均匀设计法优选银花清咽纳米乳喷雾剂提取工艺
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摘要
目的:优选银花清咽纳米乳喷雾剂的提取工艺。方法:以绿原酸、木犀草苷、甘草酸铵、桔梗皂苷D和总黄酮含量为评价指标,用U6(64)均匀设计表安排试验,考察溶剂倍数、浸泡时间、提取时间和提取次数4个因素对提取工艺的影响,综合加权评分,对数据进行拟合得到回归方程,优选最优工艺。结果:最优工艺为12倍水,浸泡0. 5 h,提取3次,每次1 h。结论:试验设计合理,优选的提取工艺稳定、可操作性强,可作为银花清咽纳米乳喷雾剂的提取工艺,为该制剂后续药效学评价提供依据。
Objective: To optimize the extraction process of Yinhua Qingyan nanoemulsion spray. Methods: With chlorogenic acid,cynarosid,glycyrrhizic acid ammonium salt,platycodin D and total flavone as the evaluation indices,U6(64) uniform design table was used to arrange the experiments with the solvent ratio,soaking time,extracting time and extracting times as the influencing factors. The comprehensive weighted score was calculated,and the data was fit to obtain the regression equation in order to optimize the extraction process. Results: The optimal process was 12-fold amount of water,soaking 0. 5 h and extracting 3times with 1 h for each time. Conclusion: The experimental design is reasonable,and the optimized extraction process is stable and operable,which can be used as the extraction process of Yinhua Qingyan nanoemulsion spray. The study provides basis for the subsequent pharmacodynamics evaluation of the preparation.
Objective: To optimize the extraction process of Yinhua Qingyan nanoemulsion spray. Methods: With chlorogenic acid,cynarosid,glycyrrhizic acid ammonium salt,platycodin D and total flavone as the evaluation indices,U6(64) uniform design table was used to arrange the experiments with the solvent ratio,soaking time,extracting time and extracting times as the influencing factors. The comprehensive weighted score was calculated,and the data was fit to obtain the regression equation in order to optimize the extraction process. Results: The optimal process was 12-fold amount of water,soaking 0. 5 h and extracting 3times with 1 h for each time. Conclusion: The experimental design is reasonable,and the optimized extraction process is stable and operable,which can be used as the extraction process of Yinhua Qingyan nanoemulsion spray. The study provides basis for the subsequent pharmacodynamics evaluation of the preparation.
引文
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2王婷,娄鑫,苗明三.代代花的现代研究与思考[J].中医学报,2017,2(32):276-278
3李盈,王举涛,桂双英,等.桔梗的化学成分及药理作用研究进展[J].食品与药品,2016,18(1):72-75
4田志伟.红花药理分析及临床应用研究[J].临床医药文献电子杂志,2017,4(80):15833
5蒋丽丽,张彦龙,王春杰,等.牡丹皮中有效成分丹皮酚的药理活性研究进展[J].现代诊断与治疗,2016,27(22):4223-4244
6周劭宣,刘玥欣,黄晓巍,鲍慧玮,杨波.药食两用中药玉竹药理作用及其应用的研究进展[J].人参研究,2017,29(03):52-54
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8刘长河,王艳艳,陈杰,等.金银花中有效成分的含量与其质量等级相关性研究[J].辽宁中医杂志,2016,43(11):2352-2354
9蒲忠慧,陈希文,赵顺娇,等.均匀设计法优选红景天多糖提取工艺[J].绵阳师范学院学报,2017,36(8):84-88
10孙丽娜,刘小虹,刘建博,等.复方五指毛桃颗粒的提取工艺研究[J].中国药房,2017,28(28):3976-3979
11秦明明,陈奕,胡子燕.清咽含片的制备工艺[J].医药导报,2010,29(10):1340-1341
12徐双枝,张婷,高家荣,等.多指标综合加权评分法优选补骨脂-肉豆蔻药对水提工艺研究[J].中国医院药学杂志,2016,36(18):1558-1563
13王京龙,郑丹丹,王磊,等.均匀设计法优化葛根半仿生提取工艺[J].中成药,2017,35(6):1084-1087