采精频率对德州驴精液品质的影响
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摘要
为探究采精频率对成年种公驴精液品质的影响,对东阿国家黑毛驴繁育中心34头种公驴的采精状况及生产数据进行跟踪与统计分析,并将数据分为4组,A组(每3天采精1次)、B组(每4天采精1次)、C组(每5天采精1次)和D组(每10天采精1次)。结果表明:D组的总精液量显著低于其他各组(P<0.05),C组的总精液量显著低于A组(P<0.05)和B组(P<0.05);C组精液的精子密度与D组间无显著性差异(P>0.05),但显著高于A组(P<0.05)和B组(P<0.05);D组精液的原精活力和冻后活力均显著低于其他各组(P<0.05),但A、B、C三组间差异并不显著(P>0.05);D组精子的复苏率显著低于其他各组(P<0.05),B组与C组间差异不显著(P>0.05),但均显著低于A组(P<0.05);采精频率与冻后活力、复苏率之间均呈显著负相关(P<0.05)。结论:种公驴采精频率为每3天采精1次时,可以获得稳定的精液品质。
To investigate the effect of semen collection frequency on the semen quality of donkey,the production data were analyzed to four frequencies of semen collection either once every 3 d(group A),4 d(group B),5 d(group C)and 10 d(group D). The results indicated that the semen volume in group D was significantly lower than those in other groups(P<0.05). The volume in group C was significantly higher than that in group D,but lower than those in group A and group B(P<0.05). The spermconcentrations in group C and group D had no significant difference(P >0.05),but significantly higher than those in group A and group B(P<0.05). The fresh and frozen-thawed vitality of the sperm had no significant difference in group A,group B and group C(P >0.05),but was significantly higher than that in group D(P<0.05). The frozen-thaw success rate reached maximum in group A,which was significantly higher than those in other semen collection frequencies(P<0.05). There were no significantly difference between group B and group C(P >0.05). The semen collection frequency had significantly negative correlation with the frozen-thawed vitality and recovery rate of the sperm(P<0.05). According to these studies,the semen collection frequency had a definite effect on semen quality,the best frequency of semen collection was once per 3 days so as to ensure the semen quality.
To investigate the effect of semen collection frequency on the semen quality of donkey,the production data were analyzed to four frequencies of semen collection either once every 3 d(group A),4 d(group B),5 d(group C)and 10 d(group D). The results indicated that the semen volume in group D was significantly lower than those in other groups(P<0.05). The volume in group C was significantly higher than that in group D,but lower than those in group A and group B(P<0.05). The spermconcentrations in group C and group D had no significant difference(P >0.05),but significantly higher than those in group A and group B(P<0.05). The fresh and frozen-thawed vitality of the sperm had no significant difference in group A,group B and group C(P >0.05),but was significantly higher than that in group D(P<0.05). The frozen-thaw success rate reached maximum in group A,which was significantly higher than those in other semen collection frequencies(P<0.05). There were no significantly difference between group B and group C(P >0.05). The semen collection frequency had significantly negative correlation with the frozen-thawed vitality and recovery rate of the sperm(P<0.05). According to these studies,the semen collection frequency had a definite effect on semen quality,the best frequency of semen collection was once per 3 days so as to ensure the semen quality.
引文
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[16] Mosenene T M B. Characterization and cryopreservation of semen of four South African chicken breeds[D]. University of the Free State,2009.
[17] Du Plessis S S,Mc Allister D A,Luu A,et al. Effects of H2O2exposure on human sperm motility parameters,reactive oxygen species levels and nitric oxide levels[J]. Andrologia,2010,42(3):206-210.
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[19] Baumber J,Ball B A,Gravance C G,et al. The effect of reactive oxygen species on equine sperm motility,viability,acrosomal integrity,mitochondrial membrane potential,and membrane lipid peroxidation[J]. Journal of Andrology,2000,21(6):895-902.
[2]孙玉江,徐纪尊,蒋涛,等.中国驴种遗传资源保护利用研究[J].中国草食动物,2006,26(6):32-34.
[3]张淑珍,孙爱林,李守富,等.我国肉驴产业现状与展望[J].贵州畜牧兽医,2016,40(4):37-38.
[4]曾维斌,王艳萍.马、驴精液保存研究进展[J].中国草食动物,2009,29(4):59-62.
[5] Demyda-Peyrás S,Bottrel M,Acha D,et al. Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa[J].Animal Reproduction Science,2018,193:201-208.
[6]张瑞涛.德州驴冷冻精液及其主要影响因素研究[D].济南:山东师范大学,2014.
[7]张瑞涛,李敏,尹桂军,等.应用卵黄液4℃保存驴精液效果及受孕率研究[J].黑龙江畜牧兽医,2016(5下):78-79.
[8] Miclea V,Gyorgy I,Miclea I,et al. Influence of collection frequency on the quality of ejaculates from Large White and Pietrain boars[J].Bulletin UASVM Animal Science and Biotechnologies,2016,73:2.
[9] Pruneda A,Pinart E,Briz M D,et al. Effects of a high semen-collection frequency on the quality of sperm from ejaculates and from six epididymal regions in boars[J]. Theriogenology,2005,63(8):2219-2232.
[10]张瑞涛,张燕,张帅,等.德州驴体尺,性行为与精液品质的相关性研究[J].家畜生态学报,2016,37(6):14-18.
[11] Kaya A,Aksoy M,Tekeli T. Influence of ejaculation frequency on sperm characteristics,ionic composition and enzymatic activity of seminal plasma in rams[J]. Small Ruminant Research,2002,44(2):153-158.
[12] De Jonge C,La Fromboise M,Bosmans E,et al. Influence of the abstinence period on human sperm quality[J]. Fertility and Sterility,2004,82(1):57-65.
[13] Noirault J,Brillard J P. Effects of frequency of semen collection on quantitative and qualitative characteristics of semen in turkey breeder males[J]. Poultry Science,1999,78(7):1034-1039.
[14] Nwachukwu E N,Ibe S N,Amadi C U. Effect of genotype and frequency of semen collection on semen characteristics of local chicken cocks[J]. Journal of Animal and Veterinary Advances,2006,5(7):562-565.
[15] Savic'R,Petrovic'M. Variability in ejaculation rate and libido of boars during reproductive exploitation[J]. South African Journal of Animal Science,2015,45(4):355-361.
[16] Mosenene T M B. Characterization and cryopreservation of semen of four South African chicken breeds[D]. University of the Free State,2009.
[17] Du Plessis S S,Mc Allister D A,Luu A,et al. Effects of H2O2exposure on human sperm motility parameters,reactive oxygen species levels and nitric oxide levels[J]. Andrologia,2010,42(3):206-210.
[18] Savic'R,Petrovic'M,Radojkovic'D,et al. Effect of photoperiod and frequency of ejaculation on sperm traits of boars[C]//Proceedings of the International Syiposium on Animal Science.2016:122-127.
[19] Baumber J,Ball B A,Gravance C G,et al. The effect of reactive oxygen species on equine sperm motility,viability,acrosomal integrity,mitochondrial membrane potential,and membrane lipid peroxidation[J]. Journal of Andrology,2000,21(6):895-902.