宁乡猪不同杂交组合背最长肌肌苷酸含量与相关基因表达的关联性分析
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摘要
为了探讨猪肌苷酸含量与相关基因表达的相关性,试验采用高效液相色谱法测定宁乡猪杂交组合杜×宁、巴×宁、巴×巴宁和巴宁×巴宁背最长肌肌苷酸含量,采用实时荧光定量PCR(qPCR)技术检测背最长肌ADSL、GPAT和PURH基因的表达水平。结果表明:巴×宁猪肌苷酸含量显著高于巴×巴宁猪(P<0.05);ADSL基因在巴×宁猪中的表达量极显著高于巴×巴宁猪(P<0.01),GPAT和PURH基因在巴×宁猪中的表达量均显著高于巴×巴宁猪(P<0.05);ADSL和GPAT基因表达量与肌苷酸含量均呈显著正相关(P<0.05),PURH基因表达量与肌苷酸含量呈极显著正相关(P<0.01)。说明ADSL、GPAT和PURH基因与肌苷酸沉积密切相关,可作为猪肉品质的选择依据。
To explore the correlation between inosine monophosphate( IMP) contents and related gene expressions in pigs,high performance liquid chromatography method was used in this experiment in determining IMP contents of longissimus dorsi in crossbreed pigs including Duroc×Ningxiang,Berkshire×Ningxiang,Berkshire×( Berkshire×Ningxiang),( Berkshire×Ningxiang) ×( Berkshire×Ningxiang),and real-time quantitative PCR method was employed in detecting the expression levels of ADSL,GPAT and PURH genes. The results showed that IMP content in Berkshire×Ningxiang was significantly higher than that in Berkshire×( Berkshire×Ningxiang)( P<0. 05). The expression level of ADSL gene in Berkshire×Ningxiang was very significantly higher than that in Berkshire×( Berkshire×Ningxiang)( P< 0. 01). The levels of GPAT and PURH genes in were Berkshire × Ningxiang were significantly higher than those in Berkshire ×( Berkshire × Ningxiang)( P < 0. 05). A significantly positive correlations( P<0. 05) between expression levels of ADSL and GPAT genes and IMP contents were obtained,and a very significantly positive correlation( P<0. 01) between expression level of PURH gene and IMP contents was received. These results suggested that ADSL,GPAT and PURH genes are closely related to IMP deposition,which could serve as a basis for the selection of meat quality.
To explore the correlation between inosine monophosphate( IMP) contents and related gene expressions in pigs,high performance liquid chromatography method was used in this experiment in determining IMP contents of longissimus dorsi in crossbreed pigs including Duroc×Ningxiang,Berkshire×Ningxiang,Berkshire×( Berkshire×Ningxiang),( Berkshire×Ningxiang) ×( Berkshire×Ningxiang),and real-time quantitative PCR method was employed in detecting the expression levels of ADSL,GPAT and PURH genes. The results showed that IMP content in Berkshire×Ningxiang was significantly higher than that in Berkshire×( Berkshire×Ningxiang)( P<0. 05). The expression level of ADSL gene in Berkshire×Ningxiang was very significantly higher than that in Berkshire×( Berkshire×Ningxiang)( P< 0. 01). The levels of GPAT and PURH genes in were Berkshire × Ningxiang were significantly higher than those in Berkshire ×( Berkshire × Ningxiang)( P < 0. 05). A significantly positive correlations( P<0. 05) between expression levels of ADSL and GPAT genes and IMP contents were obtained,and a very significantly positive correlation( P<0. 01) between expression level of PURH gene and IMP contents was received. These results suggested that ADSL,GPAT and PURH genes are closely related to IMP deposition,which could serve as a basis for the selection of meat quality.
引文
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[15]徐善金,虞德兵,汪峰,等.鸭腺苷琥珀酸裂解酶基因序列特征及表达与肌肉肌苷酸含量的相关性分析[J].中国农业科学,2012,45(4):774-785.
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[17]豆腾飞,汪善荣,佟荟全,等.武定鸡和大围山微型鸡肌苷酸含量及ADSL基因表达差异研究[J].中国家禽,2017,39(14):7-10.
[2]郭辽朴.荣昌乳猪与PIC乳猪不同部位肌肉理化特性及主体风味物质研究[D].重庆:西南大学,2009.
[3]SUZUKI A,HOMMA N,FUKUDA A,et al.Effects of high pressure treatment on the flavor-related component[J].Meat Sci,1994,37(3):369-379.
[4]陈继兰,文杰,王述柏,等.鸡肉肌苷酸和肌内脂肪沉积规律研究[J].畜牧兽医学报,2005,36(8):843-845.
[5]杨俊静.猪GPAT基因克隆、变异和表达规律分析[D].哈尔滨:东北农业大学,2012.
[6]李亮,陈浩林,韩雪,等.影响地方鸡肌肉肌苷酸含量相关基因研究进展[J].中国畜禽种业,2016,12(9):138-140.
[7]朱吉,杨仕柳,彭英林.不同杂交组合猪的肉质测定[J].养猪,2005(5):47-49.
[8]朱吉,杨仕柳,孙建帮,等.部分湖南地方猪种的肉质分析[J].养猪,2007(5):65-67.
[9]陶勇,刘宗华,张牧,等.不同组合猪肌肉肌苷酸含量的分析[J].黑龙江畜牧兽医,2001(10):17.
[10]刘宗华,张牧,邢军.杂交对小梅山猪肌肉营养成分的影响[J].养猪,2002(4):31-32.
[11]刘基伟,徐日福,李奎,等.猪ADSL基因克隆及其在部分组织中的mRNA定量表达[J].畜牧与兽医,2010,42(6):22-27.
[12]李庆海,范京辉,楼立峰,等.饲粮能量差异对猪ADSL基因mRNA表达的影响[J].养猪,2013(6):57-59.
[13]杨俊静,李海涛,张冬杰,等.猪GPAT基因SNPs位点分析和表达规律的研究[J].东北农业大学学报,2012,43(3):19-23.
[14]张淑静,刘力源,殷宗俊,等.不同品种猪背最长肌肌苷酸含量及相关基因表达的比较[J].食品与发酵工业,2014,40(6):187-192.
[15]徐善金,虞德兵,汪峰,等.鸭腺苷琥珀酸裂解酶基因序列特征及表达与肌肉肌苷酸含量的相关性分析[J].中国农业科学,2012,45(4):774-785.
[16]余春林.鸡肌苷酸合成酶系相关基因表达水平变化与肌苷酸含量的关联性研究[D].成都:西南民族大学,2010.
[17]豆腾飞,汪善荣,佟荟全,等.武定鸡和大围山微型鸡肌苷酸含量及ADSL基因表达差异研究[J].中国家禽,2017,39(14):7-10.