应用激光散斑血流成像技术观察电针对心包经线上微循环血流灌注量的影响
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摘要
目的:比较电针内关穴前后心包经线上浅表微循环血流灌注量(MBPU)的差异,探讨电针对其影响。方法:应用激光散斑血流成像系统,观察18名健康志愿者电针前和电针时心包经线上曲泽穴及其旁开非经非穴对照点、心包线上两个非穴位点浅表MBPU。结果:常态下志愿者心包经线上曲泽穴浅表MBPU高于其旁开非经非穴对照点(P<0.01);电针内关穴时,心包经线上曲泽穴和两个非穴位点MBPU均下降(P<0.05,P<0.01),曲泽穴旁开非经非穴对照点MBPU仍低于曲泽穴(P<0.01)。结论:电针对心包经线浅表MBPU有调节作用,且有一定穴位特异性。
Objective: To investigate the difference in the superficial microcirculation blood perfusion units(MBPU) on pericardium meridian before and after electro-acupuncture(EA) on Neiguan(PC6), and to explore how EA act in the MBPU. Methods: Eighteen volunteers were included into this study. Full-Field laser perfusion imager system was used to detect the superficial MBPU on Quze(PC3), its bilateral control points and other two points of pericardium meridian. Results: Before EA, the MBPU of Quze(PC3) was significantly higher than that of its bilateral control points(P<0.01). After EA on Neiguan(PC6), the MBPU along the pericardium meridian decreased significantly(P<0.05, P<0.01). And the MBPU of Quze(PC3) was still higher than that of its bilateral control points(P<0.01). Conclusion: EA has a regulatory effect on the superficial MBPU of pericardium meridian, and it has certain acupoint specificity.
Objective: To investigate the difference in the superficial microcirculation blood perfusion units(MBPU) on pericardium meridian before and after electro-acupuncture(EA) on Neiguan(PC6), and to explore how EA act in the MBPU. Methods: Eighteen volunteers were included into this study. Full-Field laser perfusion imager system was used to detect the superficial MBPU on Quze(PC3), its bilateral control points and other two points of pericardium meridian. Results: Before EA, the MBPU of Quze(PC3) was significantly higher than that of its bilateral control points(P<0.01). After EA on Neiguan(PC6), the MBPU along the pericardium meridian decreased significantly(P<0.05, P<0.01). And the MBPU of Quze(PC3) was still higher than that of its bilateral control points(P<0.01). Conclusion: EA has a regulatory effect on the superficial MBPU of pericardium meridian, and it has certain acupoint specificity.
引文
[1]Barcelos A,Lamas C,Tibiri?a E.Evaluation of microvascular endothelial function in patients with infective endocarditis using laser speckle contrast imaging and skin video-capillaroscopy:research proposal of a case control prospective study.Bmc Res Notes,2017,10(1):342-347
[2]Humeau-Heurtier A,Abraham P,Mahe G.Analysis of laser speckle contrast images variability using a novel empirical mode decomposition:comparison of results with laser Doppler flowmetry signals variability.IEEE Trans Med Imaging,2015,34(2):618-627
