力龙康复合木耳粉止咳祛痰作用研究
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摘要
为探讨复合木耳粉的止咳、祛痰作用,本研究应用中药清肺润肺动物模型,采用浓氨水引咳和酚红排泌量实验。将ICR小鼠随机分为5组,分别为空白对照组(等容生理盐水),阳性对照组(川贝枇杷露),复合木耳粉低(0. 05 g/m L)、中(0. 1 g/m L)、高(0. 3g/m L)剂量组。每组10只,灌胃体积为25 m L/kg。阳性对照川贝枇杷膏依据成人与小鼠体积系数换算,小鼠等效剂量为5. 85 m L/kg,吸取5. 85 m L原液,加入生理盐水稀释至50 m L,连续灌胃30 d,2次/d,最后一次灌胃后进行氨水引咳实验和酚红排泌量测定试验。氨水引咳法实验小鼠灌胃30 d后,观察小鼠咳嗽潜伏期及3 min内咳嗽次数;酚红排泌实验小鼠灌胃30 d,于末次灌胃1 h后腹腔注射5%酚红溶液,观察小鼠气管酚红排泌量。与正常对照组比较,阳性对照组、复合木耳粉各剂量组均能延长浓氨水所致小鼠咳嗽的潜伏期(P <0. 01);均能减少浓氨水所致小鼠咳嗽次数(P <0. 01);并增加小鼠气管酚红排泌量(P <0. 01)。与阳性对照组比较,复合木耳粉高剂量组引咳潜伏期延长,3 min内咳嗽次数减少,止咳效果作用与阳性对照组相当;复合木耳粉中、高剂量组酚红排泌量减少,组间无差异,作用效果稍低于阳性对照组。说明,复合木耳粉具有止咳、祛痰作用。
To investigate the effects of compound auricularia powder on relieving cough and expectorant. ICR mice were randomly divided into five groups: blank control group( isovolumic saline),positive control group( Chuanbei loquat dew),low( 0. 05 g/m L),medium( 0. 1 g/m L) and high( 0. 3 g/m L) dosage group. Ten rats in each group were given intragastric administration of 25 m L/kg. The equivalent dose of Chuanbei loquat ointment was 5. 85 m L/kg,the original solution was absorbed 5. 85 m L,diluted to 50 m L with normal saline,and then gavaged for 30 days,twice a day. After the last gavage,the cough induced by ammonia water and the phenol red excretion were measured. The cough latency and the number of coughs within 3 minutes were observed after 30 days of oral administration of ammonia water. The phenol red excretion test mice were given orally for 30 days and intraperitoneally injected with 5% phenol red solution 1 hour after the last oral administration to observe the amount of phenol red excretion in trachea of mice.Compared with the normal control group,the incubation period of cough induced by concentrated ammonia could be prolonged in both the positive control group and the composite auricle powder group( P < 0. 01); The cough frequency of mice induced by concentrated ammonia could be reduced( P < 0. 01); The amount of phenol red excretion from trachea of mice was increased( P < 0. 01). Compared with the positive control group,the incubation period of cough induced in the high dose group of compound auricularia powder was prolonged,the number of coughs within 3 min was reduced,and the effect of cough relief was similar to that in the positive control group. The amount of phenolic red excretion decreased in the middle and high dose groups of compound auricularia powder,and there was no difference between the groups. The effect was slightly lower than that in the positive control group. Auricularia composite powder has the effect of relieving cough and removing phlegm.
To investigate the effects of compound auricularia powder on relieving cough and expectorant. ICR mice were randomly divided into five groups: blank control group( isovolumic saline),positive control group( Chuanbei loquat dew),low( 0. 05 g/m L),medium( 0. 1 g/m L) and high( 0. 3 g/m L) dosage group. Ten rats in each group were given intragastric administration of 25 m L/kg. The equivalent dose of Chuanbei loquat ointment was 5. 85 m L/kg,the original solution was absorbed 5. 85 m L,diluted to 50 m L with normal saline,and then gavaged for 30 days,twice a day. After the last gavage,the cough induced by ammonia water and the phenol red excretion were measured. The cough latency and the number of coughs within 3 minutes were observed after 30 days of oral administration of ammonia water. The phenol red excretion test mice were given orally for 30 days and intraperitoneally injected with 5% phenol red solution 1 hour after the last oral administration to observe the amount of phenol red excretion in trachea of mice.Compared with the normal control group,the incubation period of cough induced by concentrated ammonia could be prolonged in both the positive control group and the composite auricle powder group( P < 0. 01); The cough frequency of mice induced by concentrated ammonia could be reduced( P < 0. 01); The amount of phenol red excretion from trachea of mice was increased( P < 0. 01). Compared with the positive control group,the incubation period of cough induced in the high dose group of compound auricularia powder was prolonged,the number of coughs within 3 min was reduced,and the effect of cough relief was similar to that in the positive control group. The amount of phenolic red excretion decreased in the middle and high dose groups of compound auricularia powder,and there was no difference between the groups. The effect was slightly lower than that in the positive control group. Auricularia composite powder has the effect of relieving cough and removing phlegm.
