胡杨分子生物学研究进展
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摘要
胡杨具有抗盐碱、抗旱等特性,是极端干旱沙漠地区唯一的乔木树种,对于维持荒漠地区生态平衡具有重要作用。胡杨对多种逆境的高抗性和良好适应性成为近年来研究的热点。关于胡杨分子生物学方面的研究,相对于其他模式植物研究开展较晚。文中综述了近年来国内外胡杨分子生物学研究的阶段性进展,包括分子标记开发、遗传多样性检测、基因组和转录组的研究以及重要的抗逆相关功能基因的分析等;讨论了目前胡杨分子水平研究存在的不足,并对胡杨分子生物学研究的发展前景作出展望,旨在为胡杨的保护和育种提供更多的科学参考。
Populus euphratica is the only tree species that can survive in the extremely dry desert areas with its high resistance to alkali and drought,which has an important function in maintaining the eco-balance in the desertified areas. Its high resistance to multiple stresses and good adaptability makes it a research focus in recent years. The paper reviewed the phased research progress in its molecular biology at home and abroad,including molecular markers development,genetic diversity testing,genome and transcriptome analysis,characterization of key functional genes related to stress resistance. Finally we discussed the problems existing in current research and prospected the future development direction of molecular biology research. This study was aimed at providing more scientific references for the protection and breeding of P.euphratica.
Populus euphratica is the only tree species that can survive in the extremely dry desert areas with its high resistance to alkali and drought,which has an important function in maintaining the eco-balance in the desertified areas. Its high resistance to multiple stresses and good adaptability makes it a research focus in recent years. The paper reviewed the phased research progress in its molecular biology at home and abroad,including molecular markers development,genetic diversity testing,genome and transcriptome analysis,characterization of key functional genes related to stress resistance. Finally we discussed the problems existing in current research and prospected the future development direction of molecular biology research. This study was aimed at providing more scientific references for the protection and breeding of P.euphratica.
引文
[1]FAY M F,LLEDM D,KORNBLUM M M,et al.From the waters of Babylon?:Populus euphratica in Spain is clonal and probably introduced[J].Biodiversity&Conservation,1999,8(6):769-778.
[2]BRUELHEIDE H,MANEGOLD M,JANDT U.The genetical structure of Populus euphratica and Alhagi sparsifolia stands in the Taklimakan desert[C].Workshop on Sustainable Management of the Shelterbelt Vegetation of River Oases in the Taklimakan Desert,Urumqi,2004:153-160.
[3]SAITO Y,SHIRAISHI S,TANIMOTO T,et al.Genetic diversity of Populus euphratica populations in northwestern China determined by RAPD DNA analysis.[J].New Forests,2002,23(2):97-103.
[4]生光,阿不都外力·阿不都热依木,马相汝,等.RAPD标记研究新疆胡杨五个地理种群的遗传分化[J].生物技术,2008,18(5):18-21.
[5]WANG C H,LIU L X,LIU Y G.Development and characterization of20 polymorphic microsatellite markers from RAPD product in Populus euphratica[J].Conservation Genetics Resources,2015,7(3):669-671.
[6]EUSEMANN P,FEHRENZ S,SCHNITTLER M.Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica[J].Journal of Forestry Research,2009,20(3):195-198.
[7]XU F,FENG S S,WU R L,et al.Two highly validated SSRmultiplexes(8-plex)for Euphrates’poplar,Populus euphratica(Salicaceae)[J].Molecular Ecology Resources,2013,13(1):144-153.
[8]徐放,赵舒,邬荣领,等.一种优化的胡杨高效多重(12重)SSR体系[J].植物分类与资源学报,2014,36(3):365-374.
[9]PETZOLD A,PFEIFFER T,JANSEN F,et al.Sex ratios and clonal growth in dioecious Populus euphratica Oliv.,Xinjiang,Western China[J].Trees,2013,27(3):729-744.
[10]王娟.胡杨与灰杨的物种分化研究[D].兰州:兰州大学,2013.
[11]李树春,夏辉,严冬,等.不同派别杨树无性系SSR遗传距离及聚类分析[J].植物研究,2015,35(1):68-76.
[12]马涛.胡杨抗盐的基因组遗传基础研究[D].兰州:兰州大学,2014.
[13]严东辉.胡杨干旱响应转录组及NF-YB基因表达谱[D].北京:北京林业大学,2013.
[14]覃玉蓉.胡杨microRNAs的克隆及其功能研究[D].北京:北京林业大学,2011.
