影响马精液冷冻保存效率的因素分析
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摘要
马精液冷冻保存技术对提高马的繁殖效率有重要意义,多种因素会对冷冻保存精液的精子造成损害。本文综述了精清、抗冻剂、微生物、过氧化作用、渗透压、冷冻和解冻等影响马精子冷冻保存的因素,旨在为提高马精液冷冻保存技术的效率、促进我国马产业更快发展提供理论依据。
The cryopreservation technique of semen is important in improving the breeding efficiency of equine. Many factors have been reported to damage spermatozoa in cryopreservation of equine semen. The factors of seminal plasma, cryoprotectant, microorganism, peroxidation, osmotic pressure, freezing and thawing are reviewed. It will provide the theoretical basis for improving the efficiency of cryopreservation of equine semen and promoting the development of China Horse Industry.
The cryopreservation technique of semen is important in improving the breeding efficiency of equine. Many factors have been reported to damage spermatozoa in cryopreservation of equine semen. The factors of seminal plasma, cryoprotectant, microorganism, peroxidation, osmotic pressure, freezing and thawing are reviewed. It will provide the theoretical basis for improving the efficiency of cryopreservation of equine semen and promoting the development of China Horse Industry.
引文
[1]Barker C A V,Gandier J C C.Pregnancy in the mare resulted from frozen epididymal spermatozoa[J].Can J Comp Med Vet Sci,1957,21:47-50.
[2]Samper J C,Morris C A.Current methods for stallion semen cryopreservation:a survey[J].Theriogenology,1998,49:895-903.
[3]Deng L,Duan H Y,Zhang X,et al.Advances in the research and application of artificial insemination to equids in china1935-2012[J].J Equine Vet Sci,2014,34:351-359.
[4]段洪云,张翔,邓亮,等.马精液冷冻保存技术研究进展[J].中国畜牧兽医,2012,39(11):149-153.
[5]Mina C G,Davies M.Equine reproduction physiology,breeding and stud management[M].3rd Edition.Wallingford:CABI publishing,2008.
[6]AlEssawe E M,Wallgren M,Wulf M,et al.Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm[J].Theriogenology,2018,115:99-107.
[7]Moore A I,Squires E L,Graham J K.Effect of seminal plasma on the cryopreservation of equine spermatozoa[J].Theriogenology,2005,63(9):2372-2381.
[8]Sieme H,Oldenhof H,Wolkers W F.Mode of action of cryoprotectants for sperm preservation[J].Anim Reprod Sci,2016,169:1-5.
[9]毋状元,罗永明,霍飞,等.精清浓度对马精子低温及冷冻保存效果的影响[J].中国畜牧杂志,2016,52(17):24-28.
[10]Edmond A J,Brinsko S P,Love C C,et al.Effect of centrifugal fractionation protocols on quality and recovery rate of equine sperm[J].Theriogenology,2012,77:959-966.
[11]Morrell J M,johannisson A,Strutz H,et al.Colloidal centrifugation of stallion semen:changes in sperm motility,velocity,and chromatin integrity during storage[J].J Equine Vet Sci,2009,29:24-32.
[12]Gutierrez-Cepeda L,Fernandez A,Crespo F,et al.Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure[J].Anim Reprod Sci,2011,124:85-91.
[13]Bliss S B,Voge J L,Hayden S S,et al.The impact of cushioned centrifugation protocols on semen quality of stallions[J].Theriogenology,2012,77:1232-1239.
[14]Diazjimenez M,Dorado J,Ortiz I.Cryopreservation of donkey sperm using non-permeable cryoprotectants[J].Anim Reprod Sci,2018,189:103-109
[15]Wu Z,Chen J,Luo Y,et al.The Effect of eight antioxidants on cryopreserved equine sperm quality[J].J Equine Vet Sci,2016,41:52.
[16]Macedo S,Bliebernicht M,Rocha A.Effect of two different nonpermeable cryoprotectants(NPC)on equine sperm viability after vitrification[J].Anim Reprod Sci,2018,194:18-19.
