有氧运动对高血脂大鼠疾病模型血脂及ICAM-1和LFA-1的影响
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摘要
目的观察有氧运动对高脂血症大鼠脂代谢及细胞间黏附分子-1(ICAM-1)和淋巴细胞功能相关抗原-1(LFA-1)表达的影响,初步探讨其在动脉硬化过程中的作用机制。方法取8周龄健康、雄性SD大鼠120只,30只饲食标准饲料为正常对照组,90只饲喂高脂肪膳食,构建高血脂大鼠疾病模型实验组,并随机分为高脂饮食组(High fat diet,HF组)、PCSK9抑制剂组(HF+SBC-115076组)、有氧运动组(HF+有氧运动组),每组30只。HF组和HF+SBC-115076组在饲养过程中不运动,正常笼内生活,HF+SBC-115076组每周注射PCSK9抑制剂SBC-115076(8 mg/kg)一次,连续8周。HF+有氧运动组进行每周6 d、共持续8周的无负重游泳,8周后处死大鼠,心尖采血测定血清TG、TC、LDL、HDL、ICAM-1和LFA-1水平。取其胸主动脉HE染色观察主动脉病理学改变。结果成功建立高脂血症大鼠疾病模型。实验组各亚组摄食量差异无统计学意义(P> 0. 05),HF组及HF+SBC-115076组大鼠体重明显高于HF+有氧运动组(P <0. 01)。与正常对照组比较,HF组大鼠血清TG、TC、LDL、ICAM-1和LFA-1水平显著升高,HDL水平显著降低(P <0. 01);与HF组相比,HF+SBC-115076组和HF+有氧运动组大鼠血清TG、TC、LDL、ICAM-1和LFA-1水平显著降低,HDL水平显著升高(P <0. 01)。HE染色观察大鼠主动脉结构显示HF+有氧运动组内膜增厚明显改善,内皮损伤较少。结论有氧运动能降低高血脂大鼠的血清TG、TC、LDL水平,升高HDL水平,可能通过降低血清ICAM-1和LFA-1的表达水平,改善高脂饮食导致的内膜增厚及内皮损伤,发挥抗动脉硬化作用。
Objective To observe aerobic exercise on blood lipid and intercellular adhesion molecule-1(ICAM-1),lymphocyte function-associated antigen-1(LFA-1) in hyperlipidemia rats. Methods120 male Sprague Dawley(SD) rats were randomized into the 4 groups(n = 30) : normal diet group(control group),high fat group(HF group),HF + SBC-115076 group [SBC-115076,8 mg/kg·w) ] and HF+ aerobic exercise group. Rats in HF group and HF + SBC-115076 group did not exercise during feeding and lived in normal cage. Rats in HF + SBC-115076 group recieved PCSK9 inhibitor SBC-115076(8 mg/kg) injection once a week for 8 weeks. HF + aerobic exercise group underwent load-free swimming training6 days a week for 8 weeks. The rats were sacrificed 8 weeks later. The serum levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein(LDL),high density lipoprotein(HDL),ICAM-1 and LFA-1 were measured by apical blood sampling. Hematoxylin-eosin(HE) staining of thoracic aorta to observe pathological changes of aorta. Results After modeling,there was no significant difference in the food intake levels of the hyperlipidemia rats(P > 0. 05). The body weight of HF rats and HF + SBC-115076 rats increased significantly than HF + aerobic exercise rats(P < 0. 01). The levels of serum lipid profile,ICAM-1,and LFA-1 were significantly different between HF rats and control rats. Compared with HF rats,serum levels of TG,TC,LDL,ICAM-1 and LFA-1 in HF + SBC-115076 group and HF + aerobic exercise group were significantly lower and HDL levels were significantly higher. HE staining showed that the intimal thickening was significantly improved in HF + aerobic exercise group with less endothelial damage. Conclusions Aerobic exercise can reduce the levels of serum TG,TC,LDL and increase HDL level in rats with hyperlipidemia. It can improve the intimal thickening and endothelial damage caused by high-fat diet by reducing the expression levels of serum ICAM-1 and LFA-1,and has anti-atherosclerosis effect.