[15]李博生.MicorRNA调节胡杨抗逆性状的分子机制研究[D].北京:北京林业大学,2013.
[16]周韬.盐胁迫下胡杨的生理响应及miRNA表达动态变化[D].北京:北京林业大学,2014.
[17]SI J N,ZHOU T,BO W H,et al.Genome-wide analysis of saltresponsive and novel microRNAs in Populus euphratica by deep sequencing[J].BMC Genetics,2014,15(Suppl 1):S6.DOI:10.1186/1471-2156-15-S1-S6.
[18]周婧.利用BIBAC文库建立胡杨转化基因组学平台及鉴定胡杨耐盐基因(簇)的研究[D].北京:中国林业科学研究院,2013.
[19]GUO Y H,SONG X Q,ZHAO S T,et al.A transient gene expression system in Populus euphratica Oliv.protoplasts prepared from suspension cultured cells[J].Acta Physiologiae Plantarum,2015,37(8):1-8.
[20]王东洋,黄月娇,李欢,等.利用胡杨F1代愈伤组织定位耐盐基因[J/OL].分子植物育种,2018.(2018-03-05)[2018-09-18].http://kns.cnki.net/kcms/detail/46.1068.S.20180305.1237.006.html.
[21]罗文春.多重转录组比较研究胡杨和灰杨耐盐机制[D].兰州:兰州大学,2017.
[22]ZHANG Y N,WANG Y,SA G,et al.Populus euphraticaJ3mediates root K+/Na+homeostasis by activating plasma membrane H+-ATPase in transgenic Arabidopsis under Na Cl salinity[J].Plant Cell Tissue and Organ Culture,2017,131(1):75-88.
[23]ZHANG H C,LYU F L,HAN X,et al.The calcium sensor Pe CBL1,interacting with PeCIPK24/25 and PeCIPK26,regulates Na+/K+homeostasis in Populus euphratica[J].Plant Cell Reports,2013,32(5):611-621.
[24]LYU F L,ZHANG H C,XIA X L,et al.Expression profiling and functional characterization of a CBL-interacting protein kinase gene from Populus euphratica[J].Plant Cell Reports,2014,33(5):807-818.
[25]TANG S,LIANG H Y,YAN D H,et al.Populus euphratica:the transcriptomic response to drought stress[J].Plant Molecular Biology,2013,83(6):539-557.
[26]CHEN J H,TIAN Q Q,PANG T,et al.Deep-sequencing transcriptome analysis of low temperature perception in a desert tree,Populus euphratica[J].BMC Genomics,2014,15(1):326.
[27]邓澍荣.胡杨Apyrase2调控囊泡运输和胞外ATP增强植物低温耐受机制研究[D].北京:北京林业大学.2016.
[28]TIAN Q Q,CHEN J H,WANG D,et al.Overexpression of a Populus euphratica CBF4 gene in poplar confers tolerance to multiple stresses[J].Plant Cell Tissue and Organ Culture,2017,128(2):391-407.
[29]WANG J Y,WANG J P,YANG H F.Identification and functional characterization of the NAC gene promoter from Populus euphratica[J].Planta,2016,244(2):417-427.
[30]YAN D H,XIA X L,YIN W L.NF-YB family genes identified in a poplar genome-wide analysis and expressed in Populus euphratica are responsive to drought stress[J].Plant Molecular Biology Reporert,2013,31(2):363-370.
[31]JIA H X,LI J B,ZHANG J,et al.Genome-wide survey and expression analysis of the stress-associated protein gene family in desert poplar,Populus euphratica[J].Tree Genetics&Genomes,2016,12(4):1-16.
[32]MENG K B,WU Y X.Molecular evolution of glutathione peroxidase(Gpx)family genes in desert poplar(Populus euphratica Oliv.)[J].Tree Genetics&Genomes,2017,13(2):42-47.
[33]TIAN X H,XUAN L J,LIU B Y,et al.Effects of heterologous expression of Populus euphratica brassinosteroids biosynthetic enzyme genes CPD(PeCPD)and DWF4(PeDWF4)on tissue dedifferentiation and growth of Arabidopsis thaliana seedlings[J].Plant Cell Tissue and Organ Culture,2017(2):1-11.
[34]陈永富,王阳,冯倩,等.异源表达胡杨PeCPD基因提高烟草对盐、高氮和干旱胁迫的抗性[J].应用与环境生物学报,2017,23(2):225-231.
[35]岳宁,郑彩霞.胡杨异形叶蛋白质表达的电泳分析[J].安徽农业科学,2010,38(32):58-61.
