弓形虫感染患儿淋巴细胞亚群及干扰素-γ的变化及临床意义
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摘要
目的探讨淋巴细胞各亚群及细胞免疫应答产物干扰素-γ(IFN-γ)水平在弓形虫感染患儿细胞免疫应答中的变化趋势及临床意义。方法选取2011年1月至2014年4月检测出Tox-Ig M阳性的34例患儿为Tox-Ig M阳性组,同时选取既往感染的TOX-Ig G阳性患儿12例为TOX-Ig G阳性组,另选取行健康体检的健康儿童54例为对照组。应用流式细胞仪检测各组外周血淋巴细胞CD4+、CD8+、CD19+和NK细胞水平,并计算CD4+/CD8+比值;采用酶联免疫吸附试验(ELISA)检测各组患儿血清中IFN-γ的水平。结果 Tox-Ig M阳性组患儿CD4+、CD4+/CD8+比值明显低于TOX-Ig G阳性组和对照组,CD8+、NK细胞和血清IFN-γ水平高于TOXIg G阳性组和对照组(P<0.05)。TOX-Ig G阳性组患儿血清IFN-γ水平高于对照组(P<0.05)。Tox-Ig M阳性组患儿CD8+水平与血清IFN-γ水平呈正相关(r=0.756,P<0.05)。结论 CD4+、CD8+以及NK细胞等免疫细胞及其免疫应答产物IFN-γ水平变化在弓形虫感染过程中发挥着重要的作用。
Objective To study the changes and significance of lymphocyte sunsets and serum interferon-γ(IFN-γ) levels in children with toxoplasma infection. Methods Thirty-four children who were newly diagnosed with toxoplasma infection(TOX-Ig M+ group) between January 2011 and April 2014, 12 children who had ever been diagnosed with toxoplasma infection(TOX-Ig G+ group), and 54 healthy children(control group) were enrolled. The percentages of CD4+, CD8+, CD19+ and NK cells in peripheral blood lymphocytes were detected by flow cytometry. Serum levels of IFN-γ were measured using ELISA. Results The percentages of CD4+ cells and the CD4+/CD8+ ratio in the TOX-Ig M+ group were significantly lower than in the TOX-Ig G+ and control groups, while the percentages of CD8+ and NK cells and serum IFN-γ levels were significantly higher than in the other two groups(P<0.05). The TOXIg G+ group had higher serum IFN-γ levels than the control group(P<0.05). There was a positive correlation between the percentage of CD8+ cells and serum IFN-γ levels in the TOX-Ig M+ group(r=0.756; P<0.05). Conclusions CD4+, CD8+ and NK cells may play important roles in the resistance against toxoplasma infection by promoting the secretion of cytokines.
Objective To study the changes and significance of lymphocyte sunsets and serum interferon-γ(IFN-γ) levels in children with toxoplasma infection. Methods Thirty-four children who were newly diagnosed with toxoplasma infection(TOX-Ig M+ group) between January 2011 and April 2014, 12 children who had ever been diagnosed with toxoplasma infection(TOX-Ig G+ group), and 54 healthy children(control group) were enrolled. The percentages of CD4+, CD8+, CD19+ and NK cells in peripheral blood lymphocytes were detected by flow cytometry. Serum levels of IFN-γ were measured using ELISA. Results The percentages of CD4+ cells and the CD4+/CD8+ ratio in the TOX-Ig M+ group were significantly lower than in the TOX-Ig G+ and control groups, while the percentages of CD8+ and NK cells and serum IFN-γ levels were significantly higher than in the other two groups(P<0.05). The TOXIg G+ group had higher serum IFN-γ levels than the control group(P<0.05). There was a positive correlation between the percentage of CD8+ cells and serum IFN-γ levels in the TOX-Ig M+ group(r=0.756; P<0.05). Conclusions CD4+, CD8+ and NK cells may play important roles in the resistance against toxoplasma infection by promoting the secretion of cytokines.
引文
[1]姚天一,张志坤.弓形虫感染与围生儿预后[J].中国实用妇科和产科杂志,2005,2(6):339-341.
[2]廖洪,郭奕明,徐龙,等.807例住院儿童弓形虫感染调查分析[J].中国当代儿科杂志,2002,4(4):291-293.
[3]彭鸿娟.刚地弓形虫纳虫泡的形成机制及其作用[J].中国寄生虫学及寄生虫病杂志,2010,28(5):382-386.
