巴西橡胶树NAC基因家族5个成员的荧光原位杂交物理定位
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摘要
NAC转录因子是在植物的境胁迫应答、生长发育以及激素调节中具有重要的功能一类转录调控因子。为了研究巴西橡胶树NAC基因家族中5个成员(HbNAC1,HbNAC2,HbNAC3,HbNAC24,HbNAC33)在染色体的具体位置,以巴西橡胶树(Hevea brasiliensis)无性系热研7-33-97品种为材料,利用荧光原位杂交技术对其进行了物理定位分析。结果表明:HbNAC1、HbNAC3、HbNAC33基因分别位于第5号、第9号和第2号染色体的短臂上,对应的信号位点距着丝粒平均百分距分别为73.09、27.42和26.53;HbNAC24、HbNAC2基因分别定位在巴西橡胶树的第6号和第3号染色体的长臂区域,其所对应着的着丝粒与信号位点之间的百分距离平均值为63.67和66.10。本研究通过物理定位确定这些基因在染色体上的具体位置和相互关系,在基因组学、分子辅助育种等研究方面有很好的应用前景。
The NAC transcription factors are important transcription regulators in environmental stress response,growth and development,and hormone regulation in plants.In order to reveal specific location of the five members of NAC gene family(HbNAC1,HbNAC2,HbNAC3,HbNAC24,HbNAC33) on chromosomes of Hevea brasiliensis,Hevea brasiliensis cultivar Reyan 7-33-97 was selected as materials,the physical localization analysis of which was carried out by fluorescence in situ hybridization(FISH).The results showed that HbNAC1,HbNAC3,and HbNAC33 genes were located on the short arm of chromosome 5,chromosome 9 and chromosome 2,respectively,and the average distance between the signal and the true silk were 73.09,27.42 and 26.53,respectively;HbNAC2 and HbNAC24 genes were located on the long arm of chromosome 3 and chromosome 6,and the average distance between the signal and the true silk were 66.10 and 63.67.The location and interrelationship of these genes on chromosomes were determined by physical position in this study,which might have a good application prospects in genomics,molecular assisted breeding and other research aspects.
The NAC transcription factors are important transcription regulators in environmental stress response,growth and development,and hormone regulation in plants.In order to reveal specific location of the five members of NAC gene family(HbNAC1,HbNAC2,HbNAC3,HbNAC24,HbNAC33) on chromosomes of Hevea brasiliensis,Hevea brasiliensis cultivar Reyan 7-33-97 was selected as materials,the physical localization analysis of which was carried out by fluorescence in situ hybridization(FISH).The results showed that HbNAC1,HbNAC3,and HbNAC33 genes were located on the short arm of chromosome 5,chromosome 9 and chromosome 2,respectively,and the average distance between the signal and the true silk were 73.09,27.42 and 26.53,respectively;HbNAC2 and HbNAC24 genes were located on the long arm of chromosome 3 and chromosome 6,and the average distance between the signal and the true silk were 66.10 and 63.67.The location and interrelationship of these genes on chromosomes were determined by physical position in this study,which might have a good application prospects in genomics,molecular assisted breeding and other research aspects.