[17]Vidament M,Daire J M,Yvon J M,et al.Motility and fertility of stallion semen frozen with glycerol and/or dimethyl formamide[J].Theriogenology,2002,58:1-3.
[18]Alvarenga M A,Papa F O,Landim-Alvarenga F C,et al.Amides as cryoprotectants for freezing stallion semen:Areview[J].Anim Reprod Sci,2005,89:105-113.
[19]Melo,C,Alvarenga,M,Villaverde,A.I,et al.Comparison of different cryoprotectants on fertility of equine frozen semen[J].Biol Reprod,2007,77(1):178.
[20]Santiani A,Evangelista-Vargas S,Vargas S,et al.Cryopreservation of Peruvian Paso horse spermatozoa:dimethylacetamide preserved an optimal sperm function compared to dimethyl sulfoxide,ethylene glycol and glycerol[J].Andrologia,2016,49(6):1-6.
[21]Squires E L,Keith S L,Graham J K.Evaluation of alternative cryoprotectants for preserving stallion spermatozoa[J].Theriogenology,2004,62:1056-1065.
[22]Dietz J P,Sertich P L,Boston R C,et al.Comparison of ticarcillin and piperacillin in Kenney’s semen extender[J].Theriogenology,2007,68:848-852.
[23]Varner D D.The impact of cushioned centrifugation protocols on semen quality of stallions[J].Theriogenology,2012,77:1232-1239.
[24]Martins H S,Brito M F,Sampaio I B M,et al.Effects of antioxidants lactoferrin and catalase on stallion frozen semen[J].Reprod Fert Develop,2014,27(1):126-127.
[25]Vaez A,Mardani M,Razavi S.Impact of saffron on rat sperm membrane integrity and spermatogenesis status[J].Adv Biomed Res,2014,3:146-154.
[26]John A R,Baker M A.Oxidative stress and male reproductive biology[J].Reprod Fert Develop,2004,16:581-588.
[27]Bustamante F I C,Pederzolli C D,Sgaravatti A M,et al.Skim milk-egg yolk based semen extender compensates for nonenzymatic antioxidant activity loss during equine semen cryopreservation[J].Anim Reprod,2009,6(2):392-399.
[28]Christelle B,Peter S,Jean-Piere B.Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro[J].Mol Reprod Dev,2003,66:314-323.
[29]Franco J,Chaveiro A,Gois A,et al.Effects ofα-tocopherol and ascorbic acid on equine semen quality after cryopreservation[J].J Equine Vet Sci,2013,33(10):787-793.
[30]Wu Z,Zheng X,Luo Y,et al.Cryopreservation of stallion spermatozoa using different cryoprotectants and combinations of cryoprotectants[J].Anim Reprod Sci,2015,163:75-81.
[31]Flesch F M,Gadella B M.Dynamics of the mammalian sperm plasma membrane in the process of fertilization[J].Biochim Biophys Acta,2000,1469(3):197-235.
[32]Fang Y,Blair H,Zhong R,et al.Optimizing the freezing rate for ovine semen cryopreservation:Phospholipid profiles and functions of the plasma membrane and quality and fertilization of spermatozoa[J].Small Ruminant Res,2016,139:46-51.
[33]Papaa F O,Felício G B,Melo C M,et al.Replacing egg yolk with soybean lecithin in the cryopreservation of stallion semen[J].Anim Reprod Sci,2011,129:73-77.
[34]Parks J E,LynchD V.Lipid composition and thermotropic phase behavior of boar,bull,stallion,androoster sperm membranes[J].Cryobiology,1992,29:255-266.
[35]Daigneault B W,Graham J K,Bruemmer J E,et al.Cryopreservation of cooled semen and evaluation of sperm holding media for potential use in equine-assisted reproduction procedures[J].J Equine Vet Sci,2002,32(9):569-574.
[36]Linfor J,Pommer AC,Meyers S A.Osmotic stress induces tyrosine phosphorylation of equine sperm[J].Theriogenology,2002,58:355-358.
