NaCl胁迫下4种辣椒POD同工酶谱及活性分析
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摘要
以4种辣椒为材料,研究不同Na Cl胁迫浓度(0, 10 mg/L, 5 mg/L, 100 mg/L和150 mg/L)和胁迫时间(3 d, 6 d, 9 d, 12 d和15 d)下POD同工酶谱带和活性的动态变化。不同辣椒材料适应胁迫的过程及所耐受的NaCl浓度有显著差异,胁迫的前3 d内各品种POD同工酶对不同的NaCl浓度响应变化较剧烈,此后经过约10 d反复调节逐渐适应了胁迫。随着Na Cl胁迫浓度的增加及胁迫时间的延长,电泳图上半区主要发生某些酶带新增与消失,下半区发生酶活强度增强与减弱的变化。至胁迫第15天,材料1004-5和1129-2g-2在各胁迫浓度下的酶活性均高于对照,其中1004-5在10 mg/L、50 mg/L处理下增高更显著,分别为对照的1.51和1.49倍;1129-2g-2在50 mg/L达到最高,是对照的1.27倍;XJ968在10～100 mg/L浓度下酶活性高于对照,其中10 mg/L处理酶活性最高,为对照的1.47倍;1128-2p-2只在50 mg/L和100 mg/L浓度下酶活性略有增强,是对照的1.02和1.07倍。综合分析与动态比较表明在设计浓度范围内,1004-5和1129-2g-2耐受性最强,XJ968次之,1128-2p-2最弱。
Dynamic changes of POD isoenzyme patterns activities in four cultivars of pepper were studied under different concentration of Na Cl stress(0, 10 mg/L, 50 mg/L, 100 mg/L, and 150 mg/L) and stress times(3 d, 6 d, 9 d,12 d, and 15 d). The process of different pepper materials to stress and the tolerant range to concentration of NaCl were significantly different. The response of POD isoenzyme to different NaCl concentration in the first 3 days of stress was more intense, and after 10 days' adjustment, it gradually adapted to the coercion. With the increase of NaCl stress concentration and the prolongation of stress time, some bands were newly added or disappeared in the upper half of the electrophoretic pattern, and the changes of enzyme activity increased or decreased in the lower half. When stressed to 15 th day, the activity of the materials 1004-5 and 1129-2 g-2 at each stress concentration was higher than that of the control, and 1004-5 increased significantly at 10 mg/L, 50 mg/L, and each to 1.51 and1.49 times of the control; 1129-2 g-2 reached the highest at 50 mg/L, which was 1.27 times of the control. The activity of XJ968 was higher than that of the control at 10～100 mg/L, the activity of 10 mg/L was the highest,1.47 times of the control; 1128-2 p-2 only at 50 mg/L and 100 mg/L concentration of enzyme activity increased slightly, 1.02 and 1.07 times of the control. The comprehensive analysis and dynamic comparison showed that1004-5 and 1129-2 g-2 might be the most tolerant in the design concentration range, followed by XJ968, and the weakest was 1128-2 p-2.
Dynamic changes of POD isoenzyme patterns activities in four cultivars of pepper were studied under different concentration of Na Cl stress(0, 10 mg/L, 50 mg/L, 100 mg/L, and 150 mg/L) and stress times(3 d, 6 d, 9 d,12 d, and 15 d). The process of different pepper materials to stress and the tolerant range to concentration of NaCl were significantly different. The response of POD isoenzyme to different NaCl concentration in the first 3 days of stress was more intense, and after 10 days' adjustment, it gradually adapted to the coercion. With the increase of NaCl stress concentration and the prolongation of stress time, some bands were newly added or disappeared in the upper half of the electrophoretic pattern, and the changes of enzyme activity increased or decreased in the lower half. When stressed to 15 th day, the activity of the materials 1004-5 and 1129-2 g-2 at each stress concentration was higher than that of the control, and 1004-5 increased significantly at 10 mg/L, 50 mg/L, and each to 1.51 and1.49 times of the control; 1129-2 g-2 reached the highest at 50 mg/L, which was 1.27 times of the control. The activity of XJ968 was higher than that of the control at 10～100 mg/L, the activity of 10 mg/L was the highest,1.47 times of the control; 1128-2 p-2 only at 50 mg/L and 100 mg/L concentration of enzyme activity increased slightly, 1.02 and 1.07 times of the control. The comprehensive analysis and dynamic comparison showed that1004-5 and 1129-2 g-2 might be the most tolerant in the design concentration range, followed by XJ968, and the weakest was 1128-2 p-2.