引文
Aida M.,Ishida T.,Fukaki H.,Fujisawa H.,and Tasaka M.,1997,Genes involved in organ separation in Arabidopsis:an analysis of the cup-shaped cotyledon mutant,Plant Cell,9(6):841-857
Gao H.Q.,Wang Y.,Jin G.,Qiu H.Y.,and Zhuang N.S.,2009a,Optimization of chromosome preparations of Hevea leaves,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),30(5):565-569(高和琼,王英,金鸽,邱海燕,庄南生,2009a,橡胶树叶片染色体制片方法的优化,热带作物学报,30(5):565-569)
Gao H.Q.,Zhuang N.S.,Wang Y.,and Qiu H.Y.,2009b,Karyotype analysis of two varieties in Hevea brasiliensis,Zhiwu Kexue Xuebao(Journal of Wuhan Botanical Research),27(5):537-540(高和琼,庄南生,王英,邱海燕,2009b,橡胶树两个品种的核型分析,植物科学学报,27(5):537-540)
Guan J.Y.,Wang Y.,Gao H.Q.,and Zhuang N.S.,2014,Physical location of four Hb RZF genes from rubber tree(Hevea brasiliensis),Jiyin Zuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(3):610-616(官锦燕,王英,高和琼,庄南生,2014,巴西橡胶树4个环锌指蛋白基因(Hb RZF)的物理定位,基因组学与应用生物学,33(3):610-616)
Kang G.J.,Li Y.,and Zeng R.Z.,2014a,Clone and expression of Hb NAC24 gene from Hevea brasiliensis,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),34(12):2374-2381(康桂娟,黎瑜,曾日中,2014a,巴西橡胶树Hb NAC24基因克隆和表达分析,西北植物学报,34(12):2374-2381)
Kang G.J.,Li Y.,and Zeng R.Z.,2014b,Molecular cloning and expression analysis of Hb NAC33 from Hevea brasiliensis Müll.Arg.,Jiyin Zuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(4):845-852(康桂娟,黎瑜,曾日中,2014b,巴西橡胶树Hb NAC33基因的克隆和表达分析,基因组学与应用生物学,33(4):845-852)
Kang G.J.,Li Y.,and Zeng R.Z.,2015,Clone and expression analysis on gene Hb NTL2 from Hevea brasiliensis Muell.Arg.,Hunan Nongye Daxue Xuebao(Journal of Hunan A-gricultural University(Natural Sciences)),41(6):602-609(康桂娟,黎瑜,曾日中,2015,巴西橡胶树Hb NTL2基因的克隆和表达分析,湖南农业大学学报(自然科学版),41(6):602-609)
Kang G.J.,Li Y.,and Zeng R.Z.,2016,Clone and expression analysis of Hb NAM from Hevea brasiliensis Muell.Arg.Nanjing Linye Daxue Xuebao(Journal of Nanjing Forestry University(Natural Sciences Edition)),40(1):59-64(康桂娟,黎瑜,曾日中,2016,巴西橡胶树Hb NAM基因克隆和表达分析,南京林业大学学报(自然科学版),40(1):59-64)
Kang G.J.,Li Y.,Zeng R.Z.,and Nie Z.Y.,2013,Cloning and sequence analysis of Hb NTL1 promoter from Hevea brasiliensis,Shengwu Jishu Tongbao(Biotechnology Bulletin),(1)83-88(康桂娟,黎瑜,曾日中,聂智毅,2013,巴西橡胶树Hb NTL1基因启动子的克隆与序列分析,生物技术通报(1):83-88)
Liu P.P.,Wang Y.,Gao H.Q.,Li K.M.,and Zhuang N.S.,2015Physical localization of SBE and GBSS genes from Cassava(Manihotesculenta Crantz),Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(10):2224-222(刘平平,王英,高和琼,李开绵,庄南生,2015,木薯淀粉合成相关基因SBE和GBSS的物理定位,分子植物育种,13(10):2224-2229)
Nuruzzaman M.,Manimekalai R.,Sharoni A.M.,Satoh K.,Kondoh H.,Ooka H.,and Kikuchi S.,2010,Genome-wide analysis of NAC transcription factor family in rice,Gene,465(1-2):30-44
Peng H.,Yu X.W.,Cheng H.Y.,Zhang H.,Shi Q.H.,Li J.G.,and Ma H.,2010,A Survey of functional studies of the plantspecific NAC transcription factor family,Zhiwu Xuebao(Chinese Bulletin of Botany),45(2):236-248(彭辉,于兴旺,成慧颖,张桦,石庆华,李建贵,麻浩,2010,植物NAC转录因子家族研究概况,植物学报,45(2):236-248)
Puranik S.,Sahu P.P.,Prem S.S.,and Prasad P.,2012,NAC proteins:regulation and role in stress tolerance,Trends in Plan Science,17(6):369-381
