SIRT3基因多态性与猪肉质性状的关联分析
|
摘要
为了探索SIRT3基因多态性与猪肉质性状的关联性,通过引物设计、PCR扩增、测序以及酶切分型,对SIRT3基因的突变位点进行检测和分析,确定了SIRT3基因的三种基因型,并将SIRT3基因的三种基因型与湘村黑猪、大围子猪的肉质性状进行了关联分析。结果表明:在猪SIRT3基因的第五外显子上存在一处A/G突变位点,形成AA、AG和GG三种基因型。进行猪SIRT3基因多态性分析可知,GG基因型频率和等位基因频率在两猪种中占有绝对优势,且在湘村黑猪中并未检测出AA基因型突变个体;进行猪SIRT3基因突变位点遗传多样性分析可知,湘村黑猪的多态信息含量(PIC)小于0.25,为低度多态,大围子猪的PIC含量在0.25～0.50之间,为中度多态。进行基因多态性与猪肉质性状的关联分析,在大围子猪24小时滴水损失、失水率和肌内脂肪含量三个性状中,AA基因型与AG、GG基因型相比差异显著(P<0.05);而湘村黑猪仅有肌肉色值中的亮度值(L~*值)在各基因型之间存在显著性差异(P<0.05),其余性状之间并不存在差异显著性(P>0.05)。由此得出,SIRT3基因突变基因型可以提高猪肉品质,SIRT3基因可作为影响猪肉质性状的候选基因进行深入研究分析。
This study was aimed to explore the association between polymorphism of SIRT3 gene and meat quality trait, the mutation sites of SIRT3 gene were detected and analyzed by primers design, PCR amplification, sequencing and enzyme typing, and were correlated with meat quality traits of Xiangcun black pig and Daweizi pig.The results show that: There is a A/G mutation on the fifth exon of the porcine SIRT3 gene, which forms three genotypes of AA, AG and GG. Analysis of polymorphism of SIRT3 gene showed that GG genotype frequency and allele frequency were dominant in two pig breeds, and no AA genotype mutation was detected in Xiangcun black pig. Analysis of genetic diversity of SIRT3 gene mutation showed that the PIC content of Xiangcun black pig was less than 0.25, which was low polymorphism. The PIC content of Daweizi pig was between 0.25-0.50, with moderate polymorphism. Association analysis between genetic polymorphism and meat quality traits showed that the AA genotype in 24 hour drip loss and intramuscular fat content were significantly higher than that of AG and GG genotypes(P<0.05)in Daweizi pig, and the AA genotype in water loss rate was significantly lower than that in AG and GG genotypes(P<0.05).In the Xiangcun black pig, there was a significant difference in the L~* value of the muscle(P<0.05), but there was no difference between the other characters(P>0.05).It is concluded that the mutant genotype of SIRT3 gene can improve the quality of meat. SIRT3 gene can be further studied and analyzed as a candidate gene affecting meat quality traits.
This study was aimed to explore the association between polymorphism of SIRT3 gene and meat quality trait, the mutation sites of SIRT3 gene were detected and analyzed by primers design, PCR amplification, sequencing and enzyme typing, and were correlated with meat quality traits of Xiangcun black pig and Daweizi pig.The results show that: There is a A/G mutation on the fifth exon of the porcine SIRT3 gene, which forms three genotypes of AA, AG and GG. Analysis of polymorphism of SIRT3 gene showed that GG genotype frequency and allele frequency were dominant in two pig breeds, and no AA genotype mutation was detected in Xiangcun black pig. Analysis of genetic diversity of SIRT3 gene mutation showed that the PIC content of Xiangcun black pig was less than 0.25, which was low polymorphism. The PIC content of Daweizi pig was between 0.25-0.50, with moderate polymorphism. Association analysis between genetic polymorphism and meat quality traits showed that the AA genotype in 24 hour drip loss and intramuscular fat content were significantly higher than that of AG and GG genotypes(P<0.05)in Daweizi pig, and the AA genotype in water loss rate was significantly lower than that in AG and GG genotypes(P<0.05).In the Xiangcun black pig, there was a significant difference in the L~* value of the muscle(P<0.05), but there was no difference between the other characters(P>0.05).It is concluded that the mutant genotype of SIRT3 gene can improve the quality of meat. SIRT3 gene can be further studied and analyzed as a candidate gene affecting meat quality traits.
引文
[1] NORTH B J,VERDIN E.Sirtuins:Sir2-related NAD-dependent protein deacetylases[J].Genome Biol,2004,5(5):224.
[2] FU M F,LIU M R,SAUVE A A,et al.Hormonal control of androgen receptor function through SIRT1[J].Mol Cell Biol,2006,26(21):8122-8135.
[3] AVALOS J L,CELIC I,MUHAMMAD S,et al.Structure of a sir2 enzyme bound to an acetylated p53 peptide[J].Mol Cell,2002,10(3):523-535.
[4] 时小燕,杜丽敏.Sirtuin家族成员及其生物学特性[J].国际药学研究杂志,2011,38(5):349-355.
[5] ZHAO S M,XU W,JIANG W Q,et al.Regulation of cellular metabolism by protein lysine acetylation[J].Science,2010,327(5968):1000-1004.
[6] 邓冠群.秦川牛Sirt3及Sirt5基因SNPs检测及其与体尺、肉质性状的关联性分析[D].杨凌:西北农林科技大学,2014.
[7] 王候光,马苗鹏,黄魁英,等.猪去乙酰化酶SIRT3的克隆及表达[J].中国畜牧兽医,2014,41(5):53-57.
[8] 桂林生,昝林森,王洪宝,等.SIRT1和SIRT2基因多态性及合并基因型与秦川牛肉用性状的关联分析[J].畜牧兽医学报,2015,46(10):1741-1749.
[2] FU M F,LIU M R,SAUVE A A,et al.Hormonal control of androgen receptor function through SIRT1[J].Mol Cell Biol,2006,26(21):8122-8135.
[3] AVALOS J L,CELIC I,MUHAMMAD S,et al.Structure of a sir2 enzyme bound to an acetylated p53 peptide[J].Mol Cell,2002,10(3):523-535.
[4] 时小燕,杜丽敏.Sirtuin家族成员及其生物学特性[J].国际药学研究杂志,2011,38(5):349-355.
[5] ZHAO S M,XU W,JIANG W Q,et al.Regulation of cellular metabolism by protein lysine acetylation[J].Science,2010,327(5968):1000-1004.
[6] 邓冠群.秦川牛Sirt3及Sirt5基因SNPs检测及其与体尺、肉质性状的关联性分析[D].杨凌:西北农林科技大学,2014.
[7] 王候光,马苗鹏,黄魁英,等.猪去乙酰化酶SIRT3的克隆及表达[J].中国畜牧兽医,2014,41(5):53-57.
[8] 桂林生,昝林森,王洪宝,等.SIRT1和SIRT2基因多态性及合并基因型与秦川牛肉用性状的关联分析[J].畜牧兽医学报,2015,46(10):1741-1749.