青春期氰戊菊酯暴露对雄性大鼠睾丸组织氧化应激的影响
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摘要
目的·观察青春期氰戊菊酯暴露对雄性大鼠睾丸组织氧化应激的影响。方法·将50只Sprague-Dawley(SD)雄性大鼠随机分为对照(玉米油)组、低剂量(0.02 mg/kg氰戊菊酯)组、中剂量(1 mg/kg氰戊菊酯)组、高剂量(50 mg/kg氰戊菊酯)组及干预(50 mg/kg氰戊菊酯+100 mg/kg N-乙酰基-L-半胱氨酸)组,每组10只,于4周龄开始连续染毒2个月。测定睾丸组织中丙二醛(malondialdehyde,MDA)含量、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxide dismutase,SOD)活力,并观察大鼠睾丸组织形态结构。结果·与对照组相比,高剂量组大鼠睾丸组织中MDA含量升高,体质量、GSH-Px和SOD活力均下降(均P<0.05);而干预组与高剂量组相比,MDA含量降低,GSH-Px活力升高(均P<0.05)。睾丸组织形态学观察发现,随着染毒剂量的增加,睾丸生精细胞排列疏松,细胞层数减少,曲细精管管腔内径增大。结论·青春期氰戊菊酯暴露可能导致雄性大鼠睾丸组织氧化损伤。
Objective·To study the effects of fenvalerate exposure during puberty on oxidative stress in rat testis. Methods·Fifty male Sprague-Dawley(SD) rats were randomly divided into the control group(corn oil), low dose group(0.02 mg/kg fenvalerate), moderate dose group(1 mg/kg fenvalerate), high dose group(50 mg/kg fenvalerate) and intervention group(50 mg/kg fenvalerate+100 mg/kg N-acetyl-L-cysteine), ten rats for each group, for two months by gavage at four weeks of age. Malondialdehyde(MDA) content, activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in testis and testicular tissue morphology were detected. Results·Compared with the control group, the rat body weight and activities of GSH-Px and SOD in testis were significantly decreased in high dose group while MDA content was increased(all P<0.05). Compared with the high dose group, MDA content was decreased and GSH-Px activity was increased in the intervention group(both P<0.05). The results of testicular histology showed that with the increasing exposure dose, the spermatogenic cells were arranged loosely, the number of layers was decreased and the inner diameter of seminiferous tubules was increased. Conclusion·Exposure to fenvalerate during puberty may induce oxidative damage in testis tissue of male rats.
Objective·To study the effects of fenvalerate exposure during puberty on oxidative stress in rat testis. Methods·Fifty male Sprague-Dawley(SD) rats were randomly divided into the control group(corn oil), low dose group(0.02 mg/kg fenvalerate), moderate dose group(1 mg/kg fenvalerate), high dose group(50 mg/kg fenvalerate) and intervention group(50 mg/kg fenvalerate+100 mg/kg N-acetyl-L-cysteine), ten rats for each group, for two months by gavage at four weeks of age. Malondialdehyde(MDA) content, activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in testis and testicular tissue morphology were detected. Results·Compared with the control group, the rat body weight and activities of GSH-Px and SOD in testis were significantly decreased in high dose group while MDA content was increased(all P<0.05). Compared with the high dose group, MDA content was decreased and GSH-Px activity was increased in the intervention group(both P<0.05). The results of testicular histology showed that with the increasing exposure dose, the spermatogenic cells were arranged loosely, the number of layers was decreased and the inner diameter of seminiferous tubules was increased. Conclusion·Exposure to fenvalerate during puberty may induce oxidative damage in testis tissue of male rats.
引文
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[19]Wang XZ,Liu SS,Sun Y,et al.β-cypermethrin impairs reproductive function in male mice by inducing oxidative stress[J].Theriogenology,2009,72(5):599-611.
[2]姚克文.拟除虫菊酯农药的男(雄)性生殖毒性研究进展[J].中华男科学杂志,2008,14(3):268-271.
[3]周礼华,刘春芳,陈冲,等.氯氰菊酯对雄性小鼠小脑组织氧化应激的影响[J].包头医学院学报,2014,30(4):12-15.
[4]Casida JE,Quistad GB.Golden age of insecticide research:past,present,or future?[J].Annu Rev Entomol,1998,43:1-16.
[5]Saillenfait AM,Ndiaye D,SabatéJP.Pyrethroids:exposure and health effectsan update[J].Int J Hyg Environ Health,2015,218(3):281-292.
[6]Meeker JD,Barr DB,Hauser R.Pyrethroid insecticide metabolites are associated with serum hormone levels in adult men[J].Reprod Toxicol,2009,27(2):155-160.
[7]黄海凤,周炳,赵美蓉,等.拟除虫菊酯类农药对哺乳动物神经毒理的研究进展[J].农药学学报,2007,9(3):209-214.
[8]Yuan C,Wang C,Gao SQ,et al.Effects of permethrin,cypermethrin and3-phenoxybenzoic acid on rat sperm motility in vitro evaluated with computerassisted sperm analysis[J].Toxicol In Vitro,2010,24(2):382-386.
[9]Xia Y,Han Y,Wu B,et al.The relation between urinary metabolite of pyrethroid insecticides and semen quality in humans[J].Fertil Steril,2008,89(6):1743-1750.
[10]刘冰,马栋,毛鹏飞.氧化应激损伤在男性不育症中的影响[J].中华男科学杂志,2014,20(10):927-931.
[11]樊华,李文.雄性生殖氧化应激损伤的研究进展[J].中国临床医学,2016,23(2):242-246.
[12]陈庆,赵宗霞,赵金燕,等.百草枯对雄性大鼠精液质量及睾丸组织脂质过氧化的影响[J].环境与职业医学,2017,34(4):337-340.
[13]Zhang H,Wang H,Wang Q,et al.Pubertal and early adult exposure to fenvalerate disrupts steroidogenesis and spermatogenesis in mice at adulthood[J].J Appl Toxicol,2010,30(4):369-377.
[14]Zhao XF,Wang Q,Ji YL,et al.Fenvalerate induces germ cell apoptosis in mouse testes through the Fas/Fas L signaling pathway[J].Arch Toxicol,2011,85(9):1101-1108.
[15]李堂林,翁治委,周少虎.生殖系统氧化应激损伤的动物实验方法研究进展[J].医学综述,2015,21(6):991-994.
[16]毛毳,张丹,王成芳.氧化应激性损伤对不育男性精子功能的影响[J].四川大学学报,2010,41(4):723-724.
[17]武阳,常青,杨旭.不同浓度甲醛致大鼠肝细胞DNA氧化损伤作用[J].环境科学学报,2009,9(11):2415-2419.
[18]赵乾龙.丙烯腈诱导的氧化应激对雄性大鼠睾丸细胞NF-κB信号通路影响的研究[D].兰州:兰州大学,2016.
[19]Wang XZ,Liu SS,Sun Y,et al.β-cypermethrin impairs reproductive function in male mice by inducing oxidative stress[J].Theriogenology,2009,72(5):599-611.