红细胞磁化技术用于献血者不规则抗体筛查的试验研究
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摘要
目的探讨红细胞磁化技术(EMT)用于献血者不规则抗体筛查的可行性。方法采用基于EMT的柯利斯全自动血型系统结合间接抗人球蛋白试验对上海地区4 995例献血者标本进行不规则抗体筛查,并与微孔板木瓜酶法作比对试验。结果柯利斯系统初筛反应性22例(4.4‰),微孔板木瓜酶法初筛反应性53例(10.6‰),经参比实验室确认阳性为10例。结论基于EMT的柯利斯全自动血型系统作不规则抗体筛查,具有操作方便、灵敏度高、结果准确、完全溯源等特点。
Objective Investigate the feasibility of using erythrocyte magnetization technology for irregular antibody screening of blood donors.Methods A total of 4 995 samples from blood donors in Shanghai was screened for irregular antibodies by COLIS automatic blood group system combined with indirect anti-human globulin test based on RBC magnetization technology,and the test was compared with papain microplate method.Results Among the 4 995 blood donors,22 cases(4.4‰)were initially screening reactive for COLIS system,while 53 cases(10.6‰)were initially screening reactivity by microplate papain method.Conclusion The COLIS automatic blood group system based on red blood cell magnetization technology was characterized by easy operation,high sensitivity,accurate results and complete traceability.
Objective Investigate the feasibility of using erythrocyte magnetization technology for irregular antibody screening of blood donors.Methods A total of 4 995 samples from blood donors in Shanghai was screened for irregular antibodies by COLIS automatic blood group system combined with indirect anti-human globulin test based on RBC magnetization technology,and the test was compared with papain microplate method.Results Among the 4 995 blood donors,22 cases(4.4‰)were initially screening reactive for COLIS system,while 53 cases(10.6‰)were initially screening reactivity by microplate papain method.Conclusion The COLIS automatic blood group system based on red blood cell magnetization technology was characterized by easy operation,high sensitivity,accurate results and complete traceability.
引文
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[12]秦建江,杜平,何智.Rh血型免疫性抗体致溶血性输血反应的探讨[J].新疆医学,2012,42(7):63-65.
[13]赵莉.输血前进行不规则抗体检验的临床意义和实际应用价值[J/CD].世界最新医学信息文摘(电子版),2016,16(47):158.
[2]张雄民,向东,孟妍,等.上海地区部分献血者中不规则抗体的调查[J].中国医药导刊,2009,11(4):647-648.
[3]陈倩,张志亮,唐秋萍.海南地区无偿献血者不规则抗体筛查结果分析[J].中国输血杂志,2015,28(4):416-418.
[4]傅立强,朱守兵,刘祎.红细胞磁化技术在血型检测、不规则抗体筛查中的应用[J].中国卫生检验杂志,2017,27(12):1737-1739.
[5]蔡茵,曹斌,周喆,等.上海地区无偿献血者血液中不规则抗体的调查[J].中国输血杂志,2017,30(7):791-793.
[6]GARCA M A,BAUTISTA L,PALOMINO F.Should blood donors be routinely screened for irregular antibodies?[J].Immunohematology,2012,28(2):60-67.
[7]王志红.4种方法检测红细胞血型IgG类抗体敏感性比较[J].临床血液学杂志(输血与检验版),2012,25(4):511-512.
[8]马曙轩,白丽霞,刘景汉,等.4种检测IgG红细胞抗体方法的比较[J].第三军医大学学报,2005,27(7):660-662.
[9]孙爱农,许惠根,吴晓燕,等.三种红细胞不规则抗体筛选试验方法的比较[J].检验医学,2007,22(1):71-74.
[10]纪勇平,赵龙友,周斌.不规则抗体对输血的影响[J].中国预防医学杂志,2015,16(8):620-623.
[11]孔文兵,赖福才,彭道波,等.红细胞血型不规则抗体与安全输血[J].中国输血杂志,2014,27(9):929-930.
[12]秦建江,杜平,何智.Rh血型免疫性抗体致溶血性输血反应的探讨[J].新疆医学,2012,42(7):63-65.
[13]赵莉.输血前进行不规则抗体检验的临床意义和实际应用价值[J/CD].世界最新医学信息文摘(电子版),2016,16(47):158.