鄂西清风藤的化学成分研究
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摘要
采用75%乙醇对鄂西清风藤进行提取,利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和重结晶等方法对提取物进行分离纯化,并通过理化性质与波谱数据并结合文献对化合物结构进行鉴定,且从该植物中首次分离出6个化合物:齐墩果烷-9(11),12-二烯3-酮(1)、β-谷甾醇(2)、邻苯二甲酸异丁酯(3)、茴芹苦素(4)、3-oxo-11α-methoxyolean-12-ene(5)、3-氧-■-齐墩果二烯(6)。同时采用MTT法检测了化合物对Con A肝损伤细胞存活率的影响,结果显示化合物3,5和6,具有一定的抗肝损伤活性。
The branches of Sabia campanulata Wall. subsp. ritchieae were extracted with 75% ethanol,then separated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography,recrystallization,and so on,based on bioassay-guided method,while their structures were identified by physical and chemical properties and spectral data combined with literature. Six compounds were isolated and identified from this plant for the first time: Oleanane-9( 11),12-diene-3-one( 1),β-sitosterol( 2),butyl isobutylphthalate( 3),Pimpinellin( 4),3-oxo-11α-methoxyolean-12-ene( 5),3-oxo-■-oleanene( 6),and MTT results showed that compounds 3,5 and 6 exerted certain liver protection activity.
The branches of Sabia campanulata Wall. subsp. ritchieae were extracted with 75% ethanol,then separated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography,recrystallization,and so on,based on bioassay-guided method,while their structures were identified by physical and chemical properties and spectral data combined with literature. Six compounds were isolated and identified from this plant for the first time: Oleanane-9( 11),12-diene-3-one( 1),β-sitosterol( 2),butyl isobutylphthalate( 3),Pimpinellin( 4),3-oxo-11α-methoxyolean-12-ene( 5),3-oxo-■-oleanene( 6),and MTT results showed that compounds 3,5 and 6 exerted certain liver protection activity.
引文
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[2]陈谨,邓赟,唐天君,等.小花清风藤三萜成分的研究[J].中草药,2004,35(1):16-17.
[3] CHEN J,CHEN B,TIAN J,et al. A new benzylisoquinoline alkaloid from Sabia parviflora[J]. Chinese Chem Lett,2002,13(5):426-427.
[4] CHEN J,CHEN B,TIAN J,et al. Two new pentacyclic triterpenes from Sabia parviflora[J]. Chinese Chem Lett,2002,13(4):345-348.
[5]邓赟,陈谨,陈斌,等.小花清风藤生物碱成分的研究[J].天然产物研究与开发,2003,15(4):322-323.
[6]林佳,郝小江,梁光义,等.小花清风藤化学成分的研究[J].中草药,1999,30(5):334-335.
[7]林材,曹佩雪,梁光义.小花清风藤的化学成分研究[J].天然产物研究与开发,2006,18(6):42-44.
[8]陈艳,黄滔,苑春茂,等.小花清风藤化学成分的研究[J].中草药,2015,46(21):3146-3150.
[9]刘易蓉,邱晓春,陈惠.小花清风藤保肝作用实验研究[J].中国药房,2008,19(30):2341-2342.
[10]吴敬敬. JBP485对刀豆球蛋白A导致的原代培养大鼠肝细胞损伤的保护作用研究[D].大连:大连医科大学,2008:2-6.
[11] YUAN X,WANG G L,GONG F J. Studies on triterpenoid constituents isolated from the roots of Sabia schumanniana[J]. Acta Bot Sin,1994,36(2):153-158.
[12] LI W H,CHANG S T,CHANG S C,et al. Isolation of antibacterial diterpenoids from Cryptomeria japonica bark[J]. Nat Prod Res,2008,22(12):1085-1093.
[13] Bu T,Liu M,Zheng L,et al.α-Glucosidase inhibition and the in vivo hypoglycemic effect of butyl-isobutyl-phthalate derived from the Laminaria japonica rhizoid[J]. Phytotherapy Research Ptr,2010,24(11):1588-1591.
[14] YANG S J,LIU M C,LIANG N,et al. Discovery and antitumor activities of constituents from Cyrtomium fortumei(J.)Smith rhizomes[J]. Chemistry Central Journal,2013,7(1):24-34.
[15] ALMEIDAM T R D,LUCI C R,PADRON J M,et al. Antiproliferative terpenoids and alkaloids from the roots of Maytenus vitisidaea and Maytenus spinosa[J]. Phytochemistry,2010,71(14/15):1741-1748.