抗菌肽CJH对常见畜禽病原菌抑菌活性及与抗生素协同杀菌效应研究
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摘要
抗菌肽CJH是从金环蛇毒液中提取出的一种抗菌活性极强的免疫活性多肽物质。试验测定了抗菌肽CJH对不同革兰氏阴性菌和阳性菌的抑菌活性及杀菌速率,并与常用抗生素的测定结果进行了对比;通过微量肉汤稀释棋盘实验法测定了抗菌肽CJH联用不同抗生素的杀菌效果。结果表明:相比几种常用抗生素,抗菌肽CJH具有广谱的抗菌活性,且杀菌能力较强;抗菌肽CJH与阿莫西林、土霉素联用有协同效应。试验为抗菌肽CJH的实际应用及为了探明其减少甚至替代抗生素成为新型饲料添加剂的潜力提供参考。
Antibacterial peptide CJH is an immunologically active peptide substance with strong antibacterial activity extracted from the snake venom. In this experiment, the antibacterial activity and sterilization rate of the antibacterial peptide CJH against different Gram-negative bacteria and positive bacteria were determined, and compared with the results of commonly used antibiotics; the antibacterial peptide CJH was determined by micro-broth dilution checkerboard method. Combine the bactericidal effect of different antibiotics. The results showed that compared with several commonly used antibiotics, the antibacterial peptide CJH has broad-spectrum antibacterial activity and strong bactericidal ability; the antibacterial peptide CJH has synergistic effect with amoxicillin and oxytetracycline. This experiment provides a theoretical basis for the practical application of the antibacterial peptide CJH, and finds its potential to reduce or even replace antibiotics into new feed additives.
Antibacterial peptide CJH is an immunologically active peptide substance with strong antibacterial activity extracted from the snake venom. In this experiment, the antibacterial activity and sterilization rate of the antibacterial peptide CJH against different Gram-negative bacteria and positive bacteria were determined, and compared with the results of commonly used antibiotics; the antibacterial peptide CJH was determined by micro-broth dilution checkerboard method. Combine the bactericidal effect of different antibiotics. The results showed that compared with several commonly used antibiotics, the antibacterial peptide CJH has broad-spectrum antibacterial activity and strong bactericidal ability; the antibacterial peptide CJH has synergistic effect with amoxicillin and oxytetracycline. This experiment provides a theoretical basis for the practical application of the antibacterial peptide CJH, and finds its potential to reduce or even replace antibiotics into new feed additives.
引文
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[14]刘倚帆,徐良,朱海燕,等.抗菌肽与抗生素对革兰氏阴性菌和革兰氏阳性菌的体外协同抗菌效果研究[J].动物营养学报,2010,22(5):1457-1463.
[2]高琼,黄海辉.艰难梭菌耐药性及耐药机制研究进展[J].遗传,2015(5):458-464.
[3]庞峰,李艳华,贾秀芹,等.耐碳青霉烯类肠杆菌科细菌的耐药性分析及基因型研究[J].临床检验杂志,2013(4):307-309.
[4]杜凤霞,贾靖,王艳鸿,等.医院多重耐药菌的耐药机制及防控措施[J].中国卫生产业,2014(14):195-196.
[5]刘倚帆.动物源抗菌肽的分子改良及其对猪肠道上皮屏障功能的保护作用研究[D].杭州:浙江大学,2012.
[6]梁英.抗菌肽的来源及应用[J].动物营养学报,2014,26(1):7-16.
[7]刘贺,徐学明.猪防御素在饲料工业中的应用和展望[J].粮食与饲料工业,2004(3):35-36.
[8]张海文,徐欣欣,洪枫,等.抗菌肽CJH对LPS刺激的仔猪生长性能及肉品质的影响[J].黑龙江畜牧兽医,2017(22):154-156.
[9]Yi H,YU C,Zhang H,et al.Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-kappaB signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets[J].International Immunopharmacology,2015,28:61-69.
[10]Han F,Lu Z,Liu Y,et al.Cathelicidin-BF ameliorates lipopolysaccharide-induced intestinal epithelial barrier disruption in rat[J].Life Sciences,2016,152:199-209.
[11]Zhang H,Xia X,Han F,et al.Cathelicidin-BF,a Novel Antimicrobial Peptide from Bungarus fasciatus,Attenuates Disease in a Dextran Sulfate Sodium Model of Colitis[J].Molecular Pharmaceutics,2015,12:1648-1661.
[12]Wakabayashi H,Teraguchi S,Tamura Y.Increased Staphylococcus-killing activity of an antimicrobial peptide,lactoferricin B,with minocycline and monoacylglycerol[J].Bioscience Biotechnology and Biochemistry,2002,66:2161-2167.
[13]王国栋.抗菌肽Protegrin-1与抗生素对致病性大肠杆菌的体外联合药效[J].河南农业科学,2013,42(8):124-127.
[14]刘倚帆,徐良,朱海燕,等.抗菌肽与抗生素对革兰氏阴性菌和革兰氏阳性菌的体外协同抗菌效果研究[J].动物营养学报,2010,22(5):1457-1463.