[4]Jankovic D,Kugler DG,Sher A,et al.IL-10 production by CD4+effector T cell:a mechanism for self regulation[J].Mucosal Immunol,2010,3(3):239-246.
[5]杨生海,殷宏,刘永生,等.干扰素-γ研究进展[J].生物技术通报,2010,8(1):29-33.
[6]蒋灿华.NK细胞抗肿瘤免疫效应机制研究进展[J].国外医学肿瘤学分册,2004,31(7):486-489.
[7]李永念,包怀恩.BALB/c小鼠感染弓形虫后Th1/Th2免疫漂移的实验研究[J].中国免疫学杂志,2009,3(25):209-212.
[8]Kim WH,Shin EH,Kim JL,et al.Suppression of CD4+T-cells in the spleen of mice infection with toxoplasma gondii KI-I tachyzoites[J].Korean J Parasitol,2010,48(4):325-329.
[9]Mandell GL,Bennett JE,Dolin R.Toxoplasma gondii[M]//Desmonts G.Principles and practice of infectious diseases.5th ed.Beijing:Science Press,2001:2687.
[10]Cui W,Kaech SM.Generation of effector CD8+T cells and their conversion to memory T cells[J].Immunol Rev,2010,236:151-166.
[11]Gigley JP,Bhadra R,Khan IA.CD8+T cells and toxoplasma gondii:a new paradigm[J].J Parasitol Res,2011,2011:243796.
[12]高冬梅,赵俊,夏圆圆,等.弓形虫先天感染与妊娠结局及脐带血T淋巴细胞亚群构成的关系[J].中华预防医学杂志,2012,46(1):64-66.
[13]Werry EJ.T cell exhaustion[J].Nat Immunol,2011,12(6):492-499.
[14]李越,陈伟,范雄林,等.Granulysin的研究进展[J].细胞与分子免疫学杂志,2002,18(5):108-109.
[15]Miller CM,Boulter NR,Ikin RJ,et al.The immunobiology of the innate response to toxoplasma gondii[J].Int J Parasitol,2009,39(1):23-39.
[2]廖洪,郭奕明,徐龙,等.807例住院儿童弓形虫感染调查分析[J].中国当代儿科杂志,2002,4(4):291-293.
[3]彭鸿娟.刚地弓形虫纳虫泡的形成机制及其作用[J].中国寄生虫学及寄生虫病杂志,2010,28(5):382-386.
[4]Jankovic D,Kugler DG,Sher A,et al.IL-10 production by CD4+effector T cell:a mechanism for self regulation[J].Mucosal Immunol,2010,3(3):239-246.
[5]杨生海,殷宏,刘永生,等.干扰素-γ研究进展[J].生物技术通报,2010,8(1):29-33.
[6]蒋灿华.NK细胞抗肿瘤免疫效应机制研究进展[J].国外医学肿瘤学分册,2004,31(7):486-489.
[7]李永念,包怀恩.BALB/c小鼠感染弓形虫后Th1/Th2免疫漂移的实验研究[J].中国免疫学杂志,2009,3(25):209-212.
[8]Kim WH,Shin EH,Kim JL,et al.Suppression of CD4+T-cells in the spleen of mice infection with toxoplasma gondii KI-I tachyzoites[J].Korean J Parasitol,2010,48(4):325-329.
[9]Mandell GL,Bennett JE,Dolin R.Toxoplasma gondii[M]//Desmonts G.Principles and practice of infectious diseases.5th ed.Beijing:Science Press,2001:2687.
[10]Cui W,Kaech SM.Generation of effector CD8+T cells and their conversion to memory T cells[J].Immunol Rev,2010,236:151-166.
[11]Gigley JP,Bhadra R,Khan IA.CD8+T cells and toxoplasma gondii:a new paradigm[J].J Parasitol Res,2011,2011:243796.
[12]高冬梅,赵俊,夏圆圆,等.弓形虫先天感染与妊娠结局及脐带血T淋巴细胞亚群构成的关系[J].中华预防医学杂志,2012,46(1):64-66.
[13]Werry EJ.T cell exhaustion[J].Nat Immunol,2011,12(6):492-499.
[14]李越,陈伟,范雄林,等.Granulysin的研究进展[J].细胞与分子免疫学杂志,2002,18(5):108-109.
[15]Miller CM,Boulter NR,Ikin RJ,et al.The immunobiology of the innate response to toxoplasma gondii[J].Int J Parasitol,2009,39(1):23-39.