苦参碱抑制HepG2肝癌细胞的靶点——ERK信号通路
摘要
目的观察苦参碱在ERK信号通路中对肝癌细胞的抑制靶点。方法实验分为空白对照组、实验组、阳性对照组3组,空白对照组加入不含药的细胞培养基,实验组分别加入苦参碱,使其终浓度为1、2、4 mg/mL,阳性对照组加用终浓度为20μmol/L的ERK信号通路阻断剂PD98059,培养24 h后,用逆转录聚合酶链反应(RT-PCR)检测苦参碱对ERK信号通路的调控作用。结果苦参碱作用后HepG2细胞的ERK核酸表达下降,并通过下调p-ERK蛋白的表达量(P<0.05)抑制ERK信号通路。结论 ERK信号通路是苦参碱抑制肝癌细胞的靶点之一。
引文
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[ 2 ] Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin,2015,65: 5-29.
[ 3 ] 赵夏平.MIF通过 MAPK 信号通路促进乙肝肝硬化癌变的可能机制初步探讨.兰州:兰州大学,2014:1-50.
[ 4 ] 杨婉,王兆林,王盖浩,等.苦参碱通过相关信号通路抑制恶性肿瘤的研究进展.肿瘤研究与临床,2016, 28:63-68.
[ 5 ] Liu SQ, Zhang ML, Zhang HJ, et al. Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway.Life Sci,2017,180:36-41.
[ 6 ] 杨婉,王盖昊,王兆林,等.苦参碱对人肝癌HepG2细胞的作用机制研究.中西医结合肝病杂志,2016,26:99-101.
[ 7 ] Wu L, Liu S, Wei J, et al. Synthesis and biological evaluation of matrine derivatives as anti-hepatocellular cancer agents. Bioor Med Chem Lett, 2016, 26: 4267-4271.
[ 8 ] Lim H, Jang S, Lee Y, et al. Enhancement of anxiety and modulation of TH and pERK expressions in amygdala by repeated injections of corticosterone. Biomol Ther(Seoul), 2012, 20:418-424.
[ 9 ] Kohno M, Pouyssegur J. Targeting the ERK signaling pathway in cancer therapy. Ann Med,2006, 38:200-211.
[10] Saidak Z, Giacobbi AS, Louandre C, et al. Mathematical mode-lling unveils the essential role of cellular phosphatases in the inhibition of RAF-MEK-ERK signalling by sorafenib in hepato-cellular carcinoma cells.Cancer Letters, 2017,392:1-8.