微藻自絮凝采收工艺研究
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摘要
[目的]研究微藻低成本采收工艺。[方法]通过对微藻表面电荷与细胞自絮凝关系的研究,获得细胞自絮凝的机理。明确pH、微藻养殖深度、微藻细胞浓度和日辐射量对微藻细胞表面的影响,分析影响试验结果的主要因素,总结出微藻自絮凝条件。[结果]在750 nm处吸光度(OD_(750nm))大于5.0、液层深度2 cm、日辐射量16.2~28.1 MJ/(m~2·d)时,3~5 h均可实现自絮凝沉降,沉降率均在75%以上。[结论]该研究可为微藻低成本自絮凝采收提供理论依据。
[Objective] The research aimed to study the process of microalgae harvesting at low cost.[Method]The relationship between cell surface charge and cell aggregation was studied,and the mechanism of microalgal cell aggregation was obtained.The effects of pH,microalgae culture depth,microalgal cell concentration and daily radiation on the surface charge of microalgae were determined.By analyzing the main factors affecting the experimental results,the flocculating conditions of microalgae were summarized.[Result] When OD_(750 nm)was greater than 5,the depth of liquid layer was 2 cm,and the daily radiation was 16.2-28.1 MJ/( m~2·d),the cell aggregation and sedimentation occurred after the reaction time was 3-5 hours.The settlement rate was above 75%. [Conclusion]This study can provide a theoretical basis for low cost harvesting of microalgae.
[Objective] The research aimed to study the process of microalgae harvesting at low cost.[Method]The relationship between cell surface charge and cell aggregation was studied,and the mechanism of microalgal cell aggregation was obtained.The effects of pH,microalgae culture depth,microalgal cell concentration and daily radiation on the surface charge of microalgae were determined.By analyzing the main factors affecting the experimental results,the flocculating conditions of microalgae were summarized.[Result] When OD_(750 nm)was greater than 5,the depth of liquid layer was 2 cm,and the daily radiation was 16.2-28.1 MJ/( m~2·d),the cell aggregation and sedimentation occurred after the reaction time was 3-5 hours.The settlement rate was above 75%. [Conclusion]This study can provide a theoretical basis for low cost harvesting of microalgae.
引文
[1]VERGINI S,ARAVANTINOU A F,MANARIOTIS I D.Harvesting of freshwater and marine microalgae by common flocculants and magnetic microparticles[J].Journal of applied phycology,2016,28(2):1041-1049.
[2]VANDAMME D,FOUBERT I,MUYLAERT K.Flocculation as a low-cost method for harvesting microalgae for bulk biomass production[J].Trends in biotechnology,2013,31(4):233-239.
[3]樊华,韩佩,王菁晗,等.微藻生物采收技术的现状和展望[J].生物学杂志,2017,34(2):26-32.
[4]张海阳,匡亚莉,林喆.能源微藻采收技术研究进展[J].化工进展,2013,32(9):2092-2098.
[5]SALIM S,KOSTERINK N R,TCHETKOUA WACKA N D,et al.Mechanism behind autoflocculation of unicellular green microalgae Ettlia texensis[J].Journal of biotechnology,2014,174:34-38.
[6]SALIM S,BOSMA R,VERMUEM H,et al.Harvesting of microalgae by bio-flocculation[J].Journal of applied phycology,2011,23(5):849-855.
[7]ALAM M A,WAN C,GUO S L,et al.Characterization of the flocculating agent from the spontaneously flocculating microalga Chlorella vulgaris JSC-7[J].Journal of bioscience and bioengineering,2014,118(1):29-33.
[8]万春,张晓月,赵心清,等.利用絮凝进行微藻采收的研究进展[J].生物工程学报,2015,31(2):161-171.
[2]VANDAMME D,FOUBERT I,MUYLAERT K.Flocculation as a low-cost method for harvesting microalgae for bulk biomass production[J].Trends in biotechnology,2013,31(4):233-239.
[3]樊华,韩佩,王菁晗,等.微藻生物采收技术的现状和展望[J].生物学杂志,2017,34(2):26-32.
[4]张海阳,匡亚莉,林喆.能源微藻采收技术研究进展[J].化工进展,2013,32(9):2092-2098.
[5]SALIM S,KOSTERINK N R,TCHETKOUA WACKA N D,et al.Mechanism behind autoflocculation of unicellular green microalgae Ettlia texensis[J].Journal of biotechnology,2014,174:34-38.
[6]SALIM S,BOSMA R,VERMUEM H,et al.Harvesting of microalgae by bio-flocculation[J].Journal of applied phycology,2011,23(5):849-855.
[7]ALAM M A,WAN C,GUO S L,et al.Characterization of the flocculating agent from the spontaneously flocculating microalga Chlorella vulgaris JSC-7[J].Journal of bioscience and bioengineering,2014,118(1):29-33.
[8]万春,张晓月,赵心清,等.利用絮凝进行微藻采收的研究进展[J].生物工程学报,2015,31(2):161-171.