桂北果蔗甘蔗杆状病毒的分子检测与分析
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摘要
从桂北主栽果蔗区采集表现褪绿斑点或花叶及无症状的未脱毒拔地拉、青皮果蔗、紫皮果蔗、罗汉果蔗、广东黄皮、同安果蔗及脱毒拔地拉等7个品种蔗叶样品88份,用SCBV检测引物对采集的样品进行PCR检测分析。检测结果显示:有2份为SCBV阳性,检出率2.3%,均为未脱毒果蔗拔地拉,其中1份叶片无明显的症状;脱毒拔地拉、青皮果蔗、紫皮果蔗、罗汉果蔗、广东黄皮、同安果蔗检测均为阴性。表明桂北果蔗区存在SCBV侵染。
In order to detection and analysis the samples by PCR with SCBV detection primers,a total of 88 fruit cane leaf samples including 7 fruit cane varieties(undetoxified Badilla,green peel sugarcane,virus-free Badilla,purple peel sugarcane,Luohan sugarcane,Guangdong yellow peel species,Tongan fruit sugar)with chlorosis spots and mottle,and without symptoms were collected from main fruit cane areas in northern Guangxi.The results showed that,among 88 tested samples,SCBV was found in 2 samples,which indicated the positive rate were up to 2.3%.That 2 samples were undetoxified Badilla,and one of them showed no obvious symptoms,while the other 6 varieties(virus-free Badilla,green peel sugarcane,purple peel sugarcane,Luohan sugarcane,Guangdong yellow peel species,Tongan fruit sugar)were all found to be SCBV negative.The results indicated that there was SCBV infection in the fruit cane area of Northern Guangxi.
In order to detection and analysis the samples by PCR with SCBV detection primers,a total of 88 fruit cane leaf samples including 7 fruit cane varieties(undetoxified Badilla,green peel sugarcane,virus-free Badilla,purple peel sugarcane,Luohan sugarcane,Guangdong yellow peel species,Tongan fruit sugar)with chlorosis spots and mottle,and without symptoms were collected from main fruit cane areas in northern Guangxi.The results showed that,among 88 tested samples,SCBV was found in 2 samples,which indicated the positive rate were up to 2.3%.That 2 samples were undetoxified Badilla,and one of them showed no obvious symptoms,while the other 6 varieties(virus-free Badilla,green peel sugarcane,purple peel sugarcane,Luohan sugarcane,Guangdong yellow peel species,Tongan fruit sugar)were all found to be SCBV negative.The results indicated that there was SCBV infection in the fruit cane area of Northern Guangxi.
引文
[1] 孙生仁,张慧丽,吴小斌,等.甘蔗杆状病毒及其启动子功能的研究进展[J].植物生理学报,2018,54(6):943-950.
[2] Autrey LJC,Boolell LJC,Jones S,et al.The distribution of sugarcane bacilliform virus in various geographical regions[J].Proc Int Soc Sugar Cane Technology,1995,21:527-541.
[3] Lockhart BEL,Autrey LJC.Sugarcane bacilliform virus[C]//Rott P,Bailey RA,Comstock JC,et al.A Guide to Sugarcane Diseases.Montpellier:CI-RAD-ISSCT Press,2000:268-272.
[4] Karuppaiah R,Viswanathan R,Kumar VG.Genetic diversity of sugarcane bacilliform virus isolates infecting Saccharum spp.in India[J].Virus Genes,2013,46(3):505-516.
[5] 蔡艳清,许东林,周国辉,等.广东首次发现甘蔗杆状病毒[C]//中国植物病理学会.中国植物病理学会2004年学术年会论文集.北京:中国农业科学技术出版社,2004:213-215.
[6] 徐正银,周龙武,唐瑶,等.广西甘蔗杆状病毒的分子检测及分布调查[J].南方农业学报,2015,46(11):1980-1984.
[7] 李文凤,黄应昆,姜冬梅,等.云南甘蔗杆状病毒的分子检测及其对甘蔗品质和产量的影响[J].植物病理学报,2010,40(6):651-654.
[8] 李瑞美,何炎森.不同类型果蔗品种资源氨基酸等营养成分分析[J].热带作物学报,2017,38(1):171-174.
