热应激状态下不同品种肉牛免疫功能和抗氧化功能的比较研究
|
摘要
为探究热应激对内蒙古草原不同品种放牧肉牛免疫功能和抗氧化功能的影响,利用酶联免疫法对处于热应激状态下的不同品种放牧肉牛进行了血清免疫指标和抗氧化指标的测定。结果表明:在热应激状态,蒙古牛的白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、超氧化物歧化酶(SOD)及总抗氧化能力(T-AOC)含量显著高于西门塔尔牛和安格斯牛(P<0.05),而西门塔尔牛和安格斯牛之间的差异不显著(P>0.05);蒙古牛的丙二醛(MDA)含量显著低于西门塔尔牛和安格斯牛(P<0.05);安格斯牛和西门塔尔牛的免疫球蛋白A(IgA)和免疫球蛋白M(IgM)含量有高于蒙古牛的趋势,但差异不显著(P>0.05),以上试验牛之间免疫球蛋白G(IgG)含量无显著差异(P>0.05)。综上,在热应激状态下,蒙古牛的免疫功能和抗氧化功能较强,耐热应激能力强于西门塔尔牛和安格斯牛。
The aim of this study was to explore the effects of heat stress on the immune and antioxidant functions of different breeds of grazing beef cattle in Inner Mongolia grassland.Serum immune and antioxidant indexes of different breeds of grazing beef cattle under heat stress were determined by enzyme-linked immunosorbent assay(ELISA).The results showed that the contents of interleukin-2(IL-2),interleukin-4(IL-4),superoxide dismutase(SOD) and total antioxidant capacity(T-AOC) in Mongolian cattle were significantly higher than those in Simmental cattle and Angus cattle(P <0.05),but there was no significant difference between Simmental cattle and Angus cattle(P>0.05).The malondialdehyde(MDA) content of Mongolian cattle was significantly lower than that of Simmental cattle and Angus cattle(P<0.05),while the immunoglobulin A(IgA) and IgM(IgM) contents of Angus cattle and Simmental cattle were higher than those of Mongolian cattle,but the difference was not significant(P>0.05).And the IgG content had no significant difference(P>0.05).In conclusion,under heat stress,the Mongolian cattle had stronger immune function and antioxidant function,and its heat stress resistance was stronger than Simmental and Angus cattle.
The aim of this study was to explore the effects of heat stress on the immune and antioxidant functions of different breeds of grazing beef cattle in Inner Mongolia grassland.Serum immune and antioxidant indexes of different breeds of grazing beef cattle under heat stress were determined by enzyme-linked immunosorbent assay(ELISA).The results showed that the contents of interleukin-2(IL-2),interleukin-4(IL-4),superoxide dismutase(SOD) and total antioxidant capacity(T-AOC) in Mongolian cattle were significantly higher than those in Simmental cattle and Angus cattle(P <0.05),but there was no significant difference between Simmental cattle and Angus cattle(P>0.05).The malondialdehyde(MDA) content of Mongolian cattle was significantly lower than that of Simmental cattle and Angus cattle(P<0.05),while the immunoglobulin A(IgA) and IgM(IgM) contents of Angus cattle and Simmental cattle were higher than those of Mongolian cattle,but the difference was not significant(P>0.05).And the IgG content had no significant difference(P>0.05).In conclusion,under heat stress,the Mongolian cattle had stronger immune function and antioxidant function,and its heat stress resistance was stronger than Simmental and Angus cattle.
