铜对奶牛免疫能力的影响
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摘要
[目的]检测实验奶牛血清中的微量元素铜的水平及相关指标的变化规律。[方法]实验所用实验动物均选自黑龙江地区某奶牛场,常规水平饲养管理,TMR全混合日粮自由采食。根据DHI数据随机选择体重、胎次(2～3次)、泌乳期(0～10 d)、体况和饲养管理高度相似的泌乳奶牛50头。[结果]实验奶牛血清铜水平与IL-2的相关性为R=0.355,显著性为P=0.011。实验奶牛血清铜水平与IL-2呈显著正相关,泌乳奶牛血清中的IL-2会随着铜水平的升高而有所升高。实验奶牛血清铜水平与IL-1的相关性为R=0.215,显著性为Ρ=0.133,P>0.05。实验奶牛血清铜水平与IL-1不相关。实验奶牛血清铜水平与TNF-α的相关性为R=0.185,显著性为P=0.198,P>0.05。[结论]实验奶牛血清铜水平与TNF-α不相关。
[Objective]To detect the serum level of trace element of copper in experimental dairy cows and to characterize the variation rule of related indexes. [Method]The experimental dairy cows used in this study were selected from a dairy farm in Heilongjiang Province, and were reared under free-choice feeding system with TMR diets. According to the DHI data, 50 lactating cows with similar body weight, parity(2-3 times), lactation period(0-10 days), body condition and feeding management were randomly selected. [Result]The correlation between serum copper level and IL-2 content in the experimental dairy cows was R=0.355, P=0.011. There was a significantly positive correlation between serum copper level and IL-2 content in the experimental dairy cows. The serum content of IL-2 in the lactating dairy cows was increased with the elevation of serum copper level. The correlation between serum copper level and IL-1 content in the experimental dairy cows was R=0.215, and the significance was P=0.133, P>0.05. There was no correlation between serum copper level and IL-1 content in the experimental dairy cows. The correlation between serum copper level and TNF-alpha content in the experimental dairy cows was R=0.185,with a significant difference of P=0.198, P>0.05. [Conclusion]There was no correlation between serum copper level and TNF-alpha content in experimental dairy cows.
[Objective]To detect the serum level of trace element of copper in experimental dairy cows and to characterize the variation rule of related indexes. [Method]The experimental dairy cows used in this study were selected from a dairy farm in Heilongjiang Province, and were reared under free-choice feeding system with TMR diets. According to the DHI data, 50 lactating cows with similar body weight, parity(2-3 times), lactation period(0-10 days), body condition and feeding management were randomly selected. [Result]The correlation between serum copper level and IL-2 content in the experimental dairy cows was R=0.355, P=0.011. There was a significantly positive correlation between serum copper level and IL-2 content in the experimental dairy cows. The serum content of IL-2 in the lactating dairy cows was increased with the elevation of serum copper level. The correlation between serum copper level and IL-1 content in the experimental dairy cows was R=0.215, and the significance was P=0.133, P>0.05. There was no correlation between serum copper level and IL-1 content in the experimental dairy cows. The correlation between serum copper level and TNF-alpha content in the experimental dairy cows was R=0.185,with a significant difference of P=0.198, P>0.05. [Conclusion]There was no correlation between serum copper level and TNF-alpha content in experimental dairy cows.
引文
[1]BROADLEY C,HOOVER R L.Ceruloplasmin reduces the adhesion and scavenges superoxide during the interaction of activated polymorphonuclear leukocytes with endothelial cells[J].American Journal of Pathology,1989,135:647-655.
[2]SPEARS J W.Trace mineral bioavailability in ruminants[J].Journal of Nutrition,2003,133:1506-1509.
[3]许楚楚,刘健男,夏成,等.泌乳奶牛铜缺乏与机体氧化应激的关系[J].中国兽医杂志,2015,51(7):27-29.
[4]SUTTLE N F,JONES D G.Copper and disease resistance in sheep:a rare natural confirmation of interaction between a specific nutrient and infection[J].Proceedings of the Nutrition Society,1986,45:317-325.
[5]SPEARS J W,KEGLEY E B.Effect of zinc source(zinc oxide vs.zinc proteinate)and level on performance,carcass characteristics,and immune response of growing and finishing steers[J].Journal of Animal Science,2002,80:2747-2752.
[6]TORRE P M,HARMON R J,SORDILLO L M,et al.Modulation of bovine mononuclear cell proliferation and cytokine production by dietary copper insufficiency[J].Journal of Nutritional Immunology,1995(3):3-20.
[7]TORRE P M,HARMON R J,HEMKEN R W,et al.Mild dietary copper insufficiency depresses blood neutrophil function in diary cattle[J].Journal of Nutritional Immunology,1996(4):3-24.
[8]HARMON R J.Trace minerals and dairy cattle:importance for udder health[C]//LYONS T P,JACQUES K A.Biotechnology in the Feed Industry,Proceedings of Alltech′s 14thAnnual Symposium.Nottingham:Nottingham University Press,1998:485-495.
