猫豆脂溶性成分及抗氧化活性研究
|
摘要
目的:探讨猫豆中脂溶性成分以及其抗氧化活性能力。方法:依次经过95%乙醇回流提取、石油醚萃取、萃取浓缩物甲酯化处理,然后利用GC-MS联用技术进行分析和鉴定,并通过标准质谱谱库定性,最后采用清除DPPH和ABTS+自由基能力的实验检验其抗氧化活性能力。结果:共检出19个成分并全部鉴定,对DPPH和ABTS+自由基的半数清除率浓度IC50值分别为46.62 mg·mL-1、41.51 mg·mL-1。结论:猫豆脂溶性成分以脂肪酸为主,并具有一定的抗氧化活性能力,且呈现良好的线性关系。
Objective:To investigate the liposoluble constituents and their antioxidant activities in of Maodou(velvet bean).Methods:After 95% ethanol reflux extraction,petroleum ether extraction and extraction of methyl esterification,GC-MS was used to analyze and identify the liposoluble constituents,and qualitative analysis was carried out by standard mass spectral library.Finally,DPPH and ABTS+free radicals was used to test its antioxidant activity.Results:A total of 19 constituents were detected and identified.The IC50 values of DPPH and ABTS+free radical capacity were 46.62 mg·mL-1 and 41.51 mg·mL-1.Conclusion:The liposoluble constituents of Maodou(velvet bean) are mainly fatty acid identified with an antioxidant capacity,and it has a good linear relationship.
Objective:To investigate the liposoluble constituents and their antioxidant activities in of Maodou(velvet bean).Methods:After 95% ethanol reflux extraction,petroleum ether extraction and extraction of methyl esterification,GC-MS was used to analyze and identify the liposoluble constituents,and qualitative analysis was carried out by standard mass spectral library.Finally,DPPH and ABTS+free radicals was used to test its antioxidant activity.Results:A total of 19 constituents were detected and identified.The IC50 values of DPPH and ABTS+free radical capacity were 46.62 mg·mL-1 and 41.51 mg·mL-1.Conclusion:The liposoluble constituents of Maodou(velvet bean) are mainly fatty acid identified with an antioxidant capacity,and it has a good linear relationship.
引文
[1]蒋伟哲,周燕文,吴闯,等.不同产地猫豆中左旋多巴的含量比较[J].中草药,2000,31(11):860-862.
[2]张凤银,陈禅友.藜豆的研究进展[J].江汉大学学报,2012,40(6):87-89.
[3]巫世红,蒋伟哲,吕力,等.猫豆药材的质量标准研究[J].中药材,2009,32(3):333-336.
[4]巫世,陈勇,蒋伟哲,等.猫豆的鉴别研究[J].中药材,2009,32(5):693-396.
[5]邱卓,蒋伟哲,巫玲玲,等.猫豆营养成分的初步研究[J].广西中医学院学报,2011,14(4):48-51.
[6]巫玲玲.猫豆化学成分研究[D].南宁:广西医科大学,2011:1-64.
[7]张赟赟,杨海船,李嘉,等.瑶药马蹄蕨中脂溶性成分的GC-MS分析[J].中国药房,2015,26(18):2544-2546.
[8]郭雪峰,岳永德,汤锋,等.用清除有机自由基DPPH法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(7):1578-1582.
[9]勾明玥,刘梁,张春枝.采用DPPH法测定26种植物的抗氧化活性[J].食品与发酵工业,2010,36(3):148-150.
[10]胡耀辉,于晓明,王玉华,等.不同豆浆制备工艺活性成分与ABTS自由基清除能力比较研究[J].粮油加工(电子版),2015(4):47-51.
[11]吴春岚,潘卫东,刘云,等.猫豆的现代研究进展[J].上海中医药大学学报,2011,25(5):104-107.
[12]张春娥,张惠,刘楚恰,等.亚油酸的研究进展[J].技术·油脂工程,2010(5):18-21.
[2]张凤银,陈禅友.藜豆的研究进展[J].江汉大学学报,2012,40(6):87-89.
[3]巫世红,蒋伟哲,吕力,等.猫豆药材的质量标准研究[J].中药材,2009,32(3):333-336.
[4]巫世,陈勇,蒋伟哲,等.猫豆的鉴别研究[J].中药材,2009,32(5):693-396.
[5]邱卓,蒋伟哲,巫玲玲,等.猫豆营养成分的初步研究[J].广西中医学院学报,2011,14(4):48-51.
[6]巫玲玲.猫豆化学成分研究[D].南宁:广西医科大学,2011:1-64.
[7]张赟赟,杨海船,李嘉,等.瑶药马蹄蕨中脂溶性成分的GC-MS分析[J].中国药房,2015,26(18):2544-2546.
[8]郭雪峰,岳永德,汤锋,等.用清除有机自由基DPPH法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(7):1578-1582.
[9]勾明玥,刘梁,张春枝.采用DPPH法测定26种植物的抗氧化活性[J].食品与发酵工业,2010,36(3):148-150.
[10]胡耀辉,于晓明,王玉华,等.不同豆浆制备工艺活性成分与ABTS自由基清除能力比较研究[J].粮油加工(电子版),2015(4):47-51.
[11]吴春岚,潘卫东,刘云,等.猫豆的现代研究进展[J].上海中医药大学学报,2011,25(5):104-107.
[12]张春娥,张惠,刘楚恰,等.亚油酸的研究进展[J].技术·油脂工程,2010(5):18-21.