引文
[1] 宋小珍,付戴波,瞿明仁,杨食堂,刘道杨,徐振松.热应激对肉牛血清内分泌激素含量、抗氧化酶活性及生理生化指标的影响[J].动物营养学报,2012,24(12):2485-2490Song X Z,Fu D B,Qu M R,Yang S T,Liu D Y,Xu Z S.Effects of heat stress on endocrine hormone content,antioxidant enzyme activity and physiological and serum biochemical indices of beef cattle[J].Animal Nutrition,2012,24(12):2485-2490 (in Chinese)
[2] 蒲启建,王之盛,彭全辉,张灿,景小平,胡瑞,邹华围.热应激对不同品种(系)青年肉牛生产性能、营养物质表观消化率及血液生化指标的影响[J].动物营养学报,2017,29(9):3120-3131Pu Q J,Wang Z S,Peng Q H,Zhang C,Jing X P,Hu R,Zou H W.Effects of heat stress on performance,nutrient apparent digestibility and blood biochemical indices of different breeds of young beef cattle[J].Animal Nutrition,2017,29(9):3120-3131 (in Chinese)
[3] 白丹丹.不同季节对三河牛血液生化指标及冷热应激相关基因表达的影响[D].呼和浩特:内蒙古农业大学,2017Bai D D.Effects of different seasons on blood biochemical indexes and related genes expression of cold and heat stress in Sanhe cattle[D].Hohhot:Inner Mongolia Agricultural University,2010 (in Chinese)
[4] 兰玉坤.内蒙古地区近50年气候变化特征研究[D].北京:中国农业科学院,2007Lan Y K.Characteristics of climate change in Inner Mongolia in the past 50 years[D].Beijing:Chinese Academy of Agricultural Sciences,2007 (in Chinese)
[5] 钟光,邵丹,胡艳,施寿荣,宋志刚,童海兵.持续热应激对黄羽肉鸡生长性能、器官指数、血清生化指标和抗氧化功能的影响[J].动物营养学报,2018,30(11):4425-4432Zhong G,Shao D,Hu Y,Shi S R,Song Z G,Tong H B,Effects of persistent heat stress on grow performance,organ indices,serum biochemical indices and antioxidant function of yellow-feathered broilers[J].Journal of Animal Nutrition,2018,30(11):4425-4432 (in Chinese)
[6] Gregory N G.How climatic changes could affect meat quality[J].Food Research International,2010,43(7):1866-1873
[7] 熊云霞,马现永,郑春田,田志梅,张健,陈伟,胡友军,王丽.热应激对猪肠道健康、免疫系统和肉品质的影响及作用机制[J].动物营养学报,2017,29(2):374-381Xiong Y X,Ma X Y,Zheng C T,Tian Z M,Zhang J,Chen W,Hu Y J,Wang L.Effects of heat stress on intestinal health,immune system and meat quality in pigs and its mechanisms[J].Chinese Journal of Animal Nutrition,2017,29(2):374-381 (in Chinese)
[8] 夏东,杨淑晶,刘铁铮.奶牛血液抗氧化酶活性的季节变化[J].家畜生态学报,2006,27(1):56-58Xia D,Yang S J,Liu T Z.Seasonal changes of antioxidant enzymes in dairy cows[J].Journal of Domestic Animal Ecology,2006,27(1):56-58 (in Chinese)
[9] Silanikove N.Effects of heat stress on the welfare of extensively managed domestic ruminants[J].Livestock Production Science,2000,67(1-2):1-18
[10] 畅旺东.放牧条件下蒙古牛血液中抗氧化、免疫及代谢相关指标的研究[D].呼和浩特:内蒙古农业大学,2017Chang W D.Study on antioxidation,immunity and metabolism related indicators on blood of Mongolia cattle under the grazing condition[D].Hohhot:Inner Mongolia Agricultural University,2017 (in Chinese)
[11] 高锦萍,赵青萍,缪敏.高热惊厥患儿免疫球蛋白的变化[J].苏州大学学报,2006,26(6):1089-1091Gao J P,Zhao Q P,Miu M.Changes of immunoglobulin in children with febrile convulsions[J].Journal of Soochow University,2006,26(6):1089-1091 (in Chinese)
[12] 刘庆华.娟荷杂交牛耐热性能研究[D].南京:南京农业大学,2009Liu Q H.Study on heat tolerance of Jersey×Holstein dairy cows[D].Nanjing:Nanjing Agricultural University,2009 (in Chinese)
[13] 陈杰.家畜生理学[M].第3版.北京:中国农业出版社,2008Chen J.Physiology of Domestic Animals[M].Beijing:China Agricultural Press,2003 (in Chinese)
[14] 刘维权,动物生物化学实验指导[M].第3版.北京:中国农业出版社,2008Liu W Q.Guidance for Animal Biochemistry Experiments[M].3rd ed.Beijing:China Agricultural Press,2008 (in Chinese)
[15] 蔡明成.热应激对肉牛生理生化指标及外周血microRNA表达水平的影响[D].重庆:西南大学,2014Cai M C.Effects of heat stress on physiological and biochemical indexes and expression levels of microRNA in peripheral blood[D].Chongqing:Southwest University,2014 (in Chinese)
