去细胞组织水凝胶化的研究进展
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摘要
通过去细胞组织可制备细胞外基质Extracellular matrix,ECM生物支架,在临床应用中大量应用于构建结构化和功能化的组织模型,ECM材料易溶解,可与水凝胶材料很好的融合,不论是体外作为培养支架材料,还是体内作为注射填充材料,都避免了形状无规则的缺点。然而ECM水凝胶促进细胞分化、增殖的机制尚未完全研究清楚,但是与天然材料保留了许多结构和生物学信号有很大关系。该文献初步阐述了ECM水凝胶的制备、部分物理、化学和生物学特性等研究进展,以及在心、脑两种器官的体内临床应用,初步讨论了ECM水凝胶的某些研究和应用前景。
Extracellular matrix bioscaffolds prepared from decellularized tissues have been used in the constructive and functional tissue remodeling in a variety of clinical applications. The ECM materials are easily soluble and subsequently combine well with hydrogels and can be used as bioscaffolds for tissue culture in vitro, or as filling materials for injection in vivo because they can overcome the defects of irregular shape during the manipulations. The mechanisms by which ECM hydrogels facilitate cell differentiation and proliferation are only partially understood, but likely relate to the structural substances from the natural tissues and biological signal. This review elaborates the progresses on preparation, some physical and biological characteristics of ECM hydrogels, their clinical application in vivo, in heart and brain, and preliminarily discusses some research directions and clinical application of ECM hydrogels.
Extracellular matrix bioscaffolds prepared from decellularized tissues have been used in the constructive and functional tissue remodeling in a variety of clinical applications. The ECM materials are easily soluble and subsequently combine well with hydrogels and can be used as bioscaffolds for tissue culture in vitro, or as filling materials for injection in vivo because they can overcome the defects of irregular shape during the manipulations. The mechanisms by which ECM hydrogels facilitate cell differentiation and proliferation are only partially understood, but likely relate to the structural substances from the natural tissues and biological signal. This review elaborates the progresses on preparation, some physical and biological characteristics of ECM hydrogels, their clinical application in vivo, in heart and brain, and preliminarily discusses some research directions and clinical application of ECM hydrogels.
引文
[1] Deng XQ,Chao NN,Ding W,Qin TW,Wang W,Zhang Y,Luo JC.Production and Characterization of Composite Chitosan Hydrogel Containing Extracellular Matrix Particles for Tissue Engineering Applications[J].J Biomed Nanotechnol,2019,15(4):756-768.
[2] Choi JS,Kim BS,Kim JD,Choi YC,Lee HY,Cho YW.In vitro cartilage tissue engineering using adipose-derived extracellular matrix scaffolds seeded with adipose-derived stem cells[J].Tissue Engineering Part A,2012,(18):80-92.
[3] Mercuri JJ,Gill SS,Simionescu DT.Novel tissue-derived biomimetic scaffold for regenerating the human nucleus pulposus[J].J Biomed Mater Res A,2011,(96):422-435.
[4] Mercuri JJ,Patnaik S,Dion G,Gill SS,Liao J,Simionescu DT.Regenerative potential of decellularized porcine nucleus pulposus hydrogel scaffolds:stem cell differentiation,matrix remodeling,and biocompatibility studies[J].Tissue Eng Part A,2013,(19):952-966.
[5] Wassenaar JW,Braden RL,Osborn KG,Christman KL.Modulating in vivo degradation rate of injectable extracellular matrix hydrogels[J].J Mater Chem B,2016,(4):2794-2802.
[6] Hof KS,Bastings MMC.Programmable Control in Extracellular Matrix- mimicking Polymer Hydrogels[J].Chimia (Aarau),2017,71(6):342-348.
[7] Saheli M,Sepantafar M,Pournasr B,Farzaneh Z,Vosough M,Piryaei A,Baharvand H.Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function[J].J Cell Biochem,2018,119(6):4320-4333.
[8] Saldin LT,Cramer MC,Velankar SS,White LJ,Badylak SF.Extracellular matrix hydrogels from decellularized tissues:Structure and function[J].Acta Biomater,2017,(49):1-15.
[9] Badylak SF,Freytes DO,Gilbert TW.Extracellular matrix as a biological scaffold material:Structure and function[J].Acta Biomater,2009,(5):1-13.
[10] Ghuman H,Massensini AR,Donnelly J,Kim SM,Medberry CJ,Badylak SF,Modo M.ECM hydrogel for the treatment of stroke:Characterization of the host cell infiltrate[J].Biomaterials,2016,(91):166-181.
[11] Massensini AR,Ghuman H,Saldin LT,Medberry CJ,Keane TJ,Nicholls FJ,Velankar SS,Badylak SF,Modo M.Concentration-dependent rheological properties of ECM hydrogel for intracerebral delivery to a stroke cavity[J].Acta Biomater,2015,(27):116-130.
