针刺长强穴对自闭症模型大鼠海马区相关蛋白表达的影响
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摘要
目的观察针刺长强穴对自闭症模型大鼠学习记忆功能、海马区β-catenin和GSK-3β蛋白表达的影响。方法选健康成年Wistar雄性及雌性大鼠各10只,进行雌、雄合笼,随机分为2组,一组在怀孕12 d时给予母鼠腹腔注射丙戊酸钠,其产下的仔鼠随机分为非针刺对照组10只、非穴治疗对照组10只、针刺"长强"穴组9只。一组产下空白对照组。治疗结束后行水迷宫实验检测及免疫组化技术检测。结果针刺长强穴组及空白对照组与非针刺对照模型组相比,其平均逃避潜伏期明显缩短(P<0.05),其海马区β-catenin蛋白阳性细胞率明显下调(P<0.05),其海马区GSK-3β蛋白阳性细胞率明显上调(P<0.05)。结论针刺"长强"穴可通过调节Wnt通路上的β-catenin和GSK-3β蛋白产生影响进而提高自闭症模型大鼠学习记忆能力。
Objective To study the effet of acupuncture at Changqiang(GV1) on the learning and memory function in rat models of autism as well as on the β-catenin and GSK-3β protein expression of hippocampus region. Methods Choosing 10 healthy adult Wistar male rats and female rats, they were randomly divided into 2 groups. One group was injected with valproate after 12 days of pregnancy, and the birth of rats were randomly divided into a non-acupoint group, a model group, an electroacupuncture at Changqiang group. Another group gave birth to a blank control group. After treatment, the water maze test and immunohistochemical test were performed. Results Compared with the non-acupoint group, the average escape latency was significantly shortened(P<0.05)by a blank control group and an electroacupuncture at Changqiang group, the hippocampus β-catenin protein positive cells rate significantly lowered(P<0.05), and the hippocampus GSK-3β protein positive cells rate significantly raised(P<0.05). Conclusion The acupuncture at Changqiang(GV1) can be regulated by Wnt pathway on the β-catenin and GSK-3β protein protein and improve learning and memory ability in rat models of autism.
Objective To study the effet of acupuncture at Changqiang(GV1) on the learning and memory function in rat models of autism as well as on the β-catenin and GSK-3β protein expression of hippocampus region. Methods Choosing 10 healthy adult Wistar male rats and female rats, they were randomly divided into 2 groups. One group was injected with valproate after 12 days of pregnancy, and the birth of rats were randomly divided into a non-acupoint group, a model group, an electroacupuncture at Changqiang group. Another group gave birth to a blank control group. After treatment, the water maze test and immunohistochemical test were performed. Results Compared with the non-acupoint group, the average escape latency was significantly shortened(P<0.05)by a blank control group and an electroacupuncture at Changqiang group, the hippocampus β-catenin protein positive cells rate significantly lowered(P<0.05), and the hippocampus GSK-3β protein positive cells rate significantly raised(P<0.05). Conclusion The acupuncture at Changqiang(GV1) can be regulated by Wnt pathway on the β-catenin and GSK-3β protein protein and improve learning and memory ability in rat models of autism.
引文
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[11] M ajum der M,D utta S,Barm an R N,et a1. Association between autism and variants in the win-gless-type MMTV integration site family member 2(WNT2)gene[J]. Intemational Journal of Neuropsychopharmacology,2010,13(4):443-449.
[12]Kalkman H O.A reviewof the evidence for the canonica1 Wnt-pathway in autism spectrum disorders[J]. Molecular Autjsm 2012,3(15):81-90.
[13]陈天红,孙莉. Wnt信号通路在自闭症疾病中的作用[J].华夏医学,2016,5(3):162-166.
[2]张学君,洪霖,洪钰竺,等.电针后海穴对自闭症模型大鼠学习和记忆能力的影响[J].福建中医药大学学报,2013,23(1):13-15.
[3] Stanfield A C,Mclniosh A M,SPeneerM D,et al. Toward aneuroanatomyofautism:Asystematiereviewandmeta-analysisofstruetural magnctieresonance imaging stUdies[J].EurPsyehlatry,2008,23(4):1-11.
[4]陈明军,于剑锋,柴继侠,等. wnt通路信号蛋白β-catenin和GSK-3β在孤独症模型大鼠脑中表达的变化[J].神经解剖学杂志,2009,25(4):361-368.
[5]张应花,贾云杰,张天然,等. wnt/β-catenin信息通路对孤独症模型大鼠重复呆板样行为的影响[J].中国临床解剖学杂志,2015,33(4):430-433,443.
[6]李忠仁.实验针灸学[M].北京;中国中医药出版社,2003:326.
[7]诸葛启钏.大鼠脑立体定位图谱[M].北京:人民卫生出版社,2005:16-20.
[8]吴强,韩平,王振宇,等.针刺长强穴治疗精神发育迟滞患儿57例多中心临床研究报告[J].中华中医药杂志,2011,26(11):2668-2671.
[9]朱震亨.格致余论[M].北京:人民卫生出版社,1963:48-53.
[10]尹定子,宋海云. Wnt信号通路:调控机理和生物学意义[J].中国细胞生物学学报,2011,33(2):103-111.
[11] M ajum der M,D utta S,Barm an R N,et a1. Association between autism and variants in the win-gless-type MMTV integration site family member 2(WNT2)gene[J]. Intemational Journal of Neuropsychopharmacology,2010,13(4):443-449.
[12]Kalkman H O.A reviewof the evidence for the canonica1 Wnt-pathway in autism spectrum disorders[J]. Molecular Autjsm 2012,3(15):81-90.
[13]陈天红,孙莉. Wnt信号通路在自闭症疾病中的作用[J].华夏医学,2016,5(3):162-166.