杂交构树茎段组织培养体系的建立
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摘要
以杂交构树[Broussonetia papyrifera (L.) Vent.]——中科一号为试验材料,通过对杂交构树茎段的离体培养和扩繁,探究了不同激素配比对杂交构树诱导愈伤、丛生芽、壮苗和生根的影响。试验结果表明,以具腋芽的构树茎段为外植体材料,75%乙醇溶液浸泡40 s后,再经0.3%HgCl2灭菌15 min,外植体的成活率可达44%;诱导愈伤组织的最佳培养基为MS+IBA 0.5 mg/L+6-BA 2.0 mg/L+蔗糖25 g/L+琼脂7.0 g/L,pH 5.6～5.8,愈伤诱导率达90%;诱导丛生芽的最佳培养基为MS+IBA 1.5 mg/L+6-BA2.0 mg/L+蔗糖25 g/L+琼脂7.0 g/L,pH 5.6～5.8,丛生芽增殖系数为4.2,生长势较好;壮苗的最佳培养基为MS+IBA 0.2 mg/L+6-BA 1.5 mg/L+蔗糖25 g/L+琼脂7.0 g/L,pH 5.6～5.8,平均株高4.8 cm;生根的最佳培养基为1/2 MS+IBA 0.8 mg/L+活性炭1.5 g/L+马铃薯50 g/L+蔗糖25 g/L+琼脂7.0 g/L,pH 5.6～5.8,生根率100%。最佳的炼苗移栽基质土为珍珠岩∶蛭石∶草炭=1∶1∶1的混合基质,幼苗生长得最好,平均株高为7.3 cm。
The hybrid tree[Broussonetia papyrifera(L.) Vent.]—Zhongke No. 1 was used as the experimental material, through the culture and expansion of the stem segments of the hybrid tree in vitro, the effects of different hormones on callus, shoot buds, strong seedlings and roots were studied. The results showed that the survival rate of explant material, stems of the buds with axillary buds, could reach 44%, after immersed in 75% ethanol solution for 40 s, then sterilized by 0.3% HgCl2 for 15 min.The optimal medium for inducing callus is MS+IBA 0.5 mg/L+6-BA 2.0 mg/L+sucrose 25 g/L+agar 7.0 g/L, pH 5.6～5.8, the callus induction rate reached 90%. The optimal medium for inducing cluster buds was MS+IBA 1.5 mg/L+6-BA 2.0 mg/L+sucrose 25 g/L+agar 7.0 g/L,pH 5.6～5.8, the growth coefficient of cluster buds is 4.2, and the growth potential is good. The optimal medium for strong seedlings is MS+0.2 mg/L IBA+6-BA 1.5 mg/L+sucrose 25 g/L+agar 7.0 g/L, pH 5.6～5.8, average plant height is 4.8 cm. The optimal medium for rooting is 1/2 MS+IBA 0.8 mg/L+activated carbon 1.5 g/L+potato 50 g/L+sucrose 25 g/L+agar 7.0 g/L,pH 5.6～5.8, rooting rate is 100%. The best seedling transplanting substrate soil is a mixed substrate of peat∶perlite∶vermiculite=1∶1∶1. The seedlings grow best, with an average plant height of 7.3 cm.
The hybrid tree[Broussonetia papyrifera(L.) Vent.]—Zhongke No. 1 was used as the experimental material, through the culture and expansion of the stem segments of the hybrid tree in vitro, the effects of different hormones on callus, shoot buds, strong seedlings and roots were studied. The results showed that the survival rate of explant material, stems of the buds with axillary buds, could reach 44%, after immersed in 75% ethanol solution for 40 s, then sterilized by 0.3% HgCl2 for 15 min.The optimal medium for inducing callus is MS+IBA 0.5 mg/L+6-BA 2.0 mg/L+sucrose 25 g/L+agar 7.0 g/L, pH 5.6～5.8, the callus induction rate reached 90%. The optimal medium for inducing cluster buds was MS+IBA 1.5 mg/L+6-BA 2.0 mg/L+sucrose 25 g/L+agar 7.0 g/L,pH 5.6～5.8, the growth coefficient of cluster buds is 4.2, and the growth potential is good. The optimal medium for strong seedlings is MS+0.2 mg/L IBA+6-BA 1.5 mg/L+sucrose 25 g/L+agar 7.0 g/L, pH 5.6～5.8, average plant height is 4.8 cm. The optimal medium for rooting is 1/2 MS+IBA 0.8 mg/L+activated carbon 1.5 g/L+potato 50 g/L+sucrose 25 g/L+agar 7.0 g/L,pH 5.6～5.8, rooting rate is 100%. The best seedling transplanting substrate soil is a mixed substrate of peat∶perlite∶vermiculite=1∶1∶1. The seedlings grow best, with an average plant height of 7.3 cm.
