石柱黄连主要生物碱的测定
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摘要
采用高效液相色谱法(HPLC)测定黄连(Coptis chinensis)主要生物碱。结果表明,石柱不同栽培片区黄连不同部位主要生物碱含量均存在差异,且4种生物碱的含量高低顺序均为:小檗碱>黄连碱>表小檗碱>巴马汀,可为黄连规范化种植及其副产品的综合开发利用提供理论依据和应用参考价值。
Through the determination and analysis of the main alkaloids of different parts of Coptis chinensis in different cultivation areas of Shizhu, the basis for reasonable planting and comprehensive development of Coptis chinensis was provided.Quantitative Determination of the main alkaloids from Coptis chinensis by HPLC methods. The main alkaloids of different parts of Coptis chinensis in different areas were differences of Sizhu. These four alkaloids' content were in the order of epiberberine> coptisine> palmatine> berberine. The result canbe used to provide theoretical basis and application reference value for the comprehensive development and utilization of standardized planting and its by-products of Coptis chinensis.
Through the determination and analysis of the main alkaloids of different parts of Coptis chinensis in different cultivation areas of Shizhu, the basis for reasonable planting and comprehensive development of Coptis chinensis was provided.Quantitative Determination of the main alkaloids from Coptis chinensis by HPLC methods. The main alkaloids of different parts of Coptis chinensis in different areas were differences of Sizhu. These four alkaloids' content were in the order of epiberberine> coptisine> palmatine> berberine. The result canbe used to provide theoretical basis and application reference value for the comprehensive development and utilization of standardized planting and its by-products of Coptis chinensis.
引文
[1]中国科学院中国植物志编辑委员会.(第27卷)[M].北京:科学出版社,1979.
[2]向莉,李盾.HPLC法研究黄连中表小檗碱等五种生物碱的含量[J].成都中医药大学学报,2004,27(2):46-48.
[3]国家药典委员会.中华人民共和国药典一部(2010版)[M].北京:中国药业科技出版社,2010.
[4]中国医学科学院药用植物资源开发研究所.中国药用植物栽培学[M].北京:农业出版社,1991.
[5]周丽,杨建,詹志来,等.不同产地黄连中重金属的含量测定及不同用药方式下黄连重金属的风险评估[J].药学学报,2018,53(3):432-438.
[6]陈兴福,卢德蓉,刘岁荣,等.味连生态环境与土壤理化特性研究[J].土壤农化通报,1994,9(3):1-6.
[7]宫涛,魏盼盼,张正海.黄连规范化栽培方法[J].特种经济动植物,2009,12(11):37-39.
[8]陈震,王淑芬.黄连丰产栽培技术[M].北京:中国农业出版社,2002.
[9]尹清.HPLC测定黄连中盐酸小檗碱含量[J].上海中医药杂志,2003,37(7):53-55.
[10]武小赟,李铁钢,阳勇,等.HPLC法测定黄连中主要生物碱的方法学研究[J].上海中医药杂志,2010(6):112-115.
[11]曾烨,王学奎,薛翔楠,等.湖北利川黄连不同生长龄期主要生物碱的分布及变化[J].中国实验方剂学杂志,2013,19(11):123-126.
[2]向莉,李盾.HPLC法研究黄连中表小檗碱等五种生物碱的含量[J].成都中医药大学学报,2004,27(2):46-48.
[3]国家药典委员会.中华人民共和国药典一部(2010版)[M].北京:中国药业科技出版社,2010.
[4]中国医学科学院药用植物资源开发研究所.中国药用植物栽培学[M].北京:农业出版社,1991.
[5]周丽,杨建,詹志来,等.不同产地黄连中重金属的含量测定及不同用药方式下黄连重金属的风险评估[J].药学学报,2018,53(3):432-438.
[6]陈兴福,卢德蓉,刘岁荣,等.味连生态环境与土壤理化特性研究[J].土壤农化通报,1994,9(3):1-6.
[7]宫涛,魏盼盼,张正海.黄连规范化栽培方法[J].特种经济动植物,2009,12(11):37-39.
[8]陈震,王淑芬.黄连丰产栽培技术[M].北京:中国农业出版社,2002.
[9]尹清.HPLC测定黄连中盐酸小檗碱含量[J].上海中医药杂志,2003,37(7):53-55.
[10]武小赟,李铁钢,阳勇,等.HPLC法测定黄连中主要生物碱的方法学研究[J].上海中医药杂志,2010(6):112-115.
[11]曾烨,王学奎,薛翔楠,等.湖北利川黄连不同生长龄期主要生物碱的分布及变化[J].中国实验方剂学杂志,2013,19(11):123-126.