Objective To observe aerobic exercise on blood lipid and intercellular adhesion molecule-1(ICAM-1),lymphocyte function-associated antigen-1(LFA-1) in hyperlipidemia rats. Methods120 male Sprague Dawley(SD) rats were randomized into the 4 groups(n = 30) : normal diet group(control group),high fat group(HF group),HF + SBC-115076 group [SBC-115076,8 mg/kg·w) ] and HF+ aerobic exercise group. Rats in HF group and HF + SBC-115076 group did not exercise during feeding and lived in normal cage. Rats in HF + SBC-115076 group recieved PCSK9 inhibitor SBC-115076(8 mg/kg) injection once a week for 8 weeks. HF + aerobic exercise group underwent load-free swimming training6 days a week for 8 weeks. The rats were sacrificed 8 weeks later. The serum levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein(LDL),high density lipoprotein(HDL),ICAM-1 and LFA-1 were measured by apical blood sampling. Hematoxylin-eosin(HE) staining of thoracic aorta to observe pathological changes of aorta. Results After modeling,there was no significant difference in the food intake levels of the hyperlipidemia rats(P > 0. 05). The body weight of HF rats and HF + SBC-115076 rats increased significantly than HF + aerobic exercise rats(P < 0. 01). The levels of serum lipid profile,ICAM-1,and LFA-1 were significantly different between HF rats and control rats. Compared with HF rats,serum levels of TG,TC,LDL,ICAM-1 and LFA-1 in HF + SBC-115076 group and HF + aerobic exercise group were significantly lower and HDL levels were significantly higher. HE staining showed that the intimal thickening was significantly improved in HF + aerobic exercise group with less endothelial damage. Conclusions Aerobic exercise can reduce the levels of serum TG,TC,LDL and increase HDL level in rats with hyperlipidemia. It can improve the intimal thickening and endothelial damage caused by high-fat diet by reducing the expression levels of serum ICAM-1 and LFA-1,and has anti-atherosclerosis effect.
引文
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[14]陈岱琳,吴建华,方颖.流体剪应力作用下趋化因子CXCL12诱导的白细胞整合素LFA-1的激活[J].医用生物力学,2017,32(6):548-554. DOI:10. 16156/j. 1004-7220. 2017. 06. 011.
[15]史亚丽,辛晓林,王昌留,等.有氧游泳运动对老年高血脂大鼠脂代谢影响的研究[J].北京体育大学学报,2004,27(8):1053-1054,1067. DOI:10. 3969/j. issn. 1007-3612. 2004. 08.017.
[16]李陈鹏.有氧运动改善血管内皮功能障碍的研究进展[J].当代体育科技,2017,7(18):18,20. DOI:10. 16655/j. cnki.2095-2813. 2017. 18. 018.
[17]张鑫愉,牛燕媚,傅力.运动与代谢性炎症反应———有氧运动改善机体代谢机制研究进展[J].中国运动医学杂志,2018,37(1):66-69,82. DOI:10. 3969/j. issn. 1000-6710.2018. 01. 011.
[18]吴卫东,岳静静,王国伟,等.有氧运动预防及治疗ApoE-/-小鼠动脉粥样硬化过程中脂联素的抗炎症作用[J].中国康复医学杂志,2016,31(11):1219-1224. DOI:10. 3969/j. issn.1001-1242. 2016. 11. 008.
[2] Nocon M,Hiemann T,Müller-Riemenschneider F,et al. Association of physical activity with all-cause and cardiovascular mortality:a systematic review and meta-analysis[J]. Eur J Cardiovasc Prev Rehabil,2008,15(3):239-246. DOI:10. 1097/HJR.0b013e3282f55e09.
[3]李宏伟,邹家艳,赖敏,等.有氧运动减轻动脉粥样硬化斑块形成的研究进展[J].中国动脉硬化杂志,2017,25(8):861-864.DOI:10. 3969/j. issn. 1007-3949. 2017. 08. 019.