[12] Wolf MT,Daly KA,Brennan-Pierce EP,Johnson SA,Carruthers CA,D'Amore A,Nagarkar SP,Velankar SS,Badylak SF.A hydrogel derived from decellularized dermal extracellular matrix[J].Biomaterials,2012,(33):7028-7038.
[13] Zhang L,Zhang F,Weng Z,Brown BN,Yan H,Ma XM,Vosler PS,Badylak SF,Dixon CE,Cui XT,Chen J.Effect of an inductive hydrogel composed of urinary bladder matrix upon functional recovery following traumatic brain injury[J].Tissue Eng Part A,2013,(19):1909-1918.
[14] Bible E,Dell'Acqua F,Solanky B,Balducci A,Crapo PM,Badylak SF,Ahrens ET,Modo M.Noninvasive imaging of transplanted human neural stem cells and ECM scaffold remodeling in the stroke-damaged rat brain by (19)F- and diffusion-MRI[J].Biomaterials,2012,(33):2858-2871.
[15] Fisher MB,Liang R,Jung HJ,Kim KE,Zamarra G,Almarza AJ,McMahon PJ,Woo SL.Potential of healing a transected anterior cruciate ligament with genetically modified extracellular matrix bioscaffolds in a goat model[J].Knee Surg Sports Traumatol Arthrosc,2012,(20):1357-1365.
[2] Choi JS,Kim BS,Kim JD,Choi YC,Lee HY,Cho YW.In vitro cartilage tissue engineering using adipose-derived extracellular matrix scaffolds seeded with adipose-derived stem cells[J].Tissue Engineering Part A,2012,(18):80-92.
[3] Mercuri JJ,Gill SS,Simionescu DT.Novel tissue-derived biomimetic scaffold for regenerating the human nucleus pulposus[J].J Biomed Mater Res A,2011,(96):422-435.
[4] Mercuri JJ,Patnaik S,Dion G,Gill SS,Liao J,Simionescu DT.Regenerative potential of decellularized porcine nucleus pulposus hydrogel scaffolds:stem cell differentiation,matrix remodeling,and biocompatibility studies[J].Tissue Eng Part A,2013,(19):952-966.
[5] Wassenaar JW,Braden RL,Osborn KG,Christman KL.Modulating in vivo degradation rate of injectable extracellular matrix hydrogels[J].J Mater Chem B,2016,(4):2794-2802.
[6] Hof KS,Bastings MMC.Programmable Control in Extracellular Matrix- mimicking Polymer Hydrogels[J].Chimia (Aarau),2017,71(6):342-348.
[7] Saheli M,Sepantafar M,Pournasr B,Farzaneh Z,Vosough M,Piryaei A,Baharvand H.Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function[J].J Cell Biochem,2018,119(6):4320-4333.
[8] Saldin LT,Cramer MC,Velankar SS,White LJ,Badylak SF.Extracellular matrix hydrogels from decellularized tissues:Structure and function[J].Acta Biomater,2017,(49):1-15.
[9] Badylak SF,Freytes DO,Gilbert TW.Extracellular matrix as a biological scaffold material:Structure and function[J].Acta Biomater,2009,(5):1-13.
[10] Ghuman H,Massensini AR,Donnelly J,Kim SM,Medberry CJ,Badylak SF,Modo M.ECM hydrogel for the treatment of stroke:Characterization of the host cell infiltrate[J].Biomaterials,2016,(91):166-181.
[11] Massensini AR,Ghuman H,Saldin LT,Medberry CJ,Keane TJ,Nicholls FJ,Velankar SS,Badylak SF,Modo M.Concentration-dependent rheological properties of ECM hydrogel for intracerebral delivery to a stroke cavity[J].Acta Biomater,2015,(27):116-130.
[12] Wolf MT,Daly KA,Brennan-Pierce EP,Johnson SA,Carruthers CA,D'Amore A,Nagarkar SP,Velankar SS,Badylak SF.A hydrogel derived from decellularized dermal extracellular matrix[J].Biomaterials,2012,(33):7028-7038.
[13] Zhang L,Zhang F,Weng Z,Brown BN,Yan H,Ma XM,Vosler PS,Badylak SF,Dixon CE,Cui XT,Chen J.Effect of an inductive hydrogel composed of urinary bladder matrix upon functional recovery following traumatic brain injury[J].Tissue Eng Part A,2013,(19):1909-1918.
[14] Bible E,Dell'Acqua F,Solanky B,Balducci A,Crapo PM,Badylak SF,Ahrens ET,Modo M.Noninvasive imaging of transplanted human neural stem cells and ECM scaffold remodeling in the stroke-damaged rat brain by (19)F- and diffusion-MRI[J].Biomaterials,2012,(33):2858-2871.
[15] Fisher MB,Liang R,Jung HJ,Kim KE,Zamarra G,Almarza AJ,McMahon PJ,Woo SL.Potential of healing a transected anterior cruciate ligament with genetically modified extracellular matrix bioscaffolds in a goat model[J].Knee Surg Sports Traumatol Arthrosc,2012,(20):1357-1365.