运动结合中药复方对去势大鼠股骨生物力学影响的研究
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摘要
目的:探讨运动结合中药复方对去势大鼠骨生物力学改善的作用。方法:取180日龄SPF级SD雌性大鼠100只,随机分为空白组、模型组、运动组、中药复方组、运动结合中药复方组,每组20只,造模术后常规饲养14天。之后除空白组外,各组分别灌胃治疗,模型组每天给予0.9%氯化钠注射液2ml灌胃;运动组每天给予0.9%氯化钠注射液2ml灌胃,并在高速平板跑台跑步20min,速度为5m/min;中药复方组每天给予中药复方溶液2ml灌胃;运动结合中药复方组每天给予中药复方溶液2ml灌胃,并在高速平板跑台跑步20min,速度为5m/min。干预治疗后12周后将大鼠戊巴比妥腹腔注射麻醉,处死,收集双侧股骨,剔净附着于股骨上的肌肉及结缔组织,分别进行三点弯曲试验、显微结构观察及骨小梁静态参数检测。结果:与模型组比较,空白组、运动组、中药复方组、运动结合中药复方组在骨密度、股骨载荷、骨小梁静态参数检测指标均有明显差异(P<0.01或P<0.05)。结论:运动结合中药复方具有改善去势大鼠骨生物力学指标,保护骨小梁结构的作用。
Objective: To explore the rats bone biomechanics improvement action after exercise combined with traditional Chinese medicine compound of castration.Method: A total of 100 SPF SD female rats, aged 180 days, were randomly divided into blank group,model group,exercise,traditional Chinese medicine compound,sports combined with traditional Chinese medicine compound group, 20 in each group. Followed conventional breeding 14 days after surgery. 2 ml 0.9% sodium chloride was filled to stomach daily for model group and exercise group. 2 ml traditional Chinese medicine compound solution was filled to the stomach daily for the traditional Chinese medicine compound and sports combined with traditional Chinese medicine compound group. Besides, the rats were forced to running on flat treadmill 20 minutes daily at speed of 5 m/min for exercise group and sports combined with traditional Chinese medicine compound group. After 12 weeks treatment, bilateral femur were collected, attached muscle and connective tissue were took out. Then three-point bending test, microstructure observation, and static parameters of bone trabecula test were completed.Result: BMD, maximal load of femur, and static parameters of bone trabecula test for model group, blank group, exercise group, Chinese medicine compound group, exercise combined with traditional Chinese medicine compound group all have obvious difference from the model group(P<0.01 or P<0.05).Conclusion: Exercise combined with traditional Chinese medicine compound is beneficial for treatment of osteoporosis in castrated rats.
Objective: To explore the rats bone biomechanics improvement action after exercise combined with traditional Chinese medicine compound of castration.Method: A total of 100 SPF SD female rats, aged 180 days, were randomly divided into blank group,model group,exercise,traditional Chinese medicine compound,sports combined with traditional Chinese medicine compound group, 20 in each group. Followed conventional breeding 14 days after surgery. 2 ml 0.9% sodium chloride was filled to stomach daily for model group and exercise group. 2 ml traditional Chinese medicine compound solution was filled to the stomach daily for the traditional Chinese medicine compound and sports combined with traditional Chinese medicine compound group. Besides, the rats were forced to running on flat treadmill 20 minutes daily at speed of 5 m/min for exercise group and sports combined with traditional Chinese medicine compound group. After 12 weeks treatment, bilateral femur were collected, attached muscle and connective tissue were took out. Then three-point bending test, microstructure observation, and static parameters of bone trabecula test were completed.Result: BMD, maximal load of femur, and static parameters of bone trabecula test for model group, blank group, exercise group, Chinese medicine compound group, exercise combined with traditional Chinese medicine compound group all have obvious difference from the model group(P<0.01 or P<0.05).Conclusion: Exercise combined with traditional Chinese medicine compound is beneficial for treatment of osteoporosis in castrated rats.
引文
[1]韦大文,尚立芝,李沛,等.补肾方治疗去势雌鼠骨质疏松及其机制的研究[J].中国实验方剂学杂志,2010,16(10):125-127.
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[10]姜俊良,梁邱,杨浩伦,等.低频脉冲电磁场治疗绝经后骨质疏松症的临床疗效观察[J].中国康复医学,2017,32(2):192-194.
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[12]黄何平.原发性骨质疏松症及其运动处方[J].中国临床康复,2006,10(32):132-134.
[13]陈亚琼,杨海燕,黄艳红,等.补肾中药醇提活性部位预防卵巢摘除小鼠的骨丢失[J].中国骨质疏松杂志,2002,8(1):68-72.
[14]林勇凯,黄宇新.补肾调肝方治疗高龄骨质疏松症伴抑郁障碍32例疗效观察[J].中医杂志,2014,55(2):137-139.