引文
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[14]杨文建,孙勇,袁彪,等.食用菌超细粉免疫调节和抗氧化功能研究[J].菌物学报,2015,34(02):301-310.
[15]刘军,王昕.黑木耳多糖的止咳化痰药理作用研究[J].实用药物与临床,2015,18(02):186-188.
[16]孔祥辉,马银鹏,杨国力,等.木耳提取物和余渣的止咳化痰功效[J].食用菌学报,2017,23(03):42-45.
[17]朱磊,王振宇.黑木耳多糖对小鼠抗疲劳作用的研究[J].营养学报,2008,30(04):430-432.
[18]黄兹英,黎其万,王继良,等.黑木耳对铅中毒大鼠排铅作用的研究[J].现代食品科技,2015,31(07):25-31.
[19]卞春,王振宇,John Shi.黑木耳多糖生物功能的研究进展[J].食品工业科技,2015,36(16):390-395.
[2]郑婧,张贵君,孙湛,等.木耳超微粉降血脂作用研究[J].西部中医药,2017,(04):20-22.
[3]刘荣,赵影,栾淑莹,等.黑木耳多糖降血脂作用的小鼠实验分析[J].食品研究与开发,2017,38(23):177-181.
[4]王辰龙,张子奇,王曼,等.黑木耳多糖的提取分离及体外抗凝血作用研究[J].食品工业科技,2013,34(09):238-241.
[5]李德海,史锦硕,周聪,等.黑木耳多糖的制备及其抗凝血功能的研究[J].安徽农业科学,2015,43(02):283-285.
[6]胡俊飞,张华,曲航,等.硫酸化黑木耳多糖的辐射防护作用研究[J].食品研究与开发,2017,38(05):6-10.
[7]张莲姬.黑木耳黑色素抗氧化作用的研究[J].食品研究与开发,2013,34(05):111-114.
[8]王明川,于涛.不同黑木耳品种抗氧化物质含量研究[J].现代农业科技,2017,(09):81-82.
[9]尹红力,赵鑫,佟丽丽,等.黑木耳多糖体外和体内降血糖功能[J].食品科学,2015,36(21):221-226.
[10]蔡润,朱雪松,屈洪艳,等.黑木耳多糖的提取及其对脑胶质瘤的抑制作用[J].华中科技大学学报(医学版),2013,42(05):578-581.
[11]宋广磊,王婧波,杜庆宝,等.黑木耳多糖改变S-(180)肉瘤细胞膜特性研究[J].中国药学杂志,2012,(04):255-261.
[12]马琪,马小娟,姚雪萍,等.黑木耳提取物对脓毒血症大鼠全身炎症反应的影响[J].新疆医科大学学报,2013,(09):1253-1257.
[13]甘霓,徐海林,吴小勇,等.黑木耳多糖AAP-10对免疫抑制小鼠的免疫调节作用[J].食品科学,2018,39(19):196-200.
[14]杨文建,孙勇,袁彪,等.食用菌超细粉免疫调节和抗氧化功能研究[J].菌物学报,2015,34(02):301-310.
[15]刘军,王昕.黑木耳多糖的止咳化痰药理作用研究[J].实用药物与临床,2015,18(02):186-188.
[16]孔祥辉,马银鹏,杨国力,等.木耳提取物和余渣的止咳化痰功效[J].食用菌学报,2017,23(03):42-45.
[17]朱磊,王振宇.黑木耳多糖对小鼠抗疲劳作用的研究[J].营养学报,2008,30(04):430-432.
[18]黄兹英,黎其万,王继良,等.黑木耳对铅中毒大鼠排铅作用的研究[J].现代食品科技,2015,31(07):25-31.
[19]卞春,王振宇,John Shi.黑木耳多糖生物功能的研究进展[J].食品工业科技,2015,36(16):390-395.