不同光质对苦荞芽生长及品质的影响
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摘要
为探讨不同光质对苦荞芽生长及品质的影响,明确最适宜的光照条件,选用西荞I号做为试验材料,采用红光(RED)、蓝光(BLUE)、LED、1.5RED/FAR、0.5RED/FAR 5种光质,研究了不同光照条件下苦荞发芽过程中芽生长、黄酮含量及抗氧化活性的变化.结果表明:光照处理(BLUE、0.5RED/FAR)显著影响苦荞芽的生长,芽的鲜重、茎长和茎粗较对照处理(黑暗)分别降低了2.8%～41.3%、7.3%～50.2%、2.0%～25.0%;与对照相比,在第10d,1.5RED/FAR处理提高了苦荞芽对DPPH和ABTS自由基的清除率,分别提高了0.6%和3.9%;光照处理(1.5RED/FAR和0.5RED/FAR)显著增加了苦荞芽的总黄酮和芦丁的含量,与对照相比总黄酮含量分别提高了1.8%和22.8%,芦丁含量分别提高了2.9%、23.9%;光照处理(BLUE、1.5RED/FAR、LED)显著增加了苦荞芽的槲皮素含量,分别提高了52.3%、50.8%、55.3%.
Xiqiao No.1 is selected as the test material.These five kinds of light(RED light,BLUE light,LED light,1.5 RED/FAR and 0.5 RED/FAR)are used to study the changes of bud growth,flavonoids content and antioxidant activity of Buckwheat during germination under different light conditions.The results show that light treatment(BLUE,0.5 RED/FAR)significantly affects the Buckwheat bud growth,shoot fresh weight,stem length and stem diameter.Compared with those from the control(DARK)group,all Buckwheat bud growth,shoot fresh weight,stem length and stem diameter are reduced by 2.8% ～41.3%,7.3% ～50.2%,2.0% ～25.0%,respectively.Compared with the control group,the 1.5 RED/FAR treatment enhances the scavenging rate of DPPH and ABTS radicals by Buckwheat seedlings in the tenth day by 0.6% and 3.9%.Light treatment(1.5 RED/FAR and 0.5 RED/FAR)significantly increases the content of Buckwheat flavonoids and rutin.Compared with the control group,the total flavonoids content increases by 1.8% and 22.8% and the content of rutin increases by 2.9% and 23.9%.Light treatment(BLUE,1.5 RED/FAR,LED)significantly increases the content of quercetin in Buckwheat buds by 52.3%,50.8% and 55.3%.
Xiqiao No.1 is selected as the test material.These five kinds of light(RED light,BLUE light,LED light,1.5 RED/FAR and 0.5 RED/FAR)are used to study the changes of bud growth,flavonoids content and antioxidant activity of Buckwheat during germination under different light conditions.The results show that light treatment(BLUE,0.5 RED/FAR)significantly affects the Buckwheat bud growth,shoot fresh weight,stem length and stem diameter.Compared with those from the control(DARK)group,all Buckwheat bud growth,shoot fresh weight,stem length and stem diameter are reduced by 2.8% ～41.3%,7.3% ～50.2%,2.0% ～25.0%,respectively.Compared with the control group,the 1.5 RED/FAR treatment enhances the scavenging rate of DPPH and ABTS radicals by Buckwheat seedlings in the tenth day by 0.6% and 3.9%.Light treatment(1.5 RED/FAR and 0.5 RED/FAR)significantly increases the content of Buckwheat flavonoids and rutin.Compared with the control group,the total flavonoids content increases by 1.8% and 22.8% and the content of rutin increases by 2.9% and 23.9%.Light treatment(BLUE,1.5 RED/FAR,LED)significantly increases the content of quercetin in Buckwheat buds by 52.3%,50.8% and 55.3%.
引文
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[17]崔琳琳,周一鸣,季红斌,等.不同光质对苦荞萌发过程中的黄酮类化合物及其抗氧化活性的影响[J].食品工业科技,2016,37(6):104-108.
[2]阎红.荞麦的应用研究及展望[J].食品工业科技,2011,32(1):363-365.
[3]侯雪梅,袁仲.荞麦的营养保健功能与开发利用[J].农产品加工(学刊),2014,13(1):73-75.
[4]朱云辉,郭元新.我国苦荞资源的开发利用研究进展[J].食品工业科技,2014,36(24):360-365.
[5]吉牛拉惹.不同光照强度对苦荞麦主要生物学性状的影响[J].安徽农业科学,2008,36(16):6638-6639,6641.
[6]胡阳,江莎,李洁,等.光强和光质对植物生长发育的影响[J].内蒙古农业大学学报(自然科学版),2009,30(4):296-301.
[7]许大全,高伟,阮军.光质对植物生长发育的影响[J].植物生理学报,2015,51(8):1217-1234.
[8]Hua J S,Dai H Y.Effects of light intensity on growth,development and formation on fagopyrum cymosum[J].Agr Sci Technol,2014,15(3):363-366.
[9]陈志,孙庆丽,汪一婷,等.不同光质对油菜幼苗生长的影响[J].农业工程,2013,3(6):143-149.
[10]Khatami F,Ghanati F.Effects of UV irradiation on cell viability,anthocyanin,and flavonoid contents of callus-cultured Malva neglecta cell[C]//2011 International Conference on Life Science and Technology.Singapore:IACSIT Press,2011.
[11]Zhana L J,Hub J Q,Panga L Y,et al.Light exposure reduced browning enzyme activity and accumulated total phenols in cauliflower heads during cool storage[J].Posth Biol Technol,2014,88:17-20.
[12]郭银生,谷艾素,崔瑾.光质对水稻幼苗生长及生理特性的影响[J].应用生态学报,2011,22(6):1485-1492.
[13]王静波,赵江林,彭镰心,等.苦荞芽中黄酮类化合物含量及其抗氧化性的研究[J].现代食品科技,2013,29(5):965-968.
[14]郭徽,宾婕,刘洁,等.苦荞中总黄酮的含量测定[J].云南中医中药杂志,2011,32(1):57-58.
[15]邹亮,王战国,胡慧玲,等.苦荞提取物中芦丁和槲皮素的含量测定[J].中国实验方剂学杂志,2010,16(17):60-62.
[16]李韶山,潘瑞炽.植物的蓝光效应[J].植物生理学通讯,1993,29(4):248-252.
[17]崔琳琳,周一鸣,季红斌,等.不同光质对苦荞萌发过程中的黄酮类化合物及其抗氧化活性的影响[J].食品工业科技,2016,37(6):104-108.