黄芩药材指纹图谱及其黄芩苷、黄芩素、汉黄芩素含量随地区分布差异的聚类分析
|
摘要
目的:建立20批南北地区黄芩药材的指纹图谱并测定其中有效成分黄芩苷、黄芩素、汉黄芩素的含量。方法:采用HPLC比较20批不同产地的黄芩药材的指纹图谱,并测定其中黄芩苷、黄芩素、汉黄芩素的含量。结果:20批药材中湖北十堰地区黄芩中黄芩苷含量最高,海南地区黄芩中黄芩素和汉黄芩素含量最高,且南北地区黄芩药材具有差异性。结论:该方法简便、专属性强、重现性好,可用于黄芩的质量控制。
Objective: To establish the fingerprint of 20 batches of Huangqin(Scutellaria baicalensis) and to determine the content of its active ingredient(Baicalin, Baicalein and Hanbaicalein) by HPLC. Methods: The fingerprint of 20 batches of Huangqin(Scutellaria baicalensis) were compared by HPLC. The Baicalin, Baicalein and Hanbaicalein were identified. Results: The content of baicalin produced by Shiyan is highest in 20 batches of Huangqin(Scutellaria baicalensis) and the content of Baicalein and Hanbaicalein produced by Hainan is highest in 20 batches of herbs; there`s obvious differences between the origin in south and north area. Conclusion: The method is simple, with strong specificity and good reproducibility, and it can be used in the quality control of the Huangqin(Scutellaria baicalensis).
Objective: To establish the fingerprint of 20 batches of Huangqin(Scutellaria baicalensis) and to determine the content of its active ingredient(Baicalin, Baicalein and Hanbaicalein) by HPLC. Methods: The fingerprint of 20 batches of Huangqin(Scutellaria baicalensis) were compared by HPLC. The Baicalin, Baicalein and Hanbaicalein were identified. Results: The content of baicalin produced by Shiyan is highest in 20 batches of Huangqin(Scutellaria baicalensis) and the content of Baicalein and Hanbaicalein produced by Hainan is highest in 20 batches of herbs; there`s obvious differences between the origin in south and north area. Conclusion: The method is simple, with strong specificity and good reproducibility, and it can be used in the quality control of the Huangqin(Scutellaria baicalensis).
引文
[1]国家药典委员会.中国药典(一部)[M].北京:化学工业出版社,2015:301-302.
[2]杨巧芳,孟庆刚,洪志强,等.黄芩泻火解毒功效评述[J].中华中医药学刊,2007,25(8):1597.
[3]肖培根.新编中药志(第1卷)[M].北京:化学工业出版社,2002:862.
[4] Li H B,Jiang Y,Chen F. Separation methods used for Scutellaria baicalensis active components[J].Chromatogr B Analyt Technol Biomed Life Sci,2004(812):277-290.
[5]施高翔,邵菁,汪天明,等.黄芩及其有效成分抗菌作用新进展[J].中国中药杂志,2014,39(19):3713-3718.
[6] Wang H,Yu C,Gao J,et al. Effects of processing and extractingmethods on active components in Radix Scutellariae by HPLC analysis[J].China journal of Chinese materia medica,2007(32):1637.
[7]肖蓉,袁志芳,王春英,等.不同产地黄芩药材HPLC指纹图谱的研究[J].中草药,2005,36(5):743-747.
[8]薛黎明,秦雪梅,张丽增.不同产地黄芩药材的黄芩苷含量测定及指纹图谱研究[J].中成药,2008,30(1):10-13.
[9]宋双红,王喆之,张媛,等.黄芩药材HPLC指纹图谱的研究[J].中国药学杂志,2006,41(6):413-417.
[10] Technical Requirement of the Fin gerp rintin Injection of Chinese Mater ia Medica(Tentative Stand ard)[中药注射剂指纹图谱研究的技术要求(暂行)][S].2001.
[11] Xiao R,Zhang L T. Actuality and future of studies on finger-prints of TCM[J].J Hebei Med Univ,2003,24(5):310-313.
[2]杨巧芳,孟庆刚,洪志强,等.黄芩泻火解毒功效评述[J].中华中医药学刊,2007,25(8):1597.
[3]肖培根.新编中药志(第1卷)[M].北京:化学工业出版社,2002:862.
[4] Li H B,Jiang Y,Chen F. Separation methods used for Scutellaria baicalensis active components[J].Chromatogr B Analyt Technol Biomed Life Sci,2004(812):277-290.
[5]施高翔,邵菁,汪天明,等.黄芩及其有效成分抗菌作用新进展[J].中国中药杂志,2014,39(19):3713-3718.
[6] Wang H,Yu C,Gao J,et al. Effects of processing and extractingmethods on active components in Radix Scutellariae by HPLC analysis[J].China journal of Chinese materia medica,2007(32):1637.
[7]肖蓉,袁志芳,王春英,等.不同产地黄芩药材HPLC指纹图谱的研究[J].中草药,2005,36(5):743-747.
[8]薛黎明,秦雪梅,张丽增.不同产地黄芩药材的黄芩苷含量测定及指纹图谱研究[J].中成药,2008,30(1):10-13.
[9]宋双红,王喆之,张媛,等.黄芩药材HPLC指纹图谱的研究[J].中国药学杂志,2006,41(6):413-417.
[10] Technical Requirement of the Fin gerp rintin Injection of Chinese Mater ia Medica(Tentative Stand ard)[中药注射剂指纹图谱研究的技术要求(暂行)][S].2001.
[11] Xiao R,Zhang L T. Actuality and future of studies on finger-prints of TCM[J].J Hebei Med Univ,2003,24(5):310-313.