甘草药材等级标准分析
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摘要
目的:通过对甘草药材的性状与主要化学成分指标进行分析,探索甘草药材性状与成分指标间的关联性,构建甘草药材的等级评价标准,为甘草药材的品质评价和质量控制提供参考。方法:将甘草药材外观性状指标与内在指标成分进行相关性分析、主成分分析和聚类分析,依据分析结果合理划分甘草药材等级并构建等级评价标准。结果:一等甘草药材:粗端直径> 1. 60 cm,中部围度> 3. 76 cm,内芯色度b*> 13. 88,外皮色度a*> 37. 61,甘草苷质量分数> 1. 13%,无虫蛀腐烂和杂质。二等甘草药材:粗端直径为1. 39～1. 60 cm,中部围度为3. 09～3. 76 cm,内芯色度b*为10. 22～13. 88,外皮色度a*为35. 57～37. 61,甘草苷质量分数0. 69%～1. 13%,无虫蛀腐烂和杂质。三等甘草药材:粗端直径为1. 08～1. 39 cm,中部围度2. 41～3. 09 cm,内芯色度b*为5. 16～10. 22,外皮色度a*为29. 19～35. 57,甘草苷质量分数为0. 55%～0. 69%,无虫蛀,无腐烂。等外甘草药材:粗端直径<1. 08 cm,中部围度<2. 41 cm,内芯色度b*<5. 16,外皮色度a*<29. 19,甘草苷质量分数<0. 55%。结论:该文建立的等级评价标准综合运用了外观、内在等多项指标,具有科学性、全面性、实用性等特点,可用于甘草药材的等级评价。
Objective: To explore the correlation between the characters and chemical constituents of Glycyrrhizae Radix et Rhizoma through the analysis of characters and main chemical constituents of Glycyrrhizae Radix et Rhizoma,and establish the grade evaluation criteria of Glycyrrhizae Radix et Rhizoma,so as to provide reference for the quality evaluation and quality control of Glycyrrhizae Radix et Rhizoma. Method: Correlation analysis,principal component analysis and cluster analysis were carried out for appearance and intrinsic indexes of Glycyrrhizae Radix et Rhizoma. Result: Class Ⅰ of Glycyrrhizae Radix et Rhizoma: the diameter of big end was more than 1. 60 cm, middle circumference was more than 3. 76 cm, inner core chromaticity b* was more than 13. 88,outer skin chromaticity a* was more than 37. 61,glycyrrhizin content was more than 1. 13%,with no worm,rot and impurity. Class Ⅱ of Glycyrrhizae Radix et Rhizoma: the diameter of big end was 1. 39-1. 60 cm,the middle circumference was 3. 09-3. 76 cm, the inner core chromaticity b* was 10. 22-13. 88, the skin chromaticity a* was 35. 57-37. 61,glycyrrhizin content was 0. 69%-1. 13%,with no worm,rot and impurity.Class Ⅲ of Glycyrrhizae Radix et Rhizoma: the diameter of the big end was 1. 08-1. 39 cm, the middle circumference was 2. 41-3. 09 cm,the inner core chromaticity b* was 5. 16-10. 22,the skin chromaticity a* was 29. 19-35. 57,glycyrrhizin content was 0. 55%-0. 69%,with no worm and rot. Substandard Glycyrrhizae Radix et Rhizoma: the diameter of the big end was lower than 1. 08 cm,the central circumference was lower than 2. 41 cm,the inner core color b* was lower than 5. 16,the outer skin color a* was lower than 29. 19,and glycyrrhizin content was lower than 0. 55%. Conclusion: The grade evaluation standards established in this paper include appearance and intrinsic indexes,with scientific,comprehensive and practical characteristics. It can be used for the grade evaluation of Glycyrrhizae Radix et Rhizoma medicinal materials.