[16] Paul A,Chrystal M P,Mojgan A,Akgul A,Douglas C P,Noriko S,Andrew K,Christian S H,Christian H,Christopher A K,Yutaka T,Nicholas P R.Interleukin-2-dependent mechanisms of tolerance and immunity in vivo[J].Journal of Immunology,2006,176(9):5255-5266
[17] Cremaschi G A,Gorelik G,Klecha A J,Lysionek A E,Genaro A M.Chronic stress influences the immune system through the thyroid axis[J].Life Science,2000,67(26):3171-3179
[18] Eck J B,Kuhn C M.Effect of ether stress on growth hormone during development in the neonatal rat[J].Neuroendocrinology,1992,56(5):605-610
[19] Johnson H D,Vanjonack W J.Effects of environmental and other stressors on blood hormone patterns in lactating animals[J].Journal of Dairy Science,1976,59(9):1603-1617
[20] Siegel H S,Latimer J W.Interaction of high temperature and Salmonella pullorum antigen concentration on serum agglutinin and corticosteroid responses in White Rock chickens[J].Poultry Science,1984,63(12):2483-2491
[21] 崔恒敏,陈怀涛.动物红细胞免疫功能的研究进展[J].中国兽医科技,2003,33(5):23-27Cui H M,Chen H T.Research progress of animal erythrocyte immune function[J].Chinese Veterinary Science and Technology,2003,33(5):23-27 (in Chinese)
[22] 陈雯雯,李津,郑威,程龙,邓慧芳,莫小贵,杨炳壮,何宝祥.高温高湿对奶水牛生理和抗氧化指标的影响[J].广西畜牧兽医,2012,28(4):226-228Chen W W,Li J,Zheng W,Cheng L,Deng H F,Mo X G,Yang B Z,He B X.Effects of high temperature and humidity on physiological and antioxidant indices of buffalo milk[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine,2012,28(4):226-228 (in Chinese)
[23] 李忠浩,孔丽娟,刘庆华,杜娟,狄和双,王根林.不同温度下荷斯坦奶牛外周血抗氧化指标的变化及其与淋巴细胞凋亡的关系[J].中国奶牛,2007,增刊:136-139Li Z H,Kong L J,Liu Q H,Du J,Di H S,Wang G L.Changes of antioxidant indices in peripheral blood of Holstein cows at different temperatures and their relationship with lymphocyte apoptosis[J].China Diary Cattle,2007(S):136-139 (in Chinese)
[2] 蒲启建,王之盛,彭全辉,张灿,景小平,胡瑞,邹华围.热应激对不同品种(系)青年肉牛生产性能、营养物质表观消化率及血液生化指标的影响[J].动物营养学报,2017,29(9):3120-3131Pu Q J,Wang Z S,Peng Q H,Zhang C,Jing X P,Hu R,Zou H W.Effects of heat stress on performance,nutrient apparent digestibility and blood biochemical indices of different breeds of young beef cattle[J].Animal Nutrition,2017,29(9):3120-3131 (in Chinese)
[3] 白丹丹.不同季节对三河牛血液生化指标及冷热应激相关基因表达的影响[D].呼和浩特:内蒙古农业大学,2017Bai D D.Effects of different seasons on blood biochemical indexes and related genes expression of cold and heat stress in Sanhe cattle[D].Hohhot:Inner Mongolia Agricultural University,2010 (in Chinese)
[4] 兰玉坤.内蒙古地区近50年气候变化特征研究[D].北京:中国农业科学院,2007Lan Y K.Characteristics of climate change in Inner Mongolia in the past 50 years[D].Beijing:Chinese Academy of Agricultural Sciences,2007 (in Chinese)
[5] 钟光,邵丹,胡艳,施寿荣,宋志刚,童海兵.持续热应激对黄羽肉鸡生长性能、器官指数、血清生化指标和抗氧化功能的影响[J].动物营养学报,2018,30(11):4425-4432Zhong G,Shao D,Hu Y,Shi S R,Song Z G,Tong H B,Effects of persistent heat stress on grow performance,organ indices,serum biochemical indices and antioxidant function of yellow-feathered broilers[J].Journal of Animal Nutrition,2018,30(11):4425-4432 (in Chinese)
[6] Gregory N G.How climatic changes could affect meat quality[J].Food Research International,2010,43(7):1866-1873
[7] 熊云霞,马现永,郑春田,田志梅,张健,陈伟,胡友军,王丽.热应激对猪肠道健康、免疫系统和肉品质的影响及作用机制[J].动物营养学报,2017,29(2):374-381Xiong Y X,Ma X Y,Zheng C T,Tian Z M,Zhang J,Chen W,Hu Y J,Wang L.Effects of heat stress on intestinal health,immune system and meat quality in pigs and its mechanisms[J].Chinese Journal of Animal Nutrition,2017,29(2):374-381 (in Chinese)