[37]Ball B A.Oxidative stress,osmotic stress and apoptosis:Impacts on sperm function and preservation in the horse[J].Anim Reprod Sci,2008,107:257-267.
[38]Sieme H,Harrison R A P,Petrunkina A M.Cryobiological determinants of frozen semen quality,with special reference to stallion[J].Anim Reprod Sci,2008,107:276-292.
[39]Muldrew K,McGann L E.Mechanisms of intracellular ice formation[J].Biophys J,1990,57(3):525-532.
[40]Freitas-Dell’Aqua C,Monteiro G A,Júnior J A,et al.The Effects of refrigeration temperature and storage time on apoptotic markers in equine semen[J].J Equine Vet Sci,2013,33:27-30.
[2]Samper J C,Morris C A.Current methods for stallion semen cryopreservation:a survey[J].Theriogenology,1998,49:895-903.
[3]Deng L,Duan H Y,Zhang X,et al.Advances in the research and application of artificial insemination to equids in china1935-2012[J].J Equine Vet Sci,2014,34:351-359.
[4]段洪云,张翔,邓亮,等.马精液冷冻保存技术研究进展[J].中国畜牧兽医,2012,39(11):149-153.
[5]Mina C G,Davies M.Equine reproduction physiology,breeding and stud management[M].3rd Edition.Wallingford:CABI publishing,2008.
[6]AlEssawe E M,Wallgren M,Wulf M,et al.Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm[J].Theriogenology,2018,115:99-107.
[7]Moore A I,Squires E L,Graham J K.Effect of seminal plasma on the cryopreservation of equine spermatozoa[J].Theriogenology,2005,63(9):2372-2381.
[8]Sieme H,Oldenhof H,Wolkers W F.Mode of action of cryoprotectants for sperm preservation[J].Anim Reprod Sci,2016,169:1-5.
[9]毋状元,罗永明,霍飞,等.精清浓度对马精子低温及冷冻保存效果的影响[J].中国畜牧杂志,2016,52(17):24-28.
[10]Edmond A J,Brinsko S P,Love C C,et al.Effect of centrifugal fractionation protocols on quality and recovery rate of equine sperm[J].Theriogenology,2012,77:959-966.
[11]Morrell J M,johannisson A,Strutz H,et al.Colloidal centrifugation of stallion semen:changes in sperm motility,velocity,and chromatin integrity during storage[J].J Equine Vet Sci,2009,29:24-32.
[12]Gutierrez-Cepeda L,Fernandez A,Crespo F,et al.Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure[J].Anim Reprod Sci,2011,124:85-91.
[13]Bliss S B,Voge J L,Hayden S S,et al.The impact of cushioned centrifugation protocols on semen quality of stallions[J].Theriogenology,2012,77:1232-1239.
[14]Diazjimenez M,Dorado J,Ortiz I.Cryopreservation of donkey sperm using non-permeable cryoprotectants[J].Anim Reprod Sci,2018,189:103-109
[15]Wu Z,Chen J,Luo Y,et al.The Effect of eight antioxidants on cryopreserved equine sperm quality[J].J Equine Vet Sci,2016,41:52.
[16]Macedo S,Bliebernicht M,Rocha A.Effect of two different nonpermeable cryoprotectants(NPC)on equine sperm viability after vitrification[J].Anim Reprod Sci,2018,194:18-19.
[17]Vidament M,Daire J M,Yvon J M,et al.Motility and fertility of stallion semen frozen with glycerol and/or dimethyl formamide[J].Theriogenology,2002,58:1-3.
[18]Alvarenga M A,Papa F O,Landim-Alvarenga F C,et al.Amides as cryoprotectants for freezing stallion semen:Areview[J].Anim Reprod Sci,2005,89:105-113.
[19]Melo,C,Alvarenga,M,Villaverde,A.I,et al.Comparison of different cryoprotectants on fertility of equine frozen semen[J].Biol Reprod,2007,77(1):178.
