶病初步评价
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摘要
为了解甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)杂交后代作为抗病亲本的利用价值,通过特异引物PCR鉴定出78份甘蔗与斑茅杂交BC_1真实杂种;通过人工接种花叶病毒和黑穗病菌,初步评价了甘蔗和斑茅杂交BC_1的抗病表现。结果表明,甘蔗和斑茅杂交BC_1的抗花叶病具有普遍性,而黑穗病抗性则出现分离。初步筛选出BC_1无性系YCE01-48、YCE01-71、YCE01-105、YCE01-125、YCE02-184和YCE01-118可同时抗花叶病和黑穗病,有望成为甘蔗杂交利用的高抗病源亲本。
In order to understand the utilization value of hybrids between Saccharum and Erianthus arundinaceus as resistant parents, the resistance to smut and SCMV of some progenies BC_1 from Saccharum×E. arundinaceus were evaluated by artificial inoculation of pathogens. The results showed that most of BC_1 had the resistance to SCMV, and the resistance to smut was segregative among progenies. The BC_1 clones YCE01-48, YCE01-71, YCE01-105, YCE01-125, YCE02-184 and YCE01-118 showed co-resistance to smut and SCMV. So, they could be used as parents with high disease resistance for sugarcane breeding.
In order to understand the utilization value of hybrids between Saccharum and Erianthus arundinaceus as resistant parents, the resistance to smut and SCMV of some progenies BC_1 from Saccharum×E. arundinaceus were evaluated by artificial inoculation of pathogens. The results showed that most of BC_1 had the resistance to SCMV, and the resistance to smut was segregative among progenies. The BC_1 clones YCE01-48, YCE01-71, YCE01-105, YCE01-125, YCE02-184 and YCE01-118 showed co-resistance to smut and SCMV. So, they could be used as parents with high disease resistance for sugarcane breeding.
引文
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