[36]王玉丽,焦培培,顾亚亚,等.胡杨叶形变化与POD同工酶的关系研究[J].干旱区资源与环境,2013,4(27):181-186.
[37]张肖.胡杨异形叶表达谱分析[D].新疆阿拉尔:塔里木大学,2016.
[38]秦少伟,李才林,郭忠忠,等.参与胡杨异形叶发生的发育相关基因表达模式及作用[J].基因组学与应用生物学,2017,36(9):56-58.
[39]秦少伟,赵利峰,张娜,等.rot3、rot4、an和spk1在胡杨异形叶发生中的表达模式和作用[J].分子植物育种,2016,10(14):2630-2636.
[40]陆梦婷,周媛媛,吕建兵,等.参与胡杨异形叶发生的长非编码RNA筛选、鉴定和功能分析[J].分子植物育种,2016,11(14):3041-3046.
[41]ZHAO L F,QIN S W.Expression profiles of miRNAs in the genesis of Populus euphratica Oliv.heteromorphic leaves[J].Plant Growth Regulation,2016,81(2):1-12.
[2]BRUELHEIDE H,MANEGOLD M,JANDT U.The genetical structure of Populus euphratica and Alhagi sparsifolia stands in the Taklimakan desert[C].Workshop on Sustainable Management of the Shelterbelt Vegetation of River Oases in the Taklimakan Desert,Urumqi,2004:153-160.
[3]SAITO Y,SHIRAISHI S,TANIMOTO T,et al.Genetic diversity of Populus euphratica populations in northwestern China determined by RAPD DNA analysis.[J].New Forests,2002,23(2):97-103.
[4]生光,阿不都外力·阿不都热依木,马相汝,等.RAPD标记研究新疆胡杨五个地理种群的遗传分化[J].生物技术,2008,18(5):18-21.
[5]WANG C H,LIU L X,LIU Y G.Development and characterization of20 polymorphic microsatellite markers from RAPD product in Populus euphratica[J].Conservation Genetics Resources,2015,7(3):669-671.
[6]EUSEMANN P,FEHRENZ S,SCHNITTLER M.Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica[J].Journal of Forestry Research,2009,20(3):195-198.
[7]XU F,FENG S S,WU R L,et al.Two highly validated SSRmultiplexes(8-plex)for Euphrates’poplar,Populus euphratica(Salicaceae)[J].Molecular Ecology Resources,2013,13(1):144-153.
[8]徐放,赵舒,邬荣领,等.一种优化的胡杨高效多重(12重)SSR体系[J].植物分类与资源学报,2014,36(3):365-374.
[9]PETZOLD A,PFEIFFER T,JANSEN F,et al.Sex ratios and clonal growth in dioecious Populus euphratica Oliv.,Xinjiang,Western China[J].Trees,2013,27(3):729-744.
[10]王娟.胡杨与灰杨的物种分化研究[D].兰州:兰州大学,2013.
[11]李树春,夏辉,严冬,等.不同派别杨树无性系SSR遗传距离及聚类分析[J].植物研究,2015,35(1):68-76.
[12]马涛.胡杨抗盐的基因组遗传基础研究[D].兰州:兰州大学,2014.
[13]严东辉.胡杨干旱响应转录组及NF-YB基因表达谱[D].北京:北京林业大学,2013.
[14]覃玉蓉.胡杨microRNAs的克隆及其功能研究[D].北京:北京林业大学,2011.
[15]李博生.MicorRNA调节胡杨抗逆性状的分子机制研究[D].北京:北京林业大学,2013.
[16]周韬.盐胁迫下胡杨的生理响应及miRNA表达动态变化[D].北京:北京林业大学,2014.
[17]SI J N,ZHOU T,BO W H,et al.Genome-wide analysis of saltresponsive and novel microRNAs in Populus euphratica by deep sequencing[J].BMC Genetics,2014,15(Suppl 1):S6.DOI:10.1186/1471-2156-15-S1-S6.
[18]周婧.利用BIBAC文库建立胡杨转化基因组学平台及鉴定胡杨耐盐基因(簇)的研究[D].北京:中国林业科学研究院,2013.
[19]GUO Y H,SONG X Q,ZHAO S T,et al.A transient gene expression system in Populus euphratica Oliv.protoplasts prepared from suspension cultured cells[J].Acta Physiologiae Plantarum,2015,37(8):1-8.
[20]王东洋,黄月娇,李欢,等.利用胡杨F1代愈伤组织定位耐盐基因[J/OL].分子植物育种,2018.(2018-03-05)[2018-09-18].http://kns.cnki.net/kcms/detail/46.1068.S.20180305.1237.006.html.