Qiu Q.,2012,Cloning and expression analysis of Hb NACl Hb NAC2,Hb NAC3,three Hevea brasiliensis transcription factors,Thesis for M.S.,Hainan University,Supervisor:Zhang Z.L.,pp.17-18(邱琼,2012,三个巴西橡胶树NAC类转录因子基因的克隆和表达分析,硕士学位论文,海南大学,导师:张治礼,pp.17-18)
She S.Y.,2011,Research progress and application of fluorescence in situ hybridization,Zhongguo Xiandai Yaowu Yingyong(Chinese Journal of Modern Drug Application),5(14):129-131(佘尚扬,2011,荧光原位杂交技术的研究进展和应用,中国现代药物应用,5(14):129-131)
Song Y.C.,and Gustafson J.P.,1995,The physical location of fourteen RFLP markers in rice(Oryza sativa L.),Theor.Appl.Genet.,90(1):113-119
Souer E.,van Houwelingen A.,Kloos D.,Mol J.,and Koes R.,1996,The no apical meristem,gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries,Cell,85(2):159-170
Su H.Y.,Zhang S.Z.,Yuan X.W.,Chen C.T.,Wang X.F.,and Hao Y.J.,2013,Genome-wide analysis and identification of stress-responsive genes of the NAM-ATAF1,2-CUC2 transcription factor family in apple,Plant Physiology and Biochemistry,71(2):11-21
Tang C.,Yang M.,Fang Y.,Luo Y.,Gao S.,Xiao X.,An Z.,Zhou B.,Zhang B.,Tan X.,Yeang H.Y.,Qin Y.,Yang J.,Lin Q.,Mei H.,Montoro P.,Long X.,Qi J.,Hua Y.,He Z.,Sun M.,Li W.,Zeng X.,Cheng H.,Liu Y.,Yang J.,Tian W.,Zhuang N.,Zeng R.,Li D.,He P.,Li Z.,Zou Z.,Li S.,Li C.,Wang J.,Wei D.,Lai C.Q.,Luo W.,Yu J.,Hu S.,and Huang H.,2016,The rubber tree genome reveals new insights into rubber production and species adaptation,Nature Plants,2(6):16073
Zhang M.,Chen Y.,Wu Y.N.,and Chen Z.B.,2014,Application of fluorescence in situ hybridization,Haerbin Shifan Daxue Ziran Kexue Xuebao(Natural Science Journal of Harbin Normal University),30(6):90-93(张淼,陈瑛,吴忆宁,陈兆波,2014,荧光原位杂交技术的应用进展,哈尔滨师范大学自然科学学报,30(6):90-93)
Gao H.Q.,Wang Y.,Jin G.,Qiu H.Y.,and Zhuang N.S.,2009a,Optimization of chromosome preparations of Hevea leaves,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),30(5):565-569(高和琼,王英,金鸽,邱海燕,庄南生,2009a,橡胶树叶片染色体制片方法的优化,热带作物学报,30(5):565-569)
Gao H.Q.,Zhuang N.S.,Wang Y.,and Qiu H.Y.,2009b,Karyotype analysis of two varieties in Hevea brasiliensis,Zhiwu Kexue Xuebao(Journal of Wuhan Botanical Research),27(5):537-540(高和琼,庄南生,王英,邱海燕,2009b,橡胶树两个品种的核型分析,植物科学学报,27(5):537-540)
Guan J.Y.,Wang Y.,Gao H.Q.,and Zhuang N.S.,2014,Physical location of four Hb RZF genes from rubber tree(Hevea brasiliensis),Jiyin Zuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(3):610-616(官锦燕,王英,高和琼,庄南生,2014,巴西橡胶树4个环锌指蛋白基因(Hb RZF)的物理定位,基因组学与应用生物学,33(3):610-616)
Kang G.J.,Li Y.,and Zeng R.Z.,2014a,Clone and expression of Hb NAC24 gene from Hevea brasiliensis,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),34(12):2374-2381(康桂娟,黎瑜,曾日中,2014a,巴西橡胶树Hb NAC24基因克隆和表达分析,西北植物学报,34(12):2374-2381)
Kang G.J.,Li Y.,and Zeng R.Z.,2014b,Molecular cloning and expression analysis of Hb NAC33 from Hevea brasiliensis Müll.Arg.,Jiyin Zuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(4):845-852(康桂娟,黎瑜,曾日中,2014b,巴西橡胶树Hb NAC33基因的克隆和表达分析,基因组学与应用生物学,33(4):845-852)
Kang G.J.,Li Y.,and Zeng R.Z.,2015,Clone and expression analysis on gene Hb NTL2 from Hevea brasiliensis Muell.Arg.,Hunan Nongye Daxue Xuebao(Journal of Hunan A-gricultural University(Natural Sciences)),41(6):602-609(康桂娟,黎瑜,曾日中,2015,巴西橡胶树Hb NTL2基因的克隆和表达分析,湖南农业大学学报(自然科学版),41(6):602-609)
