液相色谱-串联质谱法测定烟叶中的玉米赤霉烯酮
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摘要
建立了液相色谱-串联质谱法测定烟叶中玉米赤霉烯酮的方法。样品经无水乙醚提取,正己烷净化,采用多反应检测扫描方式(MRM),ESI负离子模式,确定了玉米赤霉烯酮的分子离子峰为(m/z)317.30,定量离子为(m/z)175.15,定性离子为(m/z)131.10,273.25。玉米赤霉烯酮在0～10ng/mL范围内线性关系良好(R>0.999),方法检出限为0.42μg/kg;平均回收率为96.4%～101.1%,日内精密度RSD为2.5%,日间精密度RSD为9.05%。采用该方法满足烟叶中玉米赤霉烯酮的低含量检测。
A method for the determination of zearalenone in tobacco leaves by LC/MS was established. The sample was extracted with anhydrous ether and purified with n-hexane, and through the scanning mode of MRM and the negative ion mode of ESI, the molecular ion peak of zearalenone,quantitative ions and qualitative ions was respectively determined to be(m/z) 317.30,(m/z) 175.15 and(m/z) 131.10, 273.25. The linear relationship of zearalenone was good in the range of 0～10 ng/mL(R>0.999), the detection limit was 0.42 μg/kg. The average recovery was 96.4%～101.1%, the intra-day precision RSD was 2.5% and the day precision RSD was 9.05%. The method is suitable for low content detection of zearalenone in tobacco leaves.
A method for the determination of zearalenone in tobacco leaves by LC/MS was established. The sample was extracted with anhydrous ether and purified with n-hexane, and through the scanning mode of MRM and the negative ion mode of ESI, the molecular ion peak of zearalenone,quantitative ions and qualitative ions was respectively determined to be(m/z) 317.30,(m/z) 175.15 and(m/z) 131.10, 273.25. The linear relationship of zearalenone was good in the range of 0～10 ng/mL(R>0.999), the detection limit was 0.42 μg/kg. The average recovery was 96.4%～101.1%, the intra-day precision RSD was 2.5% and the day precision RSD was 9.05%. The method is suitable for low content detection of zearalenone in tobacco leaves.
引文
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[7]朱文倩,黄健花,金青哲,等.高效液相色谱法定量检测玉米油中玉米赤霉烯酮的溶剂萃叐预处理方法研究[J].中国油脂,2018,43(6):140-143.
[8]林有裕.高效液相色谱法测定玉米赤霉烯酮的方法[J].现代食品,2016,(20):106-107.
[9]王丹,姚婷,甘卓亭,等.超高效液相色谱-四极杄-飞行时间质谱法检测茶叶中玉米赤霉烯酮[J].化学试剂,2019,41(2):162-167.
[10]王巍,崔勇,张冝英,等.超高效液相色谱-串联质谱法测定玉米油中玉米赤酶烯酮的残留量[J].食品安全质量检测学报, 2016, 7(5):2066-2070.
[11]王浩宇,孙晶,常晓娇,等.玉米赤霉烯酮降解酶ZLHY6的包埋及稳定性研究[J].粮油食品科技,2018,26(2):63-67.
[12]刘刚,韩铮,聂冬霞,等.玉米赤霉烯酮快速间接竞争酶联免疫检测技术研究及应用[J].上海农业学报,2015,31(1):92-95.
[13]刘涛,王向红,常彧,等.直接竞争酶联免疫法检测大麦中玉米赤霉烯酮[J].食品与机械,2008,24(2):97-99,117.
[14]熊挺,蒋蔚,韩阳瑞,等.家禽饲料中玉米赤霉烯酮化学収光酶联免疫分析方法的建立[J].畜牧与兽医,2018,50(2):122-130.