冠菌素诱导冬小麦幼苗抗旱性的浓度效应
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摘要
为检验冠菌素(COR)喷施诱导植物抗旱性的效果,选用两个冬小麦品种济麦22和良星99为材料,分析了不同浓度COR下冬小麦水培幼苗抗旱性的变化。结果表明,在20%PEG-6000的模拟干旱条件下,喷施低浓度(≤1μmol.L-1)的COR增加了小麦幼苗的株高、根长和干物重,提高了叶片叶绿素含量和根系活力,增加了叶片相对含水量、超氧化物歧化酶活性及游离脯氨酸、可溶性糖、可溶性蛋白等渗透调节物质含量;而喷施高浓度(≥10μmol.L-1)的COR溶液则抑制了幼苗生长和生理代谢活性。说明喷施适宜浓度的COR(1～10μmol.L-1)对冬小麦抗旱性有一定的诱导作用。
Coronatine(COR) is one kind of phytotoxin,secreted by Pseudomonas syringae and is similar to the structure of JA,and could induce the plant resistance with the low concentration.The inductive effects on drought resistance of spraying different COR concentrations was studied using two winter wheat cultivars Jimai 22 and Liangxi 99 as experimental materials in a hydroponic experiment.The results shows that,in the condition of 20% PEG-6000 simulated water stress,spraying lower concentration of COR solution(≤1 μmol·L-1) could increase the plant height,root length and dry matter weight of the wheat seedlings,increased the leaf chlorophyll content and root activity,improved the relative water content and the contents of osmosis regulators such as proline,soluble sugar and protein,and increased the SOD activity and decrease MDA content.On the contrary,spraying higher COR solution(≥10 μmol·L-1)would restrain the seedling growth and activities of physiological metabolism.So in conclusion,spraying COR at suitable concentration could increase the drought resistance of wheat.
Coronatine(COR) is one kind of phytotoxin,secreted by Pseudomonas syringae and is similar to the structure of JA,and could induce the plant resistance with the low concentration.The inductive effects on drought resistance of spraying different COR concentrations was studied using two winter wheat cultivars Jimai 22 and Liangxi 99 as experimental materials in a hydroponic experiment.The results shows that,in the condition of 20% PEG-6000 simulated water stress,spraying lower concentration of COR solution(≤1 μmol·L-1) could increase the plant height,root length and dry matter weight of the wheat seedlings,increased the leaf chlorophyll content and root activity,improved the relative water content and the contents of osmosis regulators such as proline,soluble sugar and protein,and increased the SOD activity and decrease MDA content.On the contrary,spraying higher COR solution(≥10 μmol·L-1)would restrain the seedling growth and activities of physiological metabolism.So in conclusion,spraying COR at suitable concentration could increase the drought resistance of wheat.
引文
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[22]Li X W,Shen X F.Coronatine alleviates water deficiencystress on winter wheat seedlings[J].Journal of IntegrativePlant Biology,2010,52(7):616-625.
[2]Neumann D,Nieden U Z,Lichtenberger O,et al.How doesarmeria maritima tolerate high heavy-metal concentrations[J].Journal of Plant Physiology,1995,146(5-6):704-717.
[3]Smirnoff N.Plant resistance to environmental stress[J].Cur-rent Opinion in Biotechnology,1998,9(2):214-219.
[4]Ruan L,Chen L J,Chen Y H,et al.Expression of Arabidopsishome domain glabrous11enhances tolerance to drought stressin transgenic sweet potato plants[J].Journal of Plant Biolo-gy,2012,55(2):151-158.
[5]Pinheiro C,Antonio C,Ortuno M F,et al.Initial water deficiteffects on lupinus albus photosynthetic performance,carbonmetabolism,and hormonal balance:metabolic reorganizationprior to early stress responses[J].Journal of ExperimentalBotany,2011,62(14):4965-4974.
[6]Zhao M R,Han Y Y,Feng Y N,et al.Expansins are involvedin cell growth mediated by abscisic acid and indole-3-aceticacid under drought stress in wheat[J].Plant Cell Reports,2012,31(4):671-685.
[7]齐付国,李建民,段留生,等.冠菌素对小麦几个抗寒生理指标的影响[J].麦类作物学报,2006,26(6):149-153.
[8]艾林,李召虎,李建民,等.冠菌素对旱稻和水稻种子萌发的生理效应[J].华北农学报,2008,23(3):77-80.
[9]秦欣,孙世玲,王铭伦,等.干旱胁迫下冠菌素对花生幼苗叶片渗透调节物质及膜脂过氧化的影响[J].花生学报,2009,38(1):18-21.
[10]周于毅,沈雪峰,张明才,等.冠菌素诱导小麦幼苗抗高温胁迫的生理机制[J].麦类作物学报,2011,31(1):139-142.
[11]卫晓轶,张明才,李召虎,等.冠菌素对玉米节间伸长和产量性状的影响[J].科技导报,2011,29(20):63-67.
[12]王蕾,王倩,李召虎,等.植物生长物质冠菌素提高黄瓜幼苗耐冷性的效应[J].中国农业大学学报,2006,11(6):45-48.
[13]张志勇,田晓莉,李建民,等.冠菌素在不同供钾水平下诱导棉花侧根发生的效应及机理[C]//中国棉花学会2006年年会暨第七次代表大会论文汇编.
[14]Cerrudo I,Keller Mercedes M M,Cargnel M D,et al.LowRed:Far-red ratios reduce arabidopsis resistance to botrytiscinerea and Jasmonate responses via a COI1-JAZ1O-depend-ent,salicylic acid-independent mechanism[J].Plant Physiol-ogy,2012,158(4):2042-2052.
[15]Wu H L,Wu X L,Li Z H,et al.Physiological evaluation ofdrought stress tolerance and recovery in cauliflower(Brassi-ca oleracea L.)seedlings treated with Methyl jasmonate andcoronatine[J].Journal of Plant Growth Regulation,2012,31(1):113-123.
[16]张志良,翟伟菁,植物生理学实验指导[M].北京:高等教育出版社,2003:40-41.
[17]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[18]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999:303.
[19]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999:95-96.
[20]Zeng W,He S Y.A prominent role of the flagellin receptorFLAGELLIN-SENSING2in mediating stomatal response topseudomonas syringae pv tomato DC3000in Arabidopsis[J].Plant Physiology,2010,153(3):1188-1198.
[21]Fernández Aguirre C,Daga Ruiz D,Velasco Garrido J L,etal.Cor pulmonale-PHT secondary to crest syndrome treat-ment with slow continuous ultrafiltration[J].Medicina In-tensiva,2011,34(9):629-631.
[22]Li X W,Shen X F.Coronatine alleviates water deficiencystress on winter wheat seedlings[J].Journal of IntegrativePlant Biology,2010,52(7):616-625.