高效液相色谱-串联质谱检测酱油中纳他霉素
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摘要
采用高效液相色谱-串联质谱法对酱油中纳他霉素进行检测。以Kromasil 100-5C18柱3.9mm×150mm为色谱柱,以甲醇、10mmol/L乙酸铵为流动相。通过HPLC-MS/MS系统中全扫描模式对纳他霉素进行定性分析,多反应监测模式对其进行定量分析。结果表明:纳他霉素在0.025~1μg/mL内具有良好线性,检出限为5.14μg/kg,加标回收率均在79.4%~93.1%。结论:该方法操作方便、专属性强、灵敏度高、准确度高,可用于酱油中纳他霉素的检测。
Develop a detection method for natamycin in soy sauce using high performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Use Kromasil 100-5 C_(18) column 3.9 mm×150 mm as the chromatographic column,and methanol,10 mmol/L ammonium acetate as the mobile phase.Qualitative analysis of natamycin is conducted under full-scan model,and quantitative analysis is conducted in the multiple reaction monitoring(MRM)model.The results show that the calibration curve for the analyte shows good linearity in the range of 0.025~1μg/mL.The limit of detection is 5.14μg/kg,and the recoveries obtained by standard addition method are in the range of 79.4%~93.1%.Conclution:This method is simple,with strong specificity,high accuracy and high sensitivity.It can be used for detecting natamycin added in soy sauce.
Develop a detection method for natamycin in soy sauce using high performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Use Kromasil 100-5 C_(18) column 3.9 mm×150 mm as the chromatographic column,and methanol,10 mmol/L ammonium acetate as the mobile phase.Qualitative analysis of natamycin is conducted under full-scan model,and quantitative analysis is conducted in the multiple reaction monitoring(MRM)model.The results show that the calibration curve for the analyte shows good linearity in the range of 0.025~1μg/mL.The limit of detection is 5.14μg/kg,and the recoveries obtained by standard addition method are in the range of 79.4%~93.1%.Conclution:This method is simple,with strong specificity,high accuracy and high sensitivity.It can be used for detecting natamycin added in soy sauce.
引文
[1]GB 2760-2014,食品添加剂使用标准[S].
[2]李东,杜连祥,路福平,等.纳他霉素的抑菌谱及最小抑菌浓度[J].食品工业科技,2004(7):143-144.
[3]王志强,胡国媛,李志勇,等.微生物抑制法快速检测鲜奶中多种抗生素残留[J].中国食品卫生杂志,2008,20(2):139-141.
[4]Vallvey C.Rapid ultraviolet spectrophotometric and liquid chromatographic methods for the determination of natamycin in Lactoserum matrix[J].J AOAC Int,2000,83(4):802-808.
[5]王克利,张宣义,刘醇,等.HPLC法测定沙拉酱和干酪中纳他霉素含量方法的研究[J].中国卫生检验杂志,1998(6):380-381.
[6]骆健美,金志华,岑沛霖.RP-HPLC法测定发酵液中纳他霉素含量[J].中国抗生素杂志,2004,29(11):653-656.
[7]陈永艳,李彩瑞,杨秀敏,等.高效液相色谱测定果酱中的纳他霉素[J].中国食品学报,2008,8(1):115-118.
[8]周玉虎,赵慧芬.导数光谱法测定酸奶中的纳他霉素[J].中国乳品工业,2008,36(8):54-55.
[9]申国华,董培智,裴社强,等.HPLC-MS-MS法测定降糖类中成药中非法添加物格列波脲[J].中成药,2014,36(6):1213-1217.
[10]林芳,李涛,耿庆光,等.超高效液相色谱-二级管阵列检测器法快速测定保健食品中违法添加的14种性功能药物[J].食品科学,2014,35(4):163-169.
[11]朱峰,阮丽萍,马永健,等.超高效液相色谱-串联质谱联用法同时检测降糖类和减肥类保健品中20种非法添加的化学降糖药物[J].色谱,2014,32(1):13-20.
[12]沈国芳,朱建,裘一静.UPLC-MS-MS检测减肥类保健食品中的西布曲明及其甲基衍生物[J].中国药学杂志,2011,46(20):1609-1611.
[13]吴杨,张圆,闵春艳,等.辅助降血糖类保健食品非法添加化学物质检测方法研究[J].中国执业药师,2014,11(10):11-14.
[2]李东,杜连祥,路福平,等.纳他霉素的抑菌谱及最小抑菌浓度[J].食品工业科技,2004(7):143-144.
[3]王志强,胡国媛,李志勇,等.微生物抑制法快速检测鲜奶中多种抗生素残留[J].中国食品卫生杂志,2008,20(2):139-141.
[4]Vallvey C.Rapid ultraviolet spectrophotometric and liquid chromatographic methods for the determination of natamycin in Lactoserum matrix[J].J AOAC Int,2000,83(4):802-808.
[5]王克利,张宣义,刘醇,等.HPLC法测定沙拉酱和干酪中纳他霉素含量方法的研究[J].中国卫生检验杂志,1998(6):380-381.
[6]骆健美,金志华,岑沛霖.RP-HPLC法测定发酵液中纳他霉素含量[J].中国抗生素杂志,2004,29(11):653-656.
[7]陈永艳,李彩瑞,杨秀敏,等.高效液相色谱测定果酱中的纳他霉素[J].中国食品学报,2008,8(1):115-118.
[8]周玉虎,赵慧芬.导数光谱法测定酸奶中的纳他霉素[J].中国乳品工业,2008,36(8):54-55.
[9]申国华,董培智,裴社强,等.HPLC-MS-MS法测定降糖类中成药中非法添加物格列波脲[J].中成药,2014,36(6):1213-1217.
[10]林芳,李涛,耿庆光,等.超高效液相色谱-二级管阵列检测器法快速测定保健食品中违法添加的14种性功能药物[J].食品科学,2014,35(4):163-169.
[11]朱峰,阮丽萍,马永健,等.超高效液相色谱-串联质谱联用法同时检测降糖类和减肥类保健品中20种非法添加的化学降糖药物[J].色谱,2014,32(1):13-20.
[12]沈国芳,朱建,裘一静.UPLC-MS-MS检测减肥类保健食品中的西布曲明及其甲基衍生物[J].中国药学杂志,2011,46(20):1609-1611.
[13]吴杨,张圆,闵春艳,等.辅助降血糖类保健食品非法添加化学物质检测方法研究[J].中国执业药师,2014,11(10):11-14.