甜瓜种质资源芽苗期和幼苗期耐NO_3~-性评价及鉴定指标筛选
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摘要
为筛选出耐盐性甜瓜材料和提供早期鉴定指标,利用150 mmol/L NO_3~-对23份甜瓜种质芽苗期和幼苗期进行胁迫处理,以多项指标盐害系数隶属函数值和总隶属函数值为依据,比较了2个时期不同种质的耐盐性,并进行了耐盐性鉴定指标的筛选。结果表明,不同甜瓜种质对NO_3~-胁迫处理的响应有明显差异,芽苗期和幼苗期耐盐性材料有4份相同,分别占芽苗期和幼苗期耐盐材料的57.14%和25.00%,耐盐和中等耐盐材料相同率为90.00%;并筛选出胚根干质量、主胚根长可作为芽苗期耐盐性鉴定指标,茎粗、根干质量、壮苗指数、叶绿素a+b/类胡萝卜素、地上部干质量可作为幼苗期耐盐性鉴定指标。
In order to screen salt-tolerant materials and provide early identification indices of melon,150 mmol/L NO_3~- solution was used to treat 23 melon germplasms at sprout and seedling stages,and based on subordinate function values and total subordinate function value of salt damage coefficient,the salt tolerance was compared and the salt tolerance identification indexes were screened. The result showed that the responses of different melon germplasms to NO_3~- stress treatment were obviously different and salt tolerance at sprout and seedling stages was varied. Four salt tolerant materials were the same,which occupied 57. 14% and 25. 00% of salt tolerant materials at sprout stages and seedling stages respectively,and the same rate of salt tolerant and medium salt tolerant materials was 90. 00%. Radicle dry weight and main radicle length were screened as salt resistance identification indexes for melon at sprout stages,and stem diameter,root dry weight,strong seedling index,chlorophyll a + b/carotenoids and above ground dry weight could be used as salt resistance appraisal indicators for melon at seedling stage.
In order to screen salt-tolerant materials and provide early identification indices of melon,150 mmol/L NO_3~- solution was used to treat 23 melon germplasms at sprout and seedling stages,and based on subordinate function values and total subordinate function value of salt damage coefficient,the salt tolerance was compared and the salt tolerance identification indexes were screened. The result showed that the responses of different melon germplasms to NO_3~- stress treatment were obviously different and salt tolerance at sprout and seedling stages was varied. Four salt tolerant materials were the same,which occupied 57. 14% and 25. 00% of salt tolerant materials at sprout stages and seedling stages respectively,and the same rate of salt tolerant and medium salt tolerant materials was 90. 00%. Radicle dry weight and main radicle length were screened as salt resistance identification indexes for melon at sprout stages,and stem diameter,root dry weight,strong seedling index,chlorophyll a + b/carotenoids and above ground dry weight could be used as salt resistance appraisal indicators for melon at seedling stage.
引文
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[26]程智慧.番茄和黄瓜耐盐鉴定及盐逆境对蔗糖代谢和离子吸收的影响[D].杨凌:西北农林科技大学,2008.
[27]阮松林,薛庆中.盐胁迫条件下杂交水稻种子发芽特性和幼苗耐盐生理基础[J].中国水稻科学,2002,16(3):281-284.
[28]孙凯,张胜利,朱弘博.利用隶属函数法对不同基因型甘薯耐盐碱能力的分析与评价[J].东北师范大学学报(自然科学版),2015,47(2):115-119.
[2]黄易,张玉龙.保护地生产条件下的土壤退化问题及其防治对策[J].土壤通报,2004,35(2):212-216.
[3]王述民,李立会,黎裕,等.中国粮食和农业植物遗传资源状况报告(Ⅰ)[J].植物遗传资源学报,2011,12(2):167-177.
[4]中华人民共和国农业部.中国农业统计资料(2015)[M].北京:中国农业出版社,2016.
[5]张巧凤,陈宗金,吴纪中,等.小麦种质芽期和苗期的耐盐性鉴定评价[J].植物遗传资源学报,2013,14(4):620-626.
[6]张国伟,路海玲,张雷,等.棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J].应用生态学报,2011,22(8):2045-2053.
[7]张国伟,路海玲,周治国.棉花萌发期和苗期耐盐性及耐盐指标评价[C]//中国棉花学会.2010年年会论文汇编.郑州:中国棉花学会,2010:243-250.
[8]董志刚,程智慧.番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3):1348-1355.
[9]董志刚,孟焕文,程智慧.黄瓜品种资源芽苗期和幼苗期耐盐性及其评价指标研究[J].干旱地区农业研究,2008,26(4):156-162.
[10]姚金晓,朱家骝,杨飞,等.海水胁迫下冬瓜幼芽期和幼苗期耐盐性评价及耐盐指标筛选[J].西北农业学报,2016,25(4):612-618.
[11]朱春燕.甜瓜品种资源耐盐性及其指标评价[D].上海:上海交通大学,2011.
[12]童有为,陈淡飞.温室土壤次生盐渍化的形成和治理途径研究[J].园艺学报,1991,18(2):159-162.
[13]曾希柏,白玲玉,苏世鸣,等.山东寿光不同种植年限设施土壤的酸化与盐渍化[J].生态学报,2010,30(7):1853-1859.
[14]魏国强,朱祝军,方学智,等.Na Cl胁迫对不同品种黄瓜幼苗生长、叶绿素荧光特性和活性氧代谢的影响[J].中国农业科学,2004,37(11):1754-1759.
[15]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[16]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2001.
[17]陈德明,俞仁培,杨劲松.盐渍条件下小麦抗盐性的隶属函数值法评价[J].土壤学报,2002,39(3):368-373.
[18]吴兰荣,陈静,许婷婷,等.花生全生育期耐盐鉴定研究[J].花生学报,2005,34(1):20-24.
[19]盖如玉.大豆种质资源的耐盐性鉴定和多样性分析[D].北京:中国农业科学院,2007.
[20]王东明,贾媛,崔继哲.盐胁迫对植物的影响及植物盐适应性研究进展[J].中国农学通报,2009,25(4):124-128.
[21]徐慧妮,王秀峰,孙旭东,等.黄瓜种子萌发对NO-3胁迫的响应及耐盐性评价[J].西北植物学报,2011,31(2):325-331.
[22]陈火英,李怀志,刘杨,等.番茄耐盐性及耐盐育种研究进展[J].上海交通大学学报(农业科学版),2007,25(5):507-512.
[23]Merchan F,De Lorenzo L,Rizzo S G,et al.Identification of regulatory pathways involved in the reacquisition of root growth after salt stress in Medicago truncatula[J].Plant J,2007,51(1):1-17.
[24]Hodge A,Berta G,Doussan C,et al.Plant root growth,architecture and function[J].Plant Soil,2009,321(1):153-187.
[25]曹齐卫,李利斌,孔素萍,等.不同黄瓜品种幼苗对等渗Mg(NO3)2和Na Cl胁迫的生理响应[J].应用生态学报,2015,26(4):1171-1178.
[26]程智慧.番茄和黄瓜耐盐鉴定及盐逆境对蔗糖代谢和离子吸收的影响[D].杨凌:西北农林科技大学,2008.
[27]阮松林,薛庆中.盐胁迫条件下杂交水稻种子发芽特性和幼苗耐盐生理基础[J].中国水稻科学,2002,16(3):281-284.
[28]孙凯,张胜利,朱弘博.利用隶属函数法对不同基因型甘薯耐盐碱能力的分析与评价[J].东北师范大学学报(自然科学版),2015,47(2):115-119.