引文
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Ma Y.Q.,2011,Analysis on the situation of capsicum industry in China,Lajiao Zazhi(Journal of China Capsicum),(1):1-5(马艳青,2011,我国辣椒产业形势分析,辣椒杂志,(1):1-5)
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Pujari D.S.,and Chanda S.V.,2003,Salinity-induced changes in peroxidase activity and cell-wall polysaccharides in Vigna,Journal of Plant Biology,46(2):111-116
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Abdel Latef A.A.H.,Hashem A.,Rasool S.,Abd-Allah E.F.Alqarawi A.A.,Egamberdieva D.,Jan S.,Anjum N.A.,and Ahmad P.,2016,Arbuscular mycorrhizal symbiosis and abiotic stress in plants:a review,Journal Plant Biology,59(5)407-426
Cui X.C.,Zhang Q.,and Deng Z.R.,2005,Isoenzymes of capsicums in different development stages,Shanghai Daxue Xuebao(Journal of Shanghai University),11(6):645-651(崔西辰,张清,邓志瑞,2005,辣椒不同发育时期的同工酶研究,上海大学学报,11(6):645-651)
Deng Z.R.,and Luo L.,2009,Catalytic activity and isozyme patterns of superoxide dismutase in four species of capsicums under Cd2+stress,Shanghai Daxue Xuebao(Journal of Shanghai University),15(2):216-220(邓志瑞,罗琳,2009,镉离子胁迫下4种辣椒超氧化物歧化酶同工酶酶谱及酶活性分析,上海大学学报,15(2):216-220)
Geng S.S.,Chen B.,Zhang X.F.,and Du H.S.,2015,Breeding strategy and market demand trends of capsicum varieties in China,Zhongguo Shucai(China Vegetables),1(3):1-5(耿三省,陈斌,张晓芬,杜和山,2015,我国辣椒品种市场需求变化趋势及育种对策,中国蔬菜,1(3):1-5)
Guo X.,van Iersel M.W.,Chen J.R.,Brackett R.E.,and Beucha L.R.,2002,Evidence of association of salmonellae with tomato plants grown hydroponically in inoculated nutrien solution,Applied and Environmental Microbiology,68(7)3639-3643
Guzmán-Murillo M.A.,Ascencio F.,and Larrinaga-Mayoral J.A.2013,Germination and ROS detoxification in bell pepper(Capsicum annuum L.)under NaCl stress and treatment with microalgae extracts,Protoplasma,250(1):33-42
Li H.Y.,Yang X.Y.,Tang X.Q.,and Zhang H.X.,2016,Omics research progress of plants under salt stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),36(12)2548-2557(李焕勇,杨秀艳,唐晓倩,张华新,2016,植物响应盐胁迫组学研究进展,西北植物学报,36(12):2548-2557)
Li N.,Zhou B.L.,Bai L.P.,Fu Y.W.,and Huo S.F.,2006,The change of several protected enzyme's activity on grafted eggplants under salt stress,Liaoning Nongye Kexue(Liaoning Agricultural Sciences),(1):10-12(李宁,周宝利,白丽萍,付亚文,霍尚峰,2006,盐胁迫下嫁接茄子中几种保护酶活性的变化,辽宁农业科学,(1):10-12)
Li Y.,2008,Effects of salt stress on the photosynthesis and the antioxidant enzyme activity of tomato,Beifang Yuany(Northern Horticulture),(6):18-20(李妍,2008,盐胁迫对番茄幼苗生长及保护酶活性的影响,北方园艺,(6)18-20)
Lin C.C.,and Kao C.H.,1999,NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings,Plant and Soil,216(1):147-153
Ling Q.P.,Zeng Q.Y.,Hu F.,Chen J.W.,Wu J.Y.,Li Q.W.,and Qi Y.W.,2015,Molecular cloning and expression analysis of CIPK23 gene from sugarcane under low potassium,drought and salt stress,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(6):1329-1335(凌秋平,曾巧英,胡斐,陈健文,吴嘉云,李奇伟,齐永文,2015,甘蔗CIPK23基因克隆及对低钾、干旱、盐胁迫的响应,分子植物育种,13(6):1329-1335)
Liu J.C.,Li Y.H.,and Jin H.,2003,Effects of copper contamination on yields,accumulated copper distribution and membrane protective activities of pepper,Fujian Nongye Xuebao(Fujian Journal of Agricultural Sciences),18(4):254-257(刘景春,李裕红,晋宏,2003,铜污染对辣椒产量、铜累积及叶片膜保护酶活性的影响,福建农业学报,18(4):254-257)
Ma Y.Q.,2011,Analysis on the situation of capsicum industry in China,Lajiao Zazhi(Journal of China Capsicum),(1):1-5(马艳青,2011,我国辣椒产业形势分析,辣椒杂志,(1):1-5)
Meng X.P.,Yang H.S.,Guo H.,and Song C.Q.,2000,Effect of salt stress on peroxidase isoenzyme of winter wheat,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),15(4):62-67(孟学平,杨恒山,郭宏,宋彩琴,2000,盐胁迫对冬小麦过氧化物酶同工酶的影响,华北农学报,15(4):62-67)
Pei D.L.,Wang C.,and Li C.W.,2013,Study on POD enzyme activity and isozyme pattern analysis of peppers under low phosphorous stress,Beifang Yuanyi(Northern Horticulture),(23):113-116(裴冬丽,王冲,李成伟,2013,低磷胁迫下辣椒幼苗过氧化物酶活性及其同工酶谱的分析,北方园艺,(23):113-116)
Pujari D.S.,and Chanda S.V.,2003,Salinity-induced changes in peroxidase activity and cell-wall polysaccharides in Vigna,Journal of Plant Biology,46(2):111-116
Shi L.R.,and Zhang W.W.,2008.Effect of drought stress and salt stress on the isoperoxidases of wheat,Hengshui Xueyuan Xuebao(Journal of Hengshui University),10(4):50-52(时丽冉,张文文,2008,干旱和盐胁迫对小麦过氧化物同工酶的影响,衡水学院学报,10(4):50-52)
Song Y.,and Pei D.L.,2011,Effect of salt stress on peroxidase and isoenzyme in wheat seedlings,Hubei Nongye Kexue(Hubei Agricultural Sciences),50(9):1759-1761(宋旸,裴冬丽,2011,盐胁迫对小麦幼苗POD活性和同工酶的影响,湖北农业科学,50(9):1759-1761)
Sui Y.H.,and Hu N.B.,2015,Acquisition,identification and analysis of an interspecific Capsicum hybrid(C.annuum×C.chinense),Journal of Horticultural Science&Biotechnology,90(1):31-38
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