摘要
以4种辣椒为材料,研究不同Na Cl胁迫浓度(0, 10 mg/L, 5 mg/L, 100 mg/L和150 mg/L)和胁迫时间(3 d, 6 d, 9 d, 12 d和15 d)下POD同工酶谱带和活性的动态变化。不同辣椒材料适应胁迫的过程及所耐受的NaCl浓度有显著差异,胁迫的前3 d内各品种POD同工酶对不同的NaCl浓度响应变化较剧烈,此后经过约10 d反复调节逐渐适应了胁迫。随着Na Cl胁迫浓度的增加及胁迫时间的延长,电泳图上半区主要发生某些酶带新增与消失,下半区发生酶活强度增强与减弱的变化。至胁迫第15天,材料1004-5和1129-2g-2在各胁迫浓度下的酶活性均高于对照,其中1004-5在10 mg/L、50 mg/L处理下增高更显著,分别为对照的1.51和1.49倍;1129-2g-2在50 mg/L达到最高,是对照的1.27倍;XJ968在10~100 mg/L浓度下酶活性高于对照,其中10 mg/L处理酶活性最高,为对照的1.47倍;1128-2p-2只在50 mg/L和100 mg/L浓度下酶活性略有增强,是对照的1.02和1.07倍。综合分析与动态比较表明在设计浓度范围内,1004-5和1129-2g-2耐受性最强,XJ968次之,1128-2p-2最弱。
Dynamic changes of POD isoenzyme patterns activities in four cultivars of pepper were studied under different concentration of Na Cl stress(0, 10 mg/L, 50 mg/L, 100 mg/L, and 150 mg/L) and stress times(3 d, 6 d, 9 d,12 d, and 15 d). The process of different pepper materials to stress and the tolerant range to concentration of NaCl were significantly different. The response of POD isoenzyme to different NaCl concentration in the first 3 days of stress was more intense, and after 10 days' adjustment, it gradually adapted to the coercion. With the increase of NaCl stress concentration and the prolongation of stress time, some bands were newly added or disappeared in the upper half of the electrophoretic pattern, and the changes of enzyme activity increased or decreased in the lower half. When stressed to 15 th day, the activity of the materials 1004-5 and 1129-2 g-2 at each stress concentration was higher than that of the control, and 1004-5 increased significantly at 10 mg/L, 50 mg/L, and each to 1.51 and1.49 times of the control; 1129-2 g-2 reached the highest at 50 mg/L, which was 1.27 times of the control. The activity of XJ968 was higher than that of the control at 10~100 mg/L, the activity of 10 mg/L was the highest,1.47 times of the control; 1128-2 p-2 only at 50 mg/L and 100 mg/L concentration of enzyme activity increased slightly, 1.02 and 1.07 times of the control. The comprehensive analysis and dynamic comparison showed that1004-5 and 1129-2 g-2 might be the most tolerant in the design concentration range, followed by XJ968, and the weakest was 1128-2 p-2.
引文
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