[9]SCALETTI R W,TRAMMEL D S,SMITH B A,et al.Role of dietary copper in enhancing resistance to Escherichia coli mastitis[J].Journal of Dairy Science,2003,86:1240-1249.
[10]STABEL J R,SPEARS J W,BROWN T T,et al.Selenium effects on glutathione peroxidase and the immune response of stressed calves challenged with Pasteurella hemolytica[J].Journal of Animal Science,1989,67:557-564.
[11]WARD J D,GENGELBACH G P,SPEARS J W.The effects of copper deficiency with or without high dietary iron or molybdenum on immune function of cattle[J].Journal of Animal Science,1997,75:1400-1408.
[12]李红霞,张娜,张正.妊娠妇女常量元素和必需微量元素的含量及分析[J].中国妇产科临床杂志,2006,17(6):433-443.
[13]崔恒敏.铜对免疫功能影响的研究进展[J].动物科学与动物医学,2004,21(11):37-39.
[14]BABU U,FAILLA M L.Respiratory burst and candidacidal activity of peritoneal macrophages are impaired in copper-deficient rats[J].Journal of Nutrition,1990,120(12):1692.
[15]金虹.小鼠缺铜对腹腔巨噬细胞产生肿瘤坏死因子和白介素的影响[J].营养学报,1996,18(3):300-303.
[16]HOPKINS S G,FAILLA M L.Chronic intake of a marginally low copperdiet impair in vitro of lymphocytes and meutrophiles from male ratsdespite minimal impact on conventional indicators of copper status[J].Journal of Nutrition,1995,125(10):2658-2668.
[17]HARMON R J,CLARK T W,TRAMMELL D S,et al.Influence of copper status in heifers on response to intramammary challenge with Escherichia coli endotoxin[J].Dairy Sci,1994,77(1):198.
[2]SPEARS J W.Trace mineral bioavailability in ruminants[J].Journal of Nutrition,2003,133:1506-1509.
[3]许楚楚,刘健男,夏成,等.泌乳奶牛铜缺乏与机体氧化应激的关系[J].中国兽医杂志,2015,51(7):27-29.
[4]SUTTLE N F,JONES D G.Copper and disease resistance in sheep:a rare natural confirmation of interaction between a specific nutrient and infection[J].Proceedings of the Nutrition Society,1986,45:317-325.
[5]SPEARS J W,KEGLEY E B.Effect of zinc source(zinc oxide vs.zinc proteinate)and level on performance,carcass characteristics,and immune response of growing and finishing steers[J].Journal of Animal Science,2002,80:2747-2752.
[6]TORRE P M,HARMON R J,SORDILLO L M,et al.Modulation of bovine mononuclear cell proliferation and cytokine production by dietary copper insufficiency[J].Journal of Nutritional Immunology,1995(3):3-20.
[7]TORRE P M,HARMON R J,HEMKEN R W,et al.Mild dietary copper insufficiency depresses blood neutrophil function in diary cattle[J].Journal of Nutritional Immunology,1996(4):3-24.
[8]HARMON R J.Trace minerals and dairy cattle:importance for udder health[C]//LYONS T P,JACQUES K A.Biotechnology in the Feed Industry,Proceedings of Alltech′s 14thAnnual Symposium.Nottingham:Nottingham University Press,1998:485-495.
[9]SCALETTI R W,TRAMMEL D S,SMITH B A,et al.Role of dietary copper in enhancing resistance to Escherichia coli mastitis[J].Journal of Dairy Science,2003,86:1240-1249.
[10]STABEL J R,SPEARS J W,BROWN T T,et al.Selenium effects on glutathione peroxidase and the immune response of stressed calves challenged with Pasteurella hemolytica[J].Journal of Animal Science,1989,67:557-564.
[11]WARD J D,GENGELBACH G P,SPEARS J W.The effects of copper deficiency with or without high dietary iron or molybdenum on immune function of cattle[J].Journal of Animal Science,1997,75:1400-1408.
[12]李红霞,张娜,张正.妊娠妇女常量元素和必需微量元素的含量及分析[J].中国妇产科临床杂志,2006,17(6):433-443.
[13]崔恒敏.铜对免疫功能影响的研究进展[J].动物科学与动物医学,2004,21(11):37-39.
[14]BABU U,FAILLA M L.Respiratory burst and candidacidal activity of peritoneal macrophages are impaired in copper-deficient rats[J].Journal of Nutrition,1990,120(12):1692.
[15]金虹.小鼠缺铜对腹腔巨噬细胞产生肿瘤坏死因子和白介素的影响[J].营养学报,1996,18(3):300-303.
[16]HOPKINS S G,FAILLA M L.Chronic intake of a marginally low copperdiet impair in vitro of lymphocytes and meutrophiles from male ratsdespite minimal impact on conventional indicators of copper status[J].Journal of Nutrition,1995,125(10):2658-2668.
[17]HARMON R J,CLARK T W,TRAMMELL D S,et al.Influence of copper status in heifers on response to intramammary challenge with Escherichia coli endotoxin[J].Dairy Sci,1994,77(1):198.