不同方法提取地榆总三萜含量的比较及其抗氧化活性研究
|
摘要
为进一步开发利用地榆资源,采用水煮醇沉法、回流提取法和浸渍法对地榆总三萜进行提取,并以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法对地榆总三萜的抗氧化活性进行评价。结果表明,3种提取方法所得地榆总三萜含量依次为:回流提取法(88.66 mg/g)>浸渍法(55.26 mg/g)>水煮醇沉法(44.31 mg/g)。地榆总三萜和V_C对DPPH自由基的半数清除率IC_(50)分别是3.04、5.46 g/mL。采用回流提取法得到的地榆总三萜含量最高,且回流提取法提取得到的地榆总三萜对于DPPH自由基的清除能力优于抗氧化剂V_C,具有较强的抗氧化活性。
To provide scientific basis for further development resources of Sanguisorba officinalis,The total triterpenes of Sanguisorba officinalis were extracted by hot water extraction and ethanel precipitation,reflux extraction and impregnation method. And antioxidant activities of total triterpenes from Sanguisorba officinalis were evaluated by 1,1-diphenyl-2-trinitrophenylhydrazine method. The result indicated that the content of total triterpene from Sanguisorba officinalis obtained by the three extraction methods was as fallows:reflux extraction(88.66 mg/g)> impregnation(55.26 mg/g)> hot water extraction and ethanel precipitation(44.31 mg/g).The total triterpenes of Sanguisorba officinalis and vitamin C had the DPPH radical scavenging ability with IC_(50) of 3.04 g/mL and 5.46 g/mL. The content of total triterpenoids from Sanguisorba officinalis was the highest by reflux extraction,and the scavenging ability of total triterpenoids from Sanguisorba officinalis by reflux extraction had more antioxidant activity compared with the commonly used antioxidant vitamin C. Moreover,the total flavonoids content from leaves had more antioxidant activity compared with the commonly used antioxidant vitamin C.
To provide scientific basis for further development resources of Sanguisorba officinalis,The total triterpenes of Sanguisorba officinalis were extracted by hot water extraction and ethanel precipitation,reflux extraction and impregnation method. And antioxidant activities of total triterpenes from Sanguisorba officinalis were evaluated by 1,1-diphenyl-2-trinitrophenylhydrazine method. The result indicated that the content of total triterpene from Sanguisorba officinalis obtained by the three extraction methods was as fallows:reflux extraction(88.66 mg/g)> impregnation(55.26 mg/g)> hot water extraction and ethanel precipitation(44.31 mg/g).The total triterpenes of Sanguisorba officinalis and vitamin C had the DPPH radical scavenging ability with IC_(50) of 3.04 g/mL and 5.46 g/mL. The content of total triterpenoids from Sanguisorba officinalis was the highest by reflux extraction,and the scavenging ability of total triterpenoids from Sanguisorba officinalis by reflux extraction had more antioxidant activity compared with the commonly used antioxidant vitamin C. Moreover,the total flavonoids content from leaves had more antioxidant activity compared with the commonly used antioxidant vitamin C.
引文
[1]李时珍.本草纲目[M].重庆:重庆出版社,2006:185
[2]张向阳,赵如同,刘春燕,等.地榆、地榆炭对小鼠止血作用影响的实验研究[J].河北中医,2017,39(5):735-738
[3]Zhu Xue,Wang Ke,Zhang Kai,et al.Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway[J].Int J Mol,2013,14(9):18041-18055
[4]Park K H,Koh D,Kim K,et al.Antiallergic activity of a disaccharide isolated from Sanguisorba officinalis[J].phytother Res,2004,18(8):658-662
[5]陈红宇,沈洪.地榆活性成分及抗炎作用研究进展[J].中医药导报,2017,23(17):110-113
[6]张熙祎.地榆升白片治疗白细胞减少疗效观察及体会[J].陕西中医,2016,37(4):470
[7]赵杨,叶冲,毛寒冰,等.响应面法优化地榆总黄酮提取工艺[J].广州化工,2017,45(24):111-114
[8]毛迪锐,姜贵全,张诗朦,等.地榆多酚分离纯化及其抗氧化性研究[J].食品工业科技,2015(21):68-72
[9]代良敏,熊永爱,范奎,等.地榆化学成分与药理作用研究进展[J].中国实验方剂学杂志,2016,22(20):189-195
[10]秦晶晶,李齐激,薛琰,等.刺梨总三萜提取方法及其α-葡萄糖苷酶抑制活性研究[J].食品工业科技,2014(10):186-189
[11]张爽,任亚梅,刘春利,等.响应面试验优化苹果渣总三萜超声提取工艺[J].食品科学,2015,36(16):44-50
[12]林水花,吴建国,郑燕枝,等.响应面法优化超声提取毛花猕猴桃根中三萜类化合物的工艺研究[J].天然产物研究与开发,2014,26(3):431-437
[13]曾智,徐恒,白波,等.柠条锦鸡儿不同极性部位抗氧化及抗须癣毛癣菌活性初筛[J].天然产物研究与开发,2017,29(8):1362-1367
[14]刘晓艳,陈艺煊,吴林秀,等.响应面法优化灵芝总三萜酶辅助提取工艺及其抗氧化活性研究[J].食品科技,2017,42(8):225-230
[2]张向阳,赵如同,刘春燕,等.地榆、地榆炭对小鼠止血作用影响的实验研究[J].河北中医,2017,39(5):735-738
[3]Zhu Xue,Wang Ke,Zhang Kai,et al.Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway[J].Int J Mol,2013,14(9):18041-18055
[4]Park K H,Koh D,Kim K,et al.Antiallergic activity of a disaccharide isolated from Sanguisorba officinalis[J].phytother Res,2004,18(8):658-662
[5]陈红宇,沈洪.地榆活性成分及抗炎作用研究进展[J].中医药导报,2017,23(17):110-113
[6]张熙祎.地榆升白片治疗白细胞减少疗效观察及体会[J].陕西中医,2016,37(4):470
[7]赵杨,叶冲,毛寒冰,等.响应面法优化地榆总黄酮提取工艺[J].广州化工,2017,45(24):111-114
[8]毛迪锐,姜贵全,张诗朦,等.地榆多酚分离纯化及其抗氧化性研究[J].食品工业科技,2015(21):68-72
[9]代良敏,熊永爱,范奎,等.地榆化学成分与药理作用研究进展[J].中国实验方剂学杂志,2016,22(20):189-195
[10]秦晶晶,李齐激,薛琰,等.刺梨总三萜提取方法及其α-葡萄糖苷酶抑制活性研究[J].食品工业科技,2014(10):186-189
[11]张爽,任亚梅,刘春利,等.响应面试验优化苹果渣总三萜超声提取工艺[J].食品科学,2015,36(16):44-50
[12]林水花,吴建国,郑燕枝,等.响应面法优化超声提取毛花猕猴桃根中三萜类化合物的工艺研究[J].天然产物研究与开发,2014,26(3):431-437
[13]曾智,徐恒,白波,等.柠条锦鸡儿不同极性部位抗氧化及抗须癣毛癣菌活性初筛[J].天然产物研究与开发,2017,29(8):1362-1367
[14]刘晓艳,陈艺煊,吴林秀,等.响应面法优化灵芝总三萜酶辅助提取工艺及其抗氧化活性研究[J].食品科技,2017,42(8):225-230