引文
[1]周璟,陈中义,李华成.野生植物构树的生物学、生态学及园林应用[J].长江大学学报(自然科学版),2015,12(15):9-12,26.
[2]庞素秋.中药楮实子的抗衰老活性成分及其品质评价[D].上海:第二军医大学,2006.
[3]戚亚伟,李勇,赵云涛,等.构树叶对营养性肥胖小鼠脂肪代谢与抗氧化机能的影响[J].动物医学进展,2014,35(4):49-53.
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[9]李毅.杂交构树体细胞胚诱导及人工种子的制备[D].大连:大连工业大学,2015.
[10]白淑云,刘秉钺,何连芳.造纸原料新材种——杂交构树[J].湖南造纸,2008(04):7-9.
[11]张朝晖.构树体细胞胚悬浮培养关键技术研究[D].大连:大连工业大学,2016.
[12]屠焰,刁其玉,张蓉,等.杂交构树叶的饲用营养价值分析[J].草业科学,2009,26(6):136-139.
[13]刘建楠.构树叶总黄酮对肝癌HepG-2细胞增殖和凋亡作用的研究[D].桂林:桂林医学院,2012.
[14]李万仓.构树叶活性成分分析及抑菌作用研究[D].武汉:华中科技大学,2008.
[15]开伟华,寇婉青,程正,等.构树皮总黄酮提取及抗菌活性研究[J].安徽中医药大学学报,2015(3):93-96.
[16]张伟.杂交构树组培快繁技术概况[J].农业科技通讯,2016(6):295-296.
[17]刘中兵.光叶楮组织培养再生体系建立研究[D].武汉:华中农业大学,2009.
[18]刘芸,郭龙妹,任淼辉,等.杂交构树组培快繁体系的建立[J].山西农业科学,2016,44(8):1073-1076.
[19]曹帮华,宋丽红,刘欣玲.光叶楮组织培养和快速繁殖技术的研究[J].山东科学,2006,19(2):29-32.
[20]万文,刘忠华,魏会琴.杂交构树茎段组培快繁体系的建立[J].福建林业科技,2010,37(1):72-76.
[2]庞素秋.中药楮实子的抗衰老活性成分及其品质评价[D].上海:第二军医大学,2006.
[3]戚亚伟,李勇,赵云涛,等.构树叶对营养性肥胖小鼠脂肪代谢与抗氧化机能的影响[J].动物医学进展,2014,35(4):49-53.
[4]郭光振,张同华.构树纤维微观结构特点及应用前景[J].中国纤检,2011(21):76-79.
[5]张秋玉,李远发,梁芳.构树资源研究利用现状及其展望[J].广西农业科学,2009(2):217-220.
[6]王克荣,芦大兰,杨森安.构树叶汁液可以防治蔬菜害虫[J].长江蔬菜,2005(12):47.
[7]瞿晓晶,彭芳芳,尹楹富,等.构树种子油的超声强化提取及其抗氧化性研究[J].天然产物研究与开发,2014(10):1685-1689.
[8]杨小建,王金锡,胡庭兴.中国构树资源的综合利用[J].四川林业科技,2007(1):39-43.
[9]李毅.杂交构树体细胞胚诱导及人工种子的制备[D].大连:大连工业大学,2015.
[10]白淑云,刘秉钺,何连芳.造纸原料新材种——杂交构树[J].湖南造纸,2008(04):7-9.
[11]张朝晖.构树体细胞胚悬浮培养关键技术研究[D].大连:大连工业大学,2016.
[12]屠焰,刁其玉,张蓉,等.杂交构树叶的饲用营养价值分析[J].草业科学,2009,26(6):136-139.
[13]刘建楠.构树叶总黄酮对肝癌HepG-2细胞增殖和凋亡作用的研究[D].桂林:桂林医学院,2012.
[14]李万仓.构树叶活性成分分析及抑菌作用研究[D].武汉:华中科技大学,2008.
[15]开伟华,寇婉青,程正,等.构树皮总黄酮提取及抗菌活性研究[J].安徽中医药大学学报,2015(3):93-96.
[16]张伟.杂交构树组培快繁技术概况[J].农业科技通讯,2016(6):295-296.
[17]刘中兵.光叶楮组织培养再生体系建立研究[D].武汉:华中农业大学,2009.
[18]刘芸,郭龙妹,任淼辉,等.杂交构树组培快繁体系的建立[J].山西农业科学,2016,44(8):1073-1076.
[19]曹帮华,宋丽红,刘欣玲.光叶楮组织培养和快速繁殖技术的研究[J].山东科学,2006,19(2):29-32.
[20]万文,刘忠华,魏会琴.杂交构树茎段组培快繁体系的建立[J].福建林业科技,2010,37(1):72-76.