[4] Shakil-Ur-Rehman S,Karimi H,Gillani SA. Effects of supervised structured aerobic exercise training program on high and low density lipoprotein in patients with type II diabetes mellitus[J]. Pak J Med Sci,2017,33(1):96-99. DOI:10. 12669/pjms. 331.11758.
[5]武胜奇,张琳,熊正英,等.白藜芦醇和有氧运动对高脂饮食大鼠血脂和血管内皮功能的作用[J].中国运动医学杂志,2012,31(11):994-998. DOI:10. 3969/j. issn. 1000-6710.2012. 11. 009.
[6]吴卫东,张建军,岳静静,等.有氧运动对载脂蛋白E基因缺陷小鼠主动脉C反应蛋白表达水平的影响[J].中国康复医学杂志,2014,29(8):714-717. DOI:10. 3969/j. issn. 1001-1242.2014. 08. 004.
[7]沈玄霖,刘遂心.有氧运动对代谢综合征患者的疗效观察及其机制研究[J].中国医师杂志,2012,14(9):1175-1178.DOI:10. 3760/cma. j. issn. 1008-1372. 2012. 09. 007.
[8] Chaudhary R,Garg J,Shah N,et al. PCSK9 inhibitors:A new era of lipid lowering therapy[J]. World J Cardiol,2017,9(2):76-91. DOI:10. 4330/wjc. v9. i2. 76.
[9] Blom DJ,Dent R,Castro RC,et al. PCSK9 inhibition in the management of hyperlipidemia:focus on evolocumab[J]. Vasc Health Risk Manag,2016,12:185-197. DOI:10. 2147/VHRM.S102564.
[10] Sible AM,Nawarskas JJ,Anderson JR. PCSK9 Inhibitors:An Innovative Approach to Treating Hyperlipidemia[J]. Cardiol Rev,2016,24(3):141-152. DOI:10. 1097/CRD. 0000000000000102.
[11]李勇. PCSK9抑制剂会改变临床降脂治疗的未来吗?[J].心血管病学进展,2017,38(5):483-488. DOI:10. 16806/j. cnki.issn. 1004-3934. 2017. 05. 001.
[12]钱正瑶,李广平. PSCK9在动脉粥样硬化中的作用[J].临床荟萃,2016,31(1):108-111. DOI:10. 3969/j. issn. 1004-583X.2016. 01. 028.
[13]林忠伟,王卓,李涛,等.冠心病患者单核细胞黏附分子表达和氧化低密度脂蛋白的相关性[J].实用医学杂志,2011,27(23):4207-4209. DOI:10. 3969/j. issn. 1006-5725. 2011. 23.011.
[14]陈岱琳,吴建华,方颖.流体剪应力作用下趋化因子CXCL12诱导的白细胞整合素LFA-1的激活[J].医用生物力学,2017,32(6):548-554. DOI:10. 16156/j. 1004-7220. 2017. 06. 011.
[15]史亚丽,辛晓林,王昌留,等.有氧游泳运动对老年高血脂大鼠脂代谢影响的研究[J].北京体育大学学报,2004,27(8):1053-1054,1067. DOI:10. 3969/j. issn. 1007-3612. 2004. 08.017.
[16]李陈鹏.有氧运动改善血管内皮功能障碍的研究进展[J].当代体育科技,2017,7(18):18,20. DOI:10. 16655/j. cnki.2095-2813. 2017. 18. 018.
[17]张鑫愉,牛燕媚,傅力.运动与代谢性炎症反应———有氧运动改善机体代谢机制研究进展[J].中国运动医学杂志,2018,37(1):66-69,82. DOI:10. 3969/j. issn. 1000-6710.2018. 01. 011.
[18]吴卫东,岳静静,王国伟,等.有氧运动预防及治疗ApoE-/-小鼠动脉粥样硬化过程中脂联素的抗炎症作用[J].中国康复医学杂志,2016,31(11):1219-1224. DOI:10. 3969/j. issn.1001-1242. 2016. 11. 008.