[21]罗文春.多重转录组比较研究胡杨和灰杨耐盐机制[D].兰州:兰州大学,2017.
[22]ZHANG Y N,WANG Y,SA G,et al.Populus euphraticaJ3mediates root K+/Na+homeostasis by activating plasma membrane H+-ATPase in transgenic Arabidopsis under Na Cl salinity[J].Plant Cell Tissue and Organ Culture,2017,131(1):75-88.
[23]ZHANG H C,LYU F L,HAN X,et al.The calcium sensor Pe CBL1,interacting with PeCIPK24/25 and PeCIPK26,regulates Na+/K+homeostasis in Populus euphratica[J].Plant Cell Reports,2013,32(5):611-621.
[24]LYU F L,ZHANG H C,XIA X L,et al.Expression profiling and functional characterization of a CBL-interacting protein kinase gene from Populus euphratica[J].Plant Cell Reports,2014,33(5):807-818.
[25]TANG S,LIANG H Y,YAN D H,et al.Populus euphratica:the transcriptomic response to drought stress[J].Plant Molecular Biology,2013,83(6):539-557.
[26]CHEN J H,TIAN Q Q,PANG T,et al.Deep-sequencing transcriptome analysis of low temperature perception in a desert tree,Populus euphratica[J].BMC Genomics,2014,15(1):326.
[27]邓澍荣.胡杨Apyrase2调控囊泡运输和胞外ATP增强植物低温耐受机制研究[D].北京:北京林业大学.2016.
[28]TIAN Q Q,CHEN J H,WANG D,et al.Overexpression of a Populus euphratica CBF4 gene in poplar confers tolerance to multiple stresses[J].Plant Cell Tissue and Organ Culture,2017,128(2):391-407.
[29]WANG J Y,WANG J P,YANG H F.Identification and functional characterization of the NAC gene promoter from Populus euphratica[J].Planta,2016,244(2):417-427.
[30]YAN D H,XIA X L,YIN W L.NF-YB family genes identified in a poplar genome-wide analysis and expressed in Populus euphratica are responsive to drought stress[J].Plant Molecular Biology Reporert,2013,31(2):363-370.
[31]JIA H X,LI J B,ZHANG J,et al.Genome-wide survey and expression analysis of the stress-associated protein gene family in desert poplar,Populus euphratica[J].Tree Genetics&Genomes,2016,12(4):1-16.
[32]MENG K B,WU Y X.Molecular evolution of glutathione peroxidase(Gpx)family genes in desert poplar(Populus euphratica Oliv.)[J].Tree Genetics&Genomes,2017,13(2):42-47.
[33]TIAN X H,XUAN L J,LIU B Y,et al.Effects of heterologous expression of Populus euphratica brassinosteroids biosynthetic enzyme genes CPD(PeCPD)and DWF4(PeDWF4)on tissue dedifferentiation and growth of Arabidopsis thaliana seedlings[J].Plant Cell Tissue and Organ Culture,2017(2):1-11.
[34]陈永富,王阳,冯倩,等.异源表达胡杨PeCPD基因提高烟草对盐、高氮和干旱胁迫的抗性[J].应用与环境生物学报,2017,23(2):225-231.
[35]岳宁,郑彩霞.胡杨异形叶蛋白质表达的电泳分析[J].安徽农业科学,2010,38(32):58-61.
[36]王玉丽,焦培培,顾亚亚,等.胡杨叶形变化与POD同工酶的关系研究[J].干旱区资源与环境,2013,4(27):181-186.
[37]张肖.胡杨异形叶表达谱分析[D].新疆阿拉尔:塔里木大学,2016.
[38]秦少伟,李才林,郭忠忠,等.参与胡杨异形叶发生的发育相关基因表达模式及作用[J].基因组学与应用生物学,2017,36(9):56-58.
[39]秦少伟,赵利峰,张娜,等.rot3、rot4、an和spk1在胡杨异形叶发生中的表达模式和作用[J].分子植物育种,2016,10(14):2630-2636.
[40]陆梦婷,周媛媛,吕建兵,等.参与胡杨异形叶发生的长非编码RNA筛选、鉴定和功能分析[J].分子植物育种,2016,11(14):3041-3046.
[41]ZHAO L F,QIN S W.Expression profiles of miRNAs in the genesis of Populus euphratica Oliv.heteromorphic leaves[J].Plant Growth Regulation,2016,81(2):1-12.