三种质量浓度医用臭氧局部注射对兔膝骨性关节炎行为学及滑膜匀浆中IL-1、TNF-α水平的影响
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摘要
目的探讨三种质量浓度臭氧对新西兰兔膝骨性关节炎行为学及血清中致炎因子IL-1、TNF-α水平的影响。方法随机将90只新西兰兔分成5组,分别为正常组、模型组、臭氧20μg/ml组、臭氧30μg/ml组、臭氧50μg/ml组,正常组不进行任何处理,模型组仅造模,其余3组造模之后注射3 ml的臭氧,臭氧治疗1 d、5 d和10 d后,观察各组新西兰兔Lequesne MG膝关节评估级别及滑膜匀浆中IL-1、TNF-α的水平变化情况。结果 Lequesne MG膝关节评估在局部反应、步态反应、关节活动和关节肿胀方面各个臭氧治疗组与模型组、正常组相比差异有统计学意义(P<0.05);治疗1 d、5 d、10 d后,IL-1及TNF-α两种因子的质量浓度变化一致,均为臭氧50μg/ml组>模型组>臭氧30μg/ml组>臭氧20μg/ml组>正常组;臭氧20μg/ml组、臭氧30μg/ml组的IL-1、TNF-α的质量浓度均显著降低(均P<0.05);臭氧治疗10 d后,臭氧20μg/ml组兔滑膜匀浆中IL-1、TNF-α质量浓度与正常组相比差异不显著(P>0.05);臭氧50μg/ml组的IL-1、TNF-α的质量浓度高于模型组(均P<0.05)。结论臭氧浓度为20、30μg/ml时可以显著改善新西兰兔行为学功能,降低新西兰兔膝关节损伤部位滑膜匀浆中IL-1、TNF-α的质量浓度,延缓KOA病程的进展;当臭氧浓度为50μg/ml时加重了新西兰兔骨关节的损伤。
Objective To investigate the effects of three concentrations of ozone on the expressions of IL-1 and TNF-αin osteoblastic arthritis of New Zealand rabbits. Methods Ninety New Zealand white rabbits were randomly divided into five groups:normal group,model group,20 μg/ml ozone group,30 μg/ml ozone group and 50 μg/ml ozone group. The last three groups were injected with 3 m L of ozone. After ozone treatment for 1 d,5 d and 10 d,the changes of Lequesne MG knee joint grade evaluation and levels of IL-1 and TNF-α in synovial homogenates in each group were observed. Results There was significant difference between the model group and normal group in local reactions,gait reaction,joint activity and joint swelling( P<0. 05) according to Lequesne MG knee evaluation. After 1 d,5 d and 10 d,the concentrations of IL-1 and TNF-αin the two groups changed in the same way: ozone 50μg/ml group>model group>ozone 30 μg/ml group>ozone 20 μg/ml group>normal group. The expressions of IL-1 and TNF-αin ozone 20 μg/ml group and 30 μg/ml ozone group were significantly decreased( all P<0. 05). After 10 days of ozone treatment,the concentrations of IL-1 and TNF-αin rabbit synovial homogenates of the 20 μg/ml ozone group were significantly decreased. Compared with the normal group,the difference was not significant( P >0. 05). The concentrations of IL-1 and TNF-α in the 50 μg/ml ozone group was higher than that in the model group( all P<0. 05). Conclusion Ozone at the concentrations of 20 μg/ml and 30 μg/ml can significantly improve the behavioral function of New Zealand rabbits,reduce the concentration of IL-1 and TNF-αin their synovial homogenates,delay the progression of KOA. However,ozone at a concentration of50 μg/ml can aggravate bone and joint damage of New Zealand rabbits.
Objective To investigate the effects of three concentrations of ozone on the expressions of IL-1 and TNF-αin osteoblastic arthritis of New Zealand rabbits. Methods Ninety New Zealand white rabbits were randomly divided into five groups:normal group,model group,20 μg/ml ozone group,30 μg/ml ozone group and 50 μg/ml ozone group. The last three groups were injected with 3 m L of ozone. After ozone treatment for 1 d,5 d and 10 d,the changes of Lequesne MG knee joint grade evaluation and levels of IL-1 and TNF-α in synovial homogenates in each group were observed. Results There was significant difference between the model group and normal group in local reactions,gait reaction,joint activity and joint swelling( P<0. 05) according to Lequesne MG knee evaluation. After 1 d,5 d and 10 d,the concentrations of IL-1 and TNF-αin the two groups changed in the same way: ozone 50μg/ml group>model group>ozone 30 μg/ml group>ozone 20 μg/ml group>normal group. The expressions of IL-1 and TNF-αin ozone 20 μg/ml group and 30 μg/ml ozone group were significantly decreased( all P<0. 05). After 10 days of ozone treatment,the concentrations of IL-1 and TNF-αin rabbit synovial homogenates of the 20 μg/ml ozone group were significantly decreased. Compared with the normal group,the difference was not significant( P >0. 05). The concentrations of IL-1 and TNF-α in the 50 μg/ml ozone group was higher than that in the model group( all P<0. 05). Conclusion Ozone at the concentrations of 20 μg/ml and 30 μg/ml can significantly improve the behavioral function of New Zealand rabbits,reduce the concentration of IL-1 and TNF-αin their synovial homogenates,delay the progression of KOA. However,ozone at a concentration of50 μg/ml can aggravate bone and joint damage of New Zealand rabbits.
