HPLC-ELSD测定银杏叶刺梨胶囊中银杏萜类内酯的含量
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摘要
建立高效液相色谱-蒸发光散射检测器法(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)同时测定保健食品银杏叶刺梨胶囊中银杏萜类内酯含量的方法。色谱柱为Waters Symmetry C18色谱柱(250 mm×4.6 mm,5μm),柱温为35℃;流动相为甲醇-水=30∶70(体积比),流速1.0 mL/min;ELSD检测器雾化温度100℃,雾化气为空气,气流速度2.5 L/min。白果内酯、银杏内酯A、银杏内酯B、银杏内酯C分别在4.376μg～3.128μg,4.176μg～12.528μg,4.320μg～12.960μg,4.368μg～13.104μg之间线性关系良好,平均加样回收率分别为100.86%、101.52%、99.60%、100.69%。该方法可准确测定银杏叶刺梨胶囊中银杏萜类内酯的含量,可用于该产品的质量分析。
A HPLC-ELSD method was developed to determine the content of Ginkgo Terpene Lactones in health food ginkgo leaf and Rosa roxburghii capsule. The HPLC was performed on Waters Symmetry C18(250 mm × 4.6 mm,5 μm)column,mobile phase consisted methanol-H2 O(30 ∶ 70,volume ratio),column temperature was set at 35 ℃,and flow rate was 1.0 mL/min. The temperature of drift tube was 100 ℃ and the flow rate of gas was 2.5 L/min. A good linearity was obtained in the range of 4.376 μg-13.128 μg of bilobalide,4.176 μg-12.528 μg of ginkgolide A,4.320 μg-12.960 μg of ginkgolide B,4.368 μg-13.104 μg of ginkgolide C. The average recovery rates of bilobalide,ginkgolide A,ginkgolide B,and ginkgolide C were 100.86 %,101.52 %,99.60 %,and 100.69 %,respectively. The established method was suitable for quality control of ginkgo leaf and Rosa roxburghii capsule.
A HPLC-ELSD method was developed to determine the content of Ginkgo Terpene Lactones in health food ginkgo leaf and Rosa roxburghii capsule. The HPLC was performed on Waters Symmetry C18(250 mm × 4.6 mm,5 μm)column,mobile phase consisted methanol-H2 O(30 ∶ 70,volume ratio),column temperature was set at 35 ℃,and flow rate was 1.0 mL/min. The temperature of drift tube was 100 ℃ and the flow rate of gas was 2.5 L/min. A good linearity was obtained in the range of 4.376 μg-13.128 μg of bilobalide,4.176 μg-12.528 μg of ginkgolide A,4.320 μg-12.960 μg of ginkgolide B,4.368 μg-13.104 μg of ginkgolide C. The average recovery rates of bilobalide,ginkgolide A,ginkgolide B,and ginkgolide C were 100.86 %,101.52 %,99.60 %,and 100.69 %,respectively. The established method was suitable for quality control of ginkgo leaf and Rosa roxburghii capsule.
引文
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[2]潘苏华,牛文明,吴丽,等.银杏叶提取物与刺梨配伍抗实验性肝损伤作用[J].药学实践杂志, 2009, 27(6):437-438
[3]徐艳芬,张丽娟,宋新波.银杏叶提取物的研究进展[J].药物评价研究, 2010, 33(6):452-456
[4]张鹏飞,廖丽君,邓祯,等.银杏叶提取物的药理作用及其临床应用研究进展[J].辽宁中医杂志, 2017, 44(2):426-429
[5]耿婷,申文雯,王佳佳,等.银杏叶中内酯类成分的研究进展[J].中国中药杂志,2018,43(7):1384-1391
[6]王旋,张慧灵,顾振纶,等.银杏内酯药理作用的研究进展[J].中草药,2005,36(11):1741-1744
[7]袁传勋,潘见,胡学桥,等.HPLC-RI法测定银杏叶提取物中4种内酯的含量[J].药物分析杂志,2008,28(5):739-741
[8]刘红梅,周庆霞,杨文玲.毛细管气相色谱法测定银杏叶中萜内酯含量[J].河北科技大学学报,2006,27(3):209-213
[9]吴憩,姚鑫.UPLC-MS-MS测定不同厂家银杏叶提取物中4种萜内酯的含量[J].中国实验方剂学杂志,2016,22(15):60-63
[10]冯海容.基于一测多评法的银杏叶分散片的溶出度研究[J].中国药师,2017,20(3):551-556
[11]孙婷.HPLC法测定银刺胶囊中总黄酮的含量[J].食品工业科技,2005,26(4):169-17
[12]盛志恒,李旭红.HPLC-ELSD法测定银杏叶提取物中四种萜类内酯的含量[J].浙江中医学院学报,2004,28(4):82-83
[13]朱雪妍,罗轶,谷立勍.HPLC-ELSD法测定银杏洋参颗粒中萜类内酯的含量[J].广西中医药大学学报,2014,17(1):83-85
[14]国家药典委员会.中华人民共和国药典一部[S].北京:中国医药科技出版社,2015:416-417