[3]薛艳,吴松,李佳,等.针刺内关穴对心肌缺血中枢神经调节作用的研究进展.中国中医基础医学杂志,2017,23(4):592-594
[4]张小艳,熊鹏,樊亚崑,等.针灸内关、灵道及曲泽穴治疗心律失常的临床研究.实用中西医结合临床,2016,16(11):16-18
[5]修春英,董亚琴,许金森.电针曲泽穴对急性缺氧心功能作用的相对特异性研究.中华中医药杂志,2014,29(6):2015-2017
[6]董亚琴,修春英,朱小香,等.针刺内关对急性缺氧健康志愿者心包经经皮氧分压的影响.环球中医药,2014,7(1):21-23
[7]Fercher A F,Briers J D.Flow visualization by means of singleexposure speckle photography.Optics Communications,1981,37(5):326-330
[8]孔平,杨晖,郑刚,等.激光散斑血流成像技术研究新进展.光学技术,2014,40(1):21-26
[9]O’Doherty J,Mcnamara P,Clancy N T,et al.Comparison of instruments for investigation of microcirculatory blood flow and red blood cell concentration.J Biomed Opt,2009,14(3):034025
[10]Oltean M,Aneman A,Dindelegan G,et al.Monitoring of the intestinal mucosal perfusion using laser Doppler flowmetry after multivisceral transplantation.Transplant Proc,2005,37(8):3323-3324
[11]田宇瑛,黄涛,王广军,等.应用激光散斑技术观察针刺合谷对面部不同区域血流的影响.中国中医基础医学杂志,2013,19(2):183-184
[12]Li Y,Li X,Zhou D,et al.Microcirculation perfusion monitor on the back of the health volunteers.Evid Based Complement Alternat Med,2013,2013:590698
[13]Wang S,Dong Zhang,Limei Tang,et al.Effects of electroacupuncture stimulation at“Zusanli”acupoint on hepatic NO release and blood perfusion in mice.Evid Based Complement Alternat Med,2015,2015:826805
[14]宋晓晶,张栋,马慧敏,等.电针对小鼠肝脏血流灌注量影响的激光散斑成像显示.激光生物学报,2011,20(1):15-21
[15]林丽娇,许金森,朱小香,等.针灸影响微循环的研究进展.中国针灸,2015,35(2):203-208
[16]贾亚威,杨晖,李然,等.激光散斑血流成像对中医理疗功效的检测.光学精密工程,2017,25(6):1410-1417
[17]傅汉菁,刘秀华,薛全福.中国病理生理学会微循环专业委员会第十一届学术会议纪要.微循环学杂志,2006,16(4):1-2,6
[18]许金森,郑淑霞,潘晓华,等.阴虚阳虚体质任脉线上微循环血流灌注量的比较及电针对其影响.中华中医药杂志,2016,31(10):3992-3994
[19]陈铭,吴祖星,许金森,等.大肠经穴与非穴的氧分压和微循环血流灌注量的比较及针刺的影响.中华中医药杂志,2012,27(12):3198-3201
[20]Zheng S X,Pan X H,Xu J S,et al.Variations in energy metabolism along the pericardium meridian and its relationship with visceral function adjustments during electroacupuncture.BMC Complement Altern Med,2014,14:323-329
[21]杨宗保,沈佳成,王亚东,等.应用1H NMR代谢组技术研究电针治疗胃溃疡的中枢响应机制.中华中医药杂志,2017,32(7):3191-3195
[22]徐东升,佘琛,王佳,等.大鼠“太冲”穴区皮肤组织中血管和神经纤维的显微结构特征.针刺研究,2016,41(6):486-491
[23]Shinbara H,Okubo M,Kimura K,et al.Participation of calcitonin gene related peptide released via axon reflex in the local increase in muscle blood flow following manual acupuncture.Acupunct Med,2013,31(1):81-87
[24]Choi W,Lee S,Cho S,et al.Differential autonomic response to acupuncture at wood and metal of five-shu acupoints.J Altern Complement Med,2012,18(10):959-964
[25]刘里远,张慧,潘娟.皮肤交感物质分布线的发现及其与中医经络实质的关系.中国针灸,2003,23(1):23-26