引文
[1]李敏,袁桂平.内热针联合臭氧穴位注射介入治疗新西兰兔膝骨关节炎的实验研究[J].中国中医急症,2016,25(10):1866.
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[5]XIANG Y,MASUKO-HONGO K,SEKINE T,et al.Expression of proteinaseactivated receptors(PAR)-2 in articular chondrocytes is modulated by IL-1beta,TNF-alpha and TGF-beta[J].Osteoarthritis Cartilage,2006,14(11):1163.
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[7]薛涛,杨延庆,李晓莉,等.Hcy、IL-1β和TNF-α水平与初发脑梗死患者抑郁程度的相关性研究[J].国际精神病学杂志,2015,42(6):13.
[8]邹晓萍,董化江,单娜娜,等.蕨麻正丁醇提取物对急性卵巢缺血再灌注损伤大鼠肿瘤坏死因子-α的影响[J].新乡医学院学报,2014,31(5):321.
[9]姚雪萍,张建龙,龙梅,等.黑木耳提取物对脓毒血症大鼠细胞因子TNF-α、IL-1的影响[J].新疆医科大学学报,2012,35(4):472.
[10]中华医学会呼吸病学分会慢性阻塞性肺疾病学组.慢性阻塞性肺疾病诊治指南(2013年修订版)[J].中华结核和呼吸杂志,2013,36(4):255.
[11]蔡柏蔷.呼吸内科学[M].北京:中国协和医科大学出版社,2000:480.
[12]LABIB S,WAGIH K,WAGIH Y.Evaluation of antiendothelial cell antibodies in COPD patients,with and without corpulmonale[J].Egyptian Journal of Chest Diseases and Tuberculosis,2014,63(3):125.
[13]赵东芳.川芎嗪注射液联合红景天注射液治疗慢性阻塞性肺疾病合并肺动脉高压疗效观察[J].现代中西医结合杂志,2015,24(31):3435.
[2]李蔚勃,曹佩华,曹峻岭.α-2巨球蛋白对骨关节炎的影响[J].国外医学(医学地理分册),2006(1):1.
[3]杨晓霞,杨衍涛,马莉,等.BNP、CRP、TNF-α、IL-6与慢性充血性心力衰竭的诊断和心功能分级相关性[J].兰州大学学报(医学版),2012,38(2):40.
[4]王燕林,梁文妹,李容,等.某54度食用白酒对大鼠肝细胞TNF-α表达及凋亡的影响[J].贵州医科大学学报,2017,42(6):661.
[5]XIANG Y,MASUKO-HONGO K,SEKINE T,et al.Expression of proteinaseactivated receptors(PAR)-2 in articular chondrocytes is modulated by IL-1beta,TNF-alpha and TGF-beta[J].Osteoarthritis Cartilage,2006,14(11):1163.
[6]陈序,李琦.七氟醚预处理对大鼠肝脏缺血再灌注损伤后TNF-α、IL-1β的影响[J].广西医科大学学报,2012,29(6):841.
[7]薛涛,杨延庆,李晓莉,等.Hcy、IL-1β和TNF-α水平与初发脑梗死患者抑郁程度的相关性研究[J].国际精神病学杂志,2015,42(6):13.
[8]邹晓萍,董化江,单娜娜,等.蕨麻正丁醇提取物对急性卵巢缺血再灌注损伤大鼠肿瘤坏死因子-α的影响[J].新乡医学院学报,2014,31(5):321.
[9]姚雪萍,张建龙,龙梅,等.黑木耳提取物对脓毒血症大鼠细胞因子TNF-α、IL-1的影响[J].新疆医科大学学报,2012,35(4):472.
[10]中华医学会呼吸病学分会慢性阻塞性肺疾病学组.慢性阻塞性肺疾病诊治指南(2013年修订版)[J].中华结核和呼吸杂志,2013,36(4):255.
[11]蔡柏蔷.呼吸内科学[M].北京:中国协和医科大学出版社,2000:480.
[12]LABIB S,WAGIH K,WAGIH Y.Evaluation of antiendothelial cell antibodies in COPD patients,with and without corpulmonale[J].Egyptian Journal of Chest Diseases and Tuberculosis,2014,63(3):125.
[13]赵东芳.川芎嗪注射液联合红景天注射液治疗慢性阻塞性肺疾病合并肺动脉高压疗效观察[J].现代中西医结合杂志,2015,24(31):3435.