地木耳多糖热水提取工艺优化
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摘要
以地木耳为原料,采用单因素和响应面法优化了地木耳多糖的热水提取工艺,且进行脱蛋白和醇沉,得到地木耳多糖并对其理化性质进行测定。实验结果表明,响应面优化最佳工艺条件为料液比为1/55.62 g/mL,提取温度为366.37 K,提取时间为196.73 min,预测多糖得率为10.76%。实际提取条件修正为液比为1/50 g/mL,提取温度为370.15 K,提取时间为190 min,根据修正条件进行提取,多糖得率为10.43%。用硫酸钡比浊法测得地木耳多糖中硫酸根含量为2.18%;糖醛酸含量为33.41%,此地木耳多糖为酸性多糖。研究结果可为地木耳多糖提取工艺研究提供科学依据。
Nostoc commune was used as raw material, and the hot water extraction process of polysaccharide from Nostoc commune was optimized by using single factor and response surface method. After deproteinized and alcohol precipitation, the polysaccharides from Nostoc commune were obtained and their physicochemical properties were determined. The experimental results showed that the optimal reaction condition for response surface optimization was 1/55.62 g/mL of solid-liquid ratio, 366.37 K of extraction temperature, and 196.73 min of extraction time, and the predicted polysaccharide yield was 10.76%. The actual extraction conditions were corrected to the solid-liquid ratio of 1/50 g/mL, the extraction temperature of 370.15 K, and the extraction time of 190 min. According to the amended conditions for extraction, the polysaccharide yield was 10.43%. The content of sulfate radical in Nostoc commune polysaccharide was 2.18% with barium sulfate turbidimetry. The uronic acid content was 33.41%, indicating that the polysaccharide of this Nostoc commune was acidic polysaccharide. The results could provide scientific reference for the extraction of polysaccharide from Nostoc commune.
Nostoc commune was used as raw material, and the hot water extraction process of polysaccharide from Nostoc commune was optimized by using single factor and response surface method. After deproteinized and alcohol precipitation, the polysaccharides from Nostoc commune were obtained and their physicochemical properties were determined. The experimental results showed that the optimal reaction condition for response surface optimization was 1/55.62 g/mL of solid-liquid ratio, 366.37 K of extraction temperature, and 196.73 min of extraction time, and the predicted polysaccharide yield was 10.76%. The actual extraction conditions were corrected to the solid-liquid ratio of 1/50 g/mL, the extraction temperature of 370.15 K, and the extraction time of 190 min. According to the amended conditions for extraction, the polysaccharide yield was 10.43%. The content of sulfate radical in Nostoc commune polysaccharide was 2.18% with barium sulfate turbidimetry. The uronic acid content was 33.41%, indicating that the polysaccharide of this Nostoc commune was acidic polysaccharide. The results could provide scientific reference for the extraction of polysaccharide from Nostoc commune.