[15]刘旦锋,崔碧玲,黄银玲,等.辅酶Q10对环磷酰胺大鼠皮质骨的影响[J].中国药学杂志,2016,51(2):110-114.
[16]陈国仙,王国荣,李国山,等.低强度复合振动联合阿仑膦酸钠对绝经后骨质疏松患者骨密度的影响研究[J].中国康复医学杂志,2017,32(2):182-186.
[17]陈玉凤,熊鹰,陆一帆,等.运动对绝经后骨折女性ALP、BMD、E2的影响[J].天津体育学院学报,2004,19(1):94-95.
[18]王伯华.运动康复处方联合补肾活血方对急性期髋部骨折髋关节功能、骨生物力学和骨密度的影响[J].中国中医急症,2016,25(4):694-696.
[19]宰衷静.固邦联合金乌骨通胶囊治疗骨质疏松性腰背疼痛[J].中医正骨,2009,21(5):53-54.
[20]闵文,黄桂成,华永庆,等.补肾通络方治疗去势大鼠骨质疏松的研究[J].中国实验方剂学杂志,2010,16(17):189-192.
[21]赵冰洁,宋捷,章丽,等.淫羊藿醇提物对去卵巢大鼠骨质疏松治疗作用及机制研究[J].中草药,2016,47(11):1919-1925.
[2]龙梓,刘丹霞,廖华卫,等.健骨片对去卵巢骨质疏松大鼠骨代谢、骨微结构及骨强度的影响[J].中国实验方剂学杂志,2016,22(9):116-121.
[3]Rossouw JE,Anderson GL,Prentice RL,et al.Risks and benefits of estrogen plus progestin in healthy postmenopausa women:principal results From the Women's Health Initiative randomized controlled trial[J].JAMA,2002,288(3):321-333.
[4]国外研究快讯.保护骨骼健康预防因骨质疏松和低骨密度而增高骨折危险[J].中国矫形外科杂志,2005,13(8):604.
[5]白云静,申洪波,张秋枫,等.原发性骨质疏松症从肾论治的理论探讨[J].中国矫形外科杂志,2002,9(6):619-620.
[6]Downey PA,Siegel MI.Bone biology and the clinical implications for osteoporosis[J].Physical Therapy,2006,1:77-92.
[7]Elaine MM.Designing exercise programs to lower fracture risk in mature women[J].Strength and Conditioning Journal,2006,1:24-30.
[8]楼华.老年性骨质疏松及其运动疗法的干预[J].中国组织工程研究与临床康复,2007,52:10672-10673.
[9]孙建翠,崔西泉.有氧健身运动对老年性骨质疏松的影响[J].中国矫形外科杂志,2009,17(17):1326-1328.
[10]姜俊良,梁邱,杨浩伦,等.低频脉冲电磁场治疗绝经后骨质疏松症的临床疗效观察[J].中国康复医学,2017,32(2):192-194.
[11]邹军,章岚,任弘,等.运动防治骨质疏松专家共识[J].中国骨质疏松杂志,2015,21(11):1291-1302.
[12]黄何平.原发性骨质疏松症及其运动处方[J].中国临床康复,2006,10(32):132-134.
[13]陈亚琼,杨海燕,黄艳红,等.补肾中药醇提活性部位预防卵巢摘除小鼠的骨丢失[J].中国骨质疏松杂志,2002,8(1):68-72.
[14]林勇凯,黄宇新.补肾调肝方治疗高龄骨质疏松症伴抑郁障碍32例疗效观察[J].中医杂志,2014,55(2):137-139.
[15]刘旦锋,崔碧玲,黄银玲,等.辅酶Q10对环磷酰胺大鼠皮质骨的影响[J].中国药学杂志,2016,51(2):110-114.
[16]陈国仙,王国荣,李国山,等.低强度复合振动联合阿仑膦酸钠对绝经后骨质疏松患者骨密度的影响研究[J].中国康复医学杂志,2017,32(2):182-186.
[17]陈玉凤,熊鹰,陆一帆,等.运动对绝经后骨折女性ALP、BMD、E2的影响[J].天津体育学院学报,2004,19(1):94-95.
[18]王伯华.运动康复处方联合补肾活血方对急性期髋部骨折髋关节功能、骨生物力学和骨密度的影响[J].中国中医急症,2016,25(4):694-696.
[19]宰衷静.固邦联合金乌骨通胶囊治疗骨质疏松性腰背疼痛[J].中医正骨,2009,21(5):53-54.
[20]闵文,黄桂成,华永庆,等.补肾通络方治疗去势大鼠骨质疏松的研究[J].中国实验方剂学杂志,2010,16(17):189-192.
[21]赵冰洁,宋捷,章丽,等.淫羊藿醇提物对去卵巢大鼠骨质疏松治疗作用及机制研究[J].中草药,2016,47(11):1919-1925.