[9] 余坤兴,谭芳,李松,等.广西果蔗生产现状与健康发展对策[J].广西蔗糖,2007(4):14-20.
[10] Seifer DL,Salomon R,Marie JV,et al.Characterization of a novel potyvirus isolated from maize in Israel[J].Phytopathology,2000,90(5):505-513.
[11] Hema M,Joseph J,Gopinath K,et al.Molecular characterization and interviral relationships of a flexuous filamentous virus causing mosaic disease of sugarcane (Saccharum officinarum L.) in India[J].Archives of Virology,1999,144(3):479-490.
[12] Mayo MA.Changes to virus taxonomy[J].Archives of Virology,2005,150(1):189-198.
[13] Luo Q,Ahmad K,Fu HY,et al.Genetic diversity and population structure of Sorghum mosaic virus infecting Saccharum spp.hybrids[J].Ann Appl Biol,2016,169 (3):398-407.
[14] 李松,余坤兴,刘丽敏,等.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].江苏农业科学,2011,39(2):83-87.
[15] 王凯莉,邓权清,窦志敏,等.果蔗脱毒种苗甘蔗花叶病、黄叶病和宿根矮化病分子检测[J].植物保护,2018,44(3):163-167.
[16] Lockhart BEL,Autrey LJC.Occurrence in sugarcane of a bacilliform virus related serologically to banana streak virus[J].Plant Disease,1988,72(3):230-233.
[2] Autrey LJC,Boolell LJC,Jones S,et al.The distribution of sugarcane bacilliform virus in various geographical regions[J].Proc Int Soc Sugar Cane Technology,1995,21:527-541.
[3] Lockhart BEL,Autrey LJC.Sugarcane bacilliform virus[C]//Rott P,Bailey RA,Comstock JC,et al.A Guide to Sugarcane Diseases.Montpellier:CI-RAD-ISSCT Press,2000:268-272.
[4] Karuppaiah R,Viswanathan R,Kumar VG.Genetic diversity of sugarcane bacilliform virus isolates infecting Saccharum spp.in India[J].Virus Genes,2013,46(3):505-516.
[5] 蔡艳清,许东林,周国辉,等.广东首次发现甘蔗杆状病毒[C]//中国植物病理学会.中国植物病理学会2004年学术年会论文集.北京:中国农业科学技术出版社,2004:213-215.
[6] 徐正银,周龙武,唐瑶,等.广西甘蔗杆状病毒的分子检测及分布调查[J].南方农业学报,2015,46(11):1980-1984.
[7] 李文凤,黄应昆,姜冬梅,等.云南甘蔗杆状病毒的分子检测及其对甘蔗品质和产量的影响[J].植物病理学报,2010,40(6):651-654.
[8] 李瑞美,何炎森.不同类型果蔗品种资源氨基酸等营养成分分析[J].热带作物学报,2017,38(1):171-174.
[9] 余坤兴,谭芳,李松,等.广西果蔗生产现状与健康发展对策[J].广西蔗糖,2007(4):14-20.
[10] Seifer DL,Salomon R,Marie JV,et al.Characterization of a novel potyvirus isolated from maize in Israel[J].Phytopathology,2000,90(5):505-513.
[11] Hema M,Joseph J,Gopinath K,et al.Molecular characterization and interviral relationships of a flexuous filamentous virus causing mosaic disease of sugarcane (Saccharum officinarum L.) in India[J].Archives of Virology,1999,144(3):479-490.
[12] Mayo MA.Changes to virus taxonomy[J].Archives of Virology,2005,150(1):189-198.
[13] Luo Q,Ahmad K,Fu HY,et al.Genetic diversity and population structure of Sorghum mosaic virus infecting Saccharum spp.hybrids[J].Ann Appl Biol,2016,169 (3):398-407.
[14] 李松,余坤兴,刘丽敏,等.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].江苏农业科学,2011,39(2):83-87.
[15] 王凯莉,邓权清,窦志敏,等.果蔗脱毒种苗甘蔗花叶病、黄叶病和宿根矮化病分子检测[J].植物保护,2018,44(3):163-167.
[16] Lockhart BEL,Autrey LJC.Occurrence in sugarcane of a bacilliform virus related serologically to banana streak virus[J].Plant Disease,1988,72(3):230-233.