[8] 夏东,杨淑晶,刘铁铮.奶牛血液抗氧化酶活性的季节变化[J].家畜生态学报,2006,27(1):56-58Xia D,Yang S J,Liu T Z.Seasonal changes of antioxidant enzymes in dairy cows[J].Journal of Domestic Animal Ecology,2006,27(1):56-58 (in Chinese)
[9] Silanikove N.Effects of heat stress on the welfare of extensively managed domestic ruminants[J].Livestock Production Science,2000,67(1-2):1-18
[10] 畅旺东.放牧条件下蒙古牛血液中抗氧化、免疫及代谢相关指标的研究[D].呼和浩特:内蒙古农业大学,2017Chang W D.Study on antioxidation,immunity and metabolism related indicators on blood of Mongolia cattle under the grazing condition[D].Hohhot:Inner Mongolia Agricultural University,2017 (in Chinese)
[11] 高锦萍,赵青萍,缪敏.高热惊厥患儿免疫球蛋白的变化[J].苏州大学学报,2006,26(6):1089-1091Gao J P,Zhao Q P,Miu M.Changes of immunoglobulin in children with febrile convulsions[J].Journal of Soochow University,2006,26(6):1089-1091 (in Chinese)
[12] 刘庆华.娟荷杂交牛耐热性能研究[D].南京:南京农业大学,2009Liu Q H.Study on heat tolerance of Jersey×Holstein dairy cows[D].Nanjing:Nanjing Agricultural University,2009 (in Chinese)
[13] 陈杰.家畜生理学[M].第3版.北京:中国农业出版社,2008Chen J.Physiology of Domestic Animals[M].Beijing:China Agricultural Press,2003 (in Chinese)
[14] 刘维权,动物生物化学实验指导[M].第3版.北京:中国农业出版社,2008Liu W Q.Guidance for Animal Biochemistry Experiments[M].3rd ed.Beijing:China Agricultural Press,2008 (in Chinese)
[15] 蔡明成.热应激对肉牛生理生化指标及外周血microRNA表达水平的影响[D].重庆:西南大学,2014Cai M C.Effects of heat stress on physiological and biochemical indexes and expression levels of microRNA in peripheral blood[D].Chongqing:Southwest University,2014 (in Chinese)
[16] Paul A,Chrystal M P,Mojgan A,Akgul A,Douglas C P,Noriko S,Andrew K,Christian S H,Christian H,Christopher A K,Yutaka T,Nicholas P R.Interleukin-2-dependent mechanisms of tolerance and immunity in vivo[J].Journal of Immunology,2006,176(9):5255-5266
[17] Cremaschi G A,Gorelik G,Klecha A J,Lysionek A E,Genaro A M.Chronic stress influences the immune system through the thyroid axis[J].Life Science,2000,67(26):3171-3179
[18] Eck J B,Kuhn C M.Effect of ether stress on growth hormone during development in the neonatal rat[J].Neuroendocrinology,1992,56(5):605-610
[19] Johnson H D,Vanjonack W J.Effects of environmental and other stressors on blood hormone patterns in lactating animals[J].Journal of Dairy Science,1976,59(9):1603-1617
[20] Siegel H S,Latimer J W.Interaction of high temperature and Salmonella pullorum antigen concentration on serum agglutinin and corticosteroid responses in White Rock chickens[J].Poultry Science,1984,63(12):2483-2491
[21] 崔恒敏,陈怀涛.动物红细胞免疫功能的研究进展[J].中国兽医科技,2003,33(5):23-27Cui H M,Chen H T.Research progress of animal erythrocyte immune function[J].Chinese Veterinary Science and Technology,2003,33(5):23-27 (in Chinese)
[22] 陈雯雯,李津,郑威,程龙,邓慧芳,莫小贵,杨炳壮,何宝祥.高温高湿对奶水牛生理和抗氧化指标的影响[J].广西畜牧兽医,2012,28(4):226-228Chen W W,Li J,Zheng W,Cheng L,Deng H F,Mo X G,Yang B Z,He B X.Effects of high temperature and humidity on physiological and antioxidant indices of buffalo milk[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine,2012,28(4):226-228 (in Chinese)
[23] 李忠浩,孔丽娟,刘庆华,杜娟,狄和双,王根林.不同温度下荷斯坦奶牛外周血抗氧化指标的变化及其与淋巴细胞凋亡的关系[J].中国奶牛,2007,增刊:136-139Li Z H,Kong L J,Liu Q H,Du J,Di H S,Wang G L.Changes of antioxidant indices in peripheral blood of Holstein cows at different temperatures and their relationship with lymphocyte apoptosis[J].China Diary Cattle,2007(S):136-139 (in Chinese)