Objective: To explore the correlation between the characters and chemical constituents of Glycyrrhizae Radix et Rhizoma through the analysis of characters and main chemical constituents of Glycyrrhizae Radix et Rhizoma,and establish the grade evaluation criteria of Glycyrrhizae Radix et Rhizoma,so as to provide reference for the quality evaluation and quality control of Glycyrrhizae Radix et Rhizoma. Method: Correlation analysis,principal component analysis and cluster analysis were carried out for appearance and intrinsic indexes of Glycyrrhizae Radix et Rhizoma. Result: Class Ⅰ of Glycyrrhizae Radix et Rhizoma: the diameter of big end was more than 1. 60 cm, middle circumference was more than 3. 76 cm, inner core chromaticity b* was more than 13. 88,outer skin chromaticity a* was more than 37. 61,glycyrrhizin content was more than 1. 13%,with no worm,rot and impurity. Class Ⅱ of Glycyrrhizae Radix et Rhizoma: the diameter of big end was 1. 39-1. 60 cm,the middle circumference was 3. 09-3. 76 cm, the inner core chromaticity b* was 10. 22-13. 88, the skin chromaticity a* was 35. 57-37. 61,glycyrrhizin content was 0. 69%-1. 13%,with no worm,rot and impurity.Class Ⅲ of Glycyrrhizae Radix et Rhizoma: the diameter of the big end was 1. 08-1. 39 cm, the middle circumference was 2. 41-3. 09 cm,the inner core chromaticity b* was 5. 16-10. 22,the skin chromaticity a* was 29. 19-35. 57,glycyrrhizin content was 0. 55%-0. 69%,with no worm and rot. Substandard Glycyrrhizae Radix et Rhizoma: the diameter of the big end was lower than 1. 08 cm,the central circumference was lower than 2. 41 cm,the inner core color b* was lower than 5. 16,the outer skin color a* was lower than 29. 19,and glycyrrhizin content was lower than 0. 55%. Conclusion: The grade evaluation standards established in this paper include appearance and intrinsic indexes,with scientific,comprehensive and practical characteristics. It can be used for the grade evaluation of Glycyrrhizae Radix et Rhizoma medicinal materials.
引文
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[12]陈小红,陈康,潘超美,等.化橘红药材商品规格等级标准分析[J].中国实验方剂学杂志,2017,23(11):23-28.
[13]翁德会,刘先琼,许腊英,等.不同等级厚朴饮片质量评价指标的系统聚类分析[J].中国实验方剂学杂志,2016,22(23):6-10.
[2]任玲.甘草有效成分的药理活性研究[J].生物技术世界,2016(5):227.
[3]姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017,31(7):25-28.
[4]中华人民共和国卫生部,国家医药管理局.七十六种药材商品规格标准[M]. 1984:4.
[5]邓哲,章军,焦梦姣,等.以质量常数为核心的黄芩饮片等级评价研究[J].中国中药杂志,2017,42(9):1673-1678.
[6]邓哲,焦梦姣,章军,等.相对质量常数用于甘草饮片等级评价研究[J].中国中药杂志,2017,42(13):2492-2496.
[7]曹雪,蔡广知,赵凌,等.基于复合质量常数的北柴胡饮片等级评价[J].中国实验方剂学杂志,2018,24(18):36-41.
[8]康传志,周涛,江维克,等.根类药材商品规格等级标准研究模式探讨[J].中国中药杂志,2016,41(5):769-775.
[9]刘大会,徐娜,郭兰萍,等.三七药材质量特征和商品规格等级标准研究[J].中国中药杂志,2016,41(5):776-785.
[10]邹慧琴,李硕,林相龙,等.基于色度学理论的甘草颜色数字化方法学研究[J].世界科学技术—中医药现代化,2014,16(12):2681-2685.
[11]曹旭林,徐昌艳,邓强,等.商品天冬等级与其活性物质含量的相关性分析[J].中国实验方剂学杂志,2017,23(22):55-59.
[12]陈小红,陈康,潘超美,等.化橘红药材商品规格等级标准分析[J].中国实验方剂学杂志,2017,23(11):23-28.
[13]翁德会,刘先琼,许腊英,等.不同等级厚朴饮片质量评价指标的系统聚类分析[J].中国实验方剂学杂志,2016,22(23):6-10.