引文
Deconinck E.,Sokeng Djiogo C.A.,and Courselle P.,2017,Chemometrics and chromatographic fingerprints to classify plant food supplements according to the content of regulated plants,J.Pharm.Biomed.Anal.,143:48-55
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Fan Y.Q.,Wu X.Y.,and Yang L.Q.,2007,Study on extraction ofNostoc commune Vauch.polysaccharides by response surface methodology,Jiangsu Daxue Xuebao(Journal of Jiang su University),17(3):236-240(范群艳,吴向阳,仰榴青,2007,响应面分析法优化地木耳多糖提取工艺的研究,江苏大学学报,17(3):236-240)
Han P.P.,Yao S.Y.,Guo R.J.,Shen S.G.,Yan R.R.,Tan Z.L.,and Jia S.R.,2017,The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions,Carbohyd.Polym.,174:111-119
Huang H.D.,Wu M.M.,Yang H.P.,Li X.Y.,Ren M.N.,Li G.Q.,and Ma T.,2016,Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens,Carbohydr.Polym.,144:410-418
Ji W.,Tang N.,Zhao D.,and Zhang B.J.,2016,The pharmacological effects on Hericium erinaceus and its cultivation and application,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(5):1252-1257(纪伟,唐宁,赵端,张边江,2016,猴头菇的药理作用及栽培与应用,基因组学与应用生物学,35(5):1252-1257)
LI T.C.,Chen G.C.,and Suo Y.R.,2004,Characteristic analysis of amino acids of Nostoc commune in Qinghai Plateau,Anjisuan Yu Shengwu Ziyuan(Amino Acids and Biotic Resources),26(2):6-8(李天才,陈桂琛,索有瑞,2004,青海高原地木耳中氨基酸特征与分析,氨基酸与生物资源,26(2):6-8)
Mo K.J.,Xie B.J.,and Gong C.R.,2008,Effect of Nostoc sphaeroids Kutz polysaccharides on tumor inhibition and immunity of mice in vivo,Shipin Kexue(Food Science),29(4):392-396(莫开菊,谢笔钧,龚晨睿,2008,葛仙米多糖体内抑瘤及对免疫的影响,食品科学,29(4):392-396)
Nazifi E.,Wada N.,Asano T.,Nishiuchi T.,Iwamuro Y.,Chinaka S.,Matsugo S.,and Sakamoto T.,2015,Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune,J.Photoch.Photobiol.B.,142(3):154-168
Niu L.L.,Bi Y.,Zhang S.G.,and Liu L.,2010,Study on antimicrobial capability of different solvent extracts from Nostoc commune Vauch.,Shipin Gongye Keji(Science and technology of Food Industry),(1):68-70(牛黎莉,毕阳,张盛贵,刘磊,2010,地达菜中不同溶剂提取物抑菌能力的研究,食品工业科技,(1):68-70)
Qi G.L.,2015,Influence of meteorological factors on the appearance quality of Lycium barbarum L.and the accumu ution of medicinal composition of Lycium barbarum polysaccharides,Thesis for M.S.,Ningxia University,Supervisor:Wang J.,pp.18-21(齐国亮,2015,气象因子对宁夏枸杞外观品质及药用成分多糖积累的影响,硕士学位论文,宁夏大学,导师:王俊,pp.18-21)
Qiu F.P.,Zhang L.,and Yu J.,2005,Determination of the content of sulfate group in pilose antler polysaccarides by barium sulfate turbidity,Changchun Gongye Daxue Xuebao(Journal of Changchun University of Techonology),26(4):268-270(邱芳萍,张玲,于健,2005,硫酸钡比浊法对鹿茸多糖中硫酸基含量的测定,长春工业大学学报,26(4):268-270)
Tang J.,and Hu Z.Y.,2013,Antioxidant properties of polysaccharide extract of Nostoc commune,Shipin Yanjiu Yu Kaifa(Food Research and Development),34(9):1-4(汤俊,胡征宇,2013,地木耳多糖的抗氧化活性,食品研究与开发,34(9):1-4)
Wang J.,1995,A study on the influence of Nostoc commune on the content of nitrogen in soil,Lanzhou Daxue Xuebao(Journal of Lanzhou University),31(3):97-99(王静,1995,地木耳改变土壤含氮量的研究,兰州大学学报,31(3):97-99)