[20]Santiani A,Evangelista-Vargas S,Vargas S,et al.Cryopreservation of Peruvian Paso horse spermatozoa:dimethylacetamide preserved an optimal sperm function compared to dimethyl sulfoxide,ethylene glycol and glycerol[J].Andrologia,2016,49(6):1-6.
[21]Squires E L,Keith S L,Graham J K.Evaluation of alternative cryoprotectants for preserving stallion spermatozoa[J].Theriogenology,2004,62:1056-1065.
[22]Dietz J P,Sertich P L,Boston R C,et al.Comparison of ticarcillin and piperacillin in Kenney’s semen extender[J].Theriogenology,2007,68:848-852.
[23]Varner D D.The impact of cushioned centrifugation protocols on semen quality of stallions[J].Theriogenology,2012,77:1232-1239.
[24]Martins H S,Brito M F,Sampaio I B M,et al.Effects of antioxidants lactoferrin and catalase on stallion frozen semen[J].Reprod Fert Develop,2014,27(1):126-127.
[25]Vaez A,Mardani M,Razavi S.Impact of saffron on rat sperm membrane integrity and spermatogenesis status[J].Adv Biomed Res,2014,3:146-154.
[26]John A R,Baker M A.Oxidative stress and male reproductive biology[J].Reprod Fert Develop,2004,16:581-588.
[27]Bustamante F I C,Pederzolli C D,Sgaravatti A M,et al.Skim milk-egg yolk based semen extender compensates for nonenzymatic antioxidant activity loss during equine semen cryopreservation[J].Anim Reprod,2009,6(2):392-399.
[28]Christelle B,Peter S,Jean-Piere B.Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro[J].Mol Reprod Dev,2003,66:314-323.
[29]Franco J,Chaveiro A,Gois A,et al.Effects ofα-tocopherol and ascorbic acid on equine semen quality after cryopreservation[J].J Equine Vet Sci,2013,33(10):787-793.
[30]Wu Z,Zheng X,Luo Y,et al.Cryopreservation of stallion spermatozoa using different cryoprotectants and combinations of cryoprotectants[J].Anim Reprod Sci,2015,163:75-81.
[31]Flesch F M,Gadella B M.Dynamics of the mammalian sperm plasma membrane in the process of fertilization[J].Biochim Biophys Acta,2000,1469(3):197-235.
[32]Fang Y,Blair H,Zhong R,et al.Optimizing the freezing rate for ovine semen cryopreservation:Phospholipid profiles and functions of the plasma membrane and quality and fertilization of spermatozoa[J].Small Ruminant Res,2016,139:46-51.
[33]Papaa F O,Felício G B,Melo C M,et al.Replacing egg yolk with soybean lecithin in the cryopreservation of stallion semen[J].Anim Reprod Sci,2011,129:73-77.
[34]Parks J E,LynchD V.Lipid composition and thermotropic phase behavior of boar,bull,stallion,androoster sperm membranes[J].Cryobiology,1992,29:255-266.
[35]Daigneault B W,Graham J K,Bruemmer J E,et al.Cryopreservation of cooled semen and evaluation of sperm holding media for potential use in equine-assisted reproduction procedures[J].J Equine Vet Sci,2002,32(9):569-574.
[36]Linfor J,Pommer AC,Meyers S A.Osmotic stress induces tyrosine phosphorylation of equine sperm[J].Theriogenology,2002,58:355-358.
[37]Ball B A.Oxidative stress,osmotic stress and apoptosis:Impacts on sperm function and preservation in the horse[J].Anim Reprod Sci,2008,107:257-267.
[38]Sieme H,Harrison R A P,Petrunkina A M.Cryobiological determinants of frozen semen quality,with special reference to stallion[J].Anim Reprod Sci,2008,107:276-292.
[39]Muldrew K,McGann L E.Mechanisms of intracellular ice formation[J].Biophys J,1990,57(3):525-532.
[40]Freitas-Dell’Aqua C,Monteiro G A,Júnior J A,et al.The Effects of refrigeration temperature and storage time on apoptotic markers in equine semen[J].J Equine Vet Sci,2013,33:27-30.