Kang G.J.,Li Y.,and Zeng R.Z.,2016,Clone and expression analysis of Hb NAM from Hevea brasiliensis Muell.Arg.Nanjing Linye Daxue Xuebao(Journal of Nanjing Forestry University(Natural Sciences Edition)),40(1):59-64(康桂娟,黎瑜,曾日中,2016,巴西橡胶树Hb NAM基因克隆和表达分析,南京林业大学学报(自然科学版),40(1):59-64)
Kang G.J.,Li Y.,Zeng R.Z.,and Nie Z.Y.,2013,Cloning and sequence analysis of Hb NTL1 promoter from Hevea brasiliensis,Shengwu Jishu Tongbao(Biotechnology Bulletin),(1)83-88(康桂娟,黎瑜,曾日中,聂智毅,2013,巴西橡胶树Hb NTL1基因启动子的克隆与序列分析,生物技术通报(1):83-88)
Liu P.P.,Wang Y.,Gao H.Q.,Li K.M.,and Zhuang N.S.,2015Physical localization of SBE and GBSS genes from Cassava(Manihotesculenta Crantz),Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(10):2224-222(刘平平,王英,高和琼,李开绵,庄南生,2015,木薯淀粉合成相关基因SBE和GBSS的物理定位,分子植物育种,13(10):2224-2229)
Nuruzzaman M.,Manimekalai R.,Sharoni A.M.,Satoh K.,Kondoh H.,Ooka H.,and Kikuchi S.,2010,Genome-wide analysis of NAC transcription factor family in rice,Gene,465(1-2):30-44
Peng H.,Yu X.W.,Cheng H.Y.,Zhang H.,Shi Q.H.,Li J.G.,and Ma H.,2010,A Survey of functional studies of the plantspecific NAC transcription factor family,Zhiwu Xuebao(Chinese Bulletin of Botany),45(2):236-248(彭辉,于兴旺,成慧颖,张桦,石庆华,李建贵,麻浩,2010,植物NAC转录因子家族研究概况,植物学报,45(2):236-248)
Puranik S.,Sahu P.P.,Prem S.S.,and Prasad P.,2012,NAC proteins:regulation and role in stress tolerance,Trends in Plan Science,17(6):369-381
Qiu Q.,2012,Cloning and expression analysis of Hb NACl Hb NAC2,Hb NAC3,three Hevea brasiliensis transcription factors,Thesis for M.S.,Hainan University,Supervisor:Zhang Z.L.,pp.17-18(邱琼,2012,三个巴西橡胶树NAC类转录因子基因的克隆和表达分析,硕士学位论文,海南大学,导师:张治礼,pp.17-18)
She S.Y.,2011,Research progress and application of fluorescence in situ hybridization,Zhongguo Xiandai Yaowu Yingyong(Chinese Journal of Modern Drug Application),5(14):129-131(佘尚扬,2011,荧光原位杂交技术的研究进展和应用,中国现代药物应用,5(14):129-131)
Song Y.C.,and Gustafson J.P.,1995,The physical location of fourteen RFLP markers in rice(Oryza sativa L.),Theor.Appl.Genet.,90(1):113-119
Souer E.,van Houwelingen A.,Kloos D.,Mol J.,and Koes R.,1996,The no apical meristem,gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries,Cell,85(2):159-170
Su H.Y.,Zhang S.Z.,Yuan X.W.,Chen C.T.,Wang X.F.,and Hao Y.J.,2013,Genome-wide analysis and identification of stress-responsive genes of the NAM-ATAF1,2-CUC2 transcription factor family in apple,Plant Physiology and Biochemistry,71(2):11-21
Tang C.,Yang M.,Fang Y.,Luo Y.,Gao S.,Xiao X.,An Z.,Zhou B.,Zhang B.,Tan X.,Yeang H.Y.,Qin Y.,Yang J.,Lin Q.,Mei H.,Montoro P.,Long X.,Qi J.,Hua Y.,He Z.,Sun M.,Li W.,Zeng X.,Cheng H.,Liu Y.,Yang J.,Tian W.,Zhuang N.,Zeng R.,Li D.,He P.,Li Z.,Zou Z.,Li S.,Li C.,Wang J.,Wei D.,Lai C.Q.,Luo W.,Yu J.,Hu S.,and Huang H.,2016,The rubber tree genome reveals new insights into rubber production and species adaptation,Nature Plants,2(6):16073
Zhang M.,Chen Y.,Wu Y.N.,and Chen Z.B.,2014,Application of fluorescence in situ hybridization,Haerbin Shifan Daxue Ziran Kexue Xuebao(Natural Science Journal of Harbin Normal University),30(6):90-93(张淼,陈瑛,吴忆宁,陈兆波,2014,荧光原位杂交技术的应用进展,哈尔滨师范大学自然科学学报,30(6):90-93)