Wang S.,Lu A.X.,Zhang L.,Shen M.,Xu T.,Zhan W.Y.,Jin H.,Zhang Y.J.,and Wang W.M.,2017,Extraction and purification of pumpkin polysaccharides and their hypoglycemic effect,Int.J.Biol.Macromol.,98:182-187
Wang S.R.,Jiao S.T.,and Jiao G.S.,2013,Determination of nine elements in Nostoc connnune vanch by flame atomic absorp tion specteometry,Shipin Yanjiu Yu Kaifa(Food Research and Development),34(20):74-75(王淑荣,焦锁囤,焦更生,2013,FAAS法测定西北地木耳中9种元素的含量,食品研究与开发,34(20):74-75)
Wang Y.Y.,Wang T.J.,and Ding L.L.,2012,Optimization of polysaccharide extraction from Angelica sinensis using response surface methodology,Shipin Kexue(Food Science),33(10):146-149(王艳艳,王团结,丁琳琳,2012,响应面分析法优化当归多糖提取工艺,食品科学,33(10):146-149)
Zhang C.H.,Yun Y.H.,Zhang Z.M.,and Liang Y.Z.,2016,Simultaneous determination of neutral and uronic sugars based on UV-vis spectrometry combined with PLS,Int.J.Biol.Macromol.,87:290-294
Zhang T.W.,and Li T.C.,2011,Optimal extraction conditions on polysaccharide of Nostoc commune Vauch,Guangpu Shiyan shi(Chinese Journal of Spectroscopy Laboratory),28(1):144-148(张唐伟,李天才,2011,地木耳多糖的提取工艺研究,光谱实验室,28(1):144-148)
Zhang T.W.,Liu H.Q.,and Li T.C.,2011,Study on isolation and purification of polysaccharides extracted from Nostoc commune Vauch.,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),30(3):34-37(张唐伟,柳青海,李天才,2011,地木耳多糖的提取与纯化研究,中国野生植物资源,30(3):34-37)
Zhu C.P.,Zhai X.C.,Li L.Q.,Wu X.X.,and Li B.,2015,Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel,Food Chem.,177:139-146
Deng Z.Y.,Yan C.L.,Hu Q.,and Hu Z.Y.,2008,The research advance of Nostoc sphaeroides,Shuisheng Shengwu Xuebao(Acta Hydrobiologica Sinica),32(3):393-400(邓中洋,阎春兰,胡强,胡征宇,2008,葛仙米研究进展,水生生物学报,32(3):393-400)
Fan Y.Q.,Wu X.Y.,and Yang L.Q.,2007,Study on extraction ofNostoc commune Vauch.polysaccharides by response surface methodology,Jiangsu Daxue Xuebao(Journal of Jiang su University),17(3):236-240(范群艳,吴向阳,仰榴青,2007,响应面分析法优化地木耳多糖提取工艺的研究,江苏大学学报,17(3):236-240)
Han P.P.,Yao S.Y.,Guo R.J.,Shen S.G.,Yan R.R.,Tan Z.L.,and Jia S.R.,2017,The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions,Carbohyd.Polym.,174:111-119
Huang H.D.,Wu M.M.,Yang H.P.,Li X.Y.,Ren M.N.,Li G.Q.,and Ma T.,2016,Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens,Carbohydr.Polym.,144:410-418
Ji W.,Tang N.,Zhao D.,and Zhang B.J.,2016,The pharmacological effects on Hericium erinaceus and its cultivation and application,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(5):1252-1257(纪伟,唐宁,赵端,张边江,2016,猴头菇的药理作用及栽培与应用,基因组学与应用生物学,35(5):1252-1257)
LI T.C.,Chen G.C.,and Suo Y.R.,2004,Characteristic analysis of amino acids of Nostoc commune in Qinghai Plateau,Anjisuan Yu Shengwu Ziyuan(Amino Acids and Biotic Resources),26(2):6-8(李天才,陈桂琛,索有瑞,2004,青海高原地木耳中氨基酸特征与分析,氨基酸与生物资源,26(2):6-8)
Mo K.J.,Xie B.J.,and Gong C.R.,2008,Effect of Nostoc sphaeroids Kutz polysaccharides on tumor inhibition and immunity of mice in vivo,Shipin Kexue(Food Science),29(4):392-396(莫开菊,谢笔钧,龚晨睿,2008,葛仙米多糖体内抑瘤及对免疫的影响,食品科学,29(4):392-396)