[2]Humeau-Heurtier A,Abraham P,Mahe G.Analysis of laser speckle contrast images variability using a novel empirical mode decomposition:comparison of results with laser Doppler flowmetry signals variability.IEEE Trans Med Imaging,2015,34(2):618-627
[3]薛艳,吴松,李佳,等.针刺内关穴对心肌缺血中枢神经调节作用的研究进展.中国中医基础医学杂志,2017,23(4):592-594
[4]张小艳,熊鹏,樊亚崑,等.针灸内关、灵道及曲泽穴治疗心律失常的临床研究.实用中西医结合临床,2016,16(11):16-18
[5]修春英,董亚琴,许金森.电针曲泽穴对急性缺氧心功能作用的相对特异性研究.中华中医药杂志,2014,29(6):2015-2017
[6]董亚琴,修春英,朱小香,等.针刺内关对急性缺氧健康志愿者心包经经皮氧分压的影响.环球中医药,2014,7(1):21-23
[7]Fercher A F,Briers J D.Flow visualization by means of singleexposure speckle photography.Optics Communications,1981,37(5):326-330
[8]孔平,杨晖,郑刚,等.激光散斑血流成像技术研究新进展.光学技术,2014,40(1):21-26
[9]O’Doherty J,Mcnamara P,Clancy N T,et al.Comparison of instruments for investigation of microcirculatory blood flow and red blood cell concentration.J Biomed Opt,2009,14(3):034025
[10]Oltean M,Aneman A,Dindelegan G,et al.Monitoring of the intestinal mucosal perfusion using laser Doppler flowmetry after multivisceral transplantation.Transplant Proc,2005,37(8):3323-3324
[11]田宇瑛,黄涛,王广军,等.应用激光散斑技术观察针刺合谷对面部不同区域血流的影响.中国中医基础医学杂志,2013,19(2):183-184
[12]Li Y,Li X,Zhou D,et al.Microcirculation perfusion monitor on the back of the health volunteers.Evid Based Complement Alternat Med,2013,2013:590698
[13]Wang S,Dong Zhang,Limei Tang,et al.Effects of electroacupuncture stimulation at“Zusanli”acupoint on hepatic NO release and blood perfusion in mice.Evid Based Complement Alternat Med,2015,2015:826805
[14]宋晓晶,张栋,马慧敏,等.电针对小鼠肝脏血流灌注量影响的激光散斑成像显示.激光生物学报,2011,20(1):15-21
[15]林丽娇,许金森,朱小香,等.针灸影响微循环的研究进展.中国针灸,2015,35(2):203-208
[16]贾亚威,杨晖,李然,等.激光散斑血流成像对中医理疗功效的检测.光学精密工程,2017,25(6):1410-1417
[17]傅汉菁,刘秀华,薛全福.中国病理生理学会微循环专业委员会第十一届学术会议纪要.微循环学杂志,2006,16(4):1-2,6
[18]许金森,郑淑霞,潘晓华,等.阴虚阳虚体质任脉线上微循环血流灌注量的比较及电针对其影响.中华中医药杂志,2016,31(10):3992-3994
[19]陈铭,吴祖星,许金森,等.大肠经穴与非穴的氧分压和微循环血流灌注量的比较及针刺的影响.中华中医药杂志,2012,27(12):3198-3201
[20]Zheng S X,Pan X H,Xu J S,et al.Variations in energy metabolism along the pericardium meridian and its relationship with visceral function adjustments during electroacupuncture.BMC Complement Altern Med,2014,14:323-329
[21]杨宗保,沈佳成,王亚东,等.应用1H NMR代谢组技术研究电针治疗胃溃疡的中枢响应机制.中华中医药杂志,2017,32(7):3191-3195
[22]徐东升,佘琛,王佳,等.大鼠“太冲”穴区皮肤组织中血管和神经纤维的显微结构特征.针刺研究,2016,41(6):486-491
[23]Shinbara H,Okubo M,Kimura K,et al.Participation of calcitonin gene related peptide released via axon reflex in the local increase in muscle blood flow following manual acupuncture.Acupunct Med,2013,31(1):81-87
[24]Choi W,Lee S,Cho S,et al.Differential autonomic response to acupuncture at wood and metal of five-shu acupoints.J Altern Complement Med,2012,18(10):959-964
[25]刘里远,张慧,潘娟.皮肤交感物质分布线的发现及其与中医经络实质的关系.中国针灸,2003,23(1):23-26