Nazifi E.,Wada N.,Asano T.,Nishiuchi T.,Iwamuro Y.,Chinaka S.,Matsugo S.,and Sakamoto T.,2015,Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune,J.Photoch.Photobiol.B.,142(3):154-168
Niu L.L.,Bi Y.,Zhang S.G.,and Liu L.,2010,Study on antimicrobial capability of different solvent extracts from Nostoc commune Vauch.,Shipin Gongye Keji(Science and technology of Food Industry),(1):68-70(牛黎莉,毕阳,张盛贵,刘磊,2010,地达菜中不同溶剂提取物抑菌能力的研究,食品工业科技,(1):68-70)
Qi G.L.,2015,Influence of meteorological factors on the appearance quality of Lycium barbarum L.and the accumu ution of medicinal composition of Lycium barbarum polysaccharides,Thesis for M.S.,Ningxia University,Supervisor:Wang J.,pp.18-21(齐国亮,2015,气象因子对宁夏枸杞外观品质及药用成分多糖积累的影响,硕士学位论文,宁夏大学,导师:王俊,pp.18-21)
Qiu F.P.,Zhang L.,and Yu J.,2005,Determination of the content of sulfate group in pilose antler polysaccarides by barium sulfate turbidity,Changchun Gongye Daxue Xuebao(Journal of Changchun University of Techonology),26(4):268-270(邱芳萍,张玲,于健,2005,硫酸钡比浊法对鹿茸多糖中硫酸基含量的测定,长春工业大学学报,26(4):268-270)
Tang J.,and Hu Z.Y.,2013,Antioxidant properties of polysaccharide extract of Nostoc commune,Shipin Yanjiu Yu Kaifa(Food Research and Development),34(9):1-4(汤俊,胡征宇,2013,地木耳多糖的抗氧化活性,食品研究与开发,34(9):1-4)
Wang J.,1995,A study on the influence of Nostoc commune on the content of nitrogen in soil,Lanzhou Daxue Xuebao(Journal of Lanzhou University),31(3):97-99(王静,1995,地木耳改变土壤含氮量的研究,兰州大学学报,31(3):97-99)
Wang S.,Lu A.X.,Zhang L.,Shen M.,Xu T.,Zhan W.Y.,Jin H.,Zhang Y.J.,and Wang W.M.,2017,Extraction and purification of pumpkin polysaccharides and their hypoglycemic effect,Int.J.Biol.Macromol.,98:182-187
Wang S.R.,Jiao S.T.,and Jiao G.S.,2013,Determination of nine elements in Nostoc connnune vanch by flame atomic absorp tion specteometry,Shipin Yanjiu Yu Kaifa(Food Research and Development),34(20):74-75(王淑荣,焦锁囤,焦更生,2013,FAAS法测定西北地木耳中9种元素的含量,食品研究与开发,34(20):74-75)
Wang Y.Y.,Wang T.J.,and Ding L.L.,2012,Optimization of polysaccharide extraction from Angelica sinensis using response surface methodology,Shipin Kexue(Food Science),33(10):146-149(王艳艳,王团结,丁琳琳,2012,响应面分析法优化当归多糖提取工艺,食品科学,33(10):146-149)
Zhang C.H.,Yun Y.H.,Zhang Z.M.,and Liang Y.Z.,2016,Simultaneous determination of neutral and uronic sugars based on UV-vis spectrometry combined with PLS,Int.J.Biol.Macromol.,87:290-294
Zhang T.W.,and Li T.C.,2011,Optimal extraction conditions on polysaccharide of Nostoc commune Vauch,Guangpu Shiyan shi(Chinese Journal of Spectroscopy Laboratory),28(1):144-148(张唐伟,李天才,2011,地木耳多糖的提取工艺研究,光谱实验室,28(1):144-148)
Zhang T.W.,Liu H.Q.,and Li T.C.,2011,Study on isolation and purification of polysaccharides extracted from Nostoc commune Vauch.,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),30(3):34-37(张唐伟,柳青海,李天才,2011,地木耳多糖的提取与纯化研究,中国野生植物资源,30(3):34-37)
Zhu C.P.,Zhai X.C.,Li L.Q.,Wu X.X.,and Li B.,2015,Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel,Food Chem.,177:139-146