两步预处理对厌氧消化产酸纤维酶解和沼气发酵的影响
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摘要
对厌氧消化产酸纤维(挥发性脂肪酸生产废渣)进行了酶解糖化和沼气发酵实验研究,探索了乙酸氨水两步预处理对木质纤维素降解、葡萄糖浓度、木糖浓度和沼气产率的影响。结果表明:乙酸氨水两步预处理进一步降解了厌氧消化产酸中的半纤维素和木质素,而且纤维素回收率较高,为77.47%~81.44%,其中4%乙酸处理和10%氨水处理实验组的纤维素质量分数达到56.82%,半纤维素质量分数仅为7.94%;在上述预处理条件下,酶解糖化效率最高,葡萄糖质量浓度达到47.46 g/L,葡萄糖得率为67.73%;同时在此乙酸氨水两步预处理条件下的沼气产率明显优于单独乙酸和单独氨水预处理,获得的最高沼气产率为414 mL/g,有效提高了厌氧消化产酸纤维的产气效率。
The enzymatic hydrolysis and biogas fermentation of anaerobically digested fiber from volatile fatty acids( VFAs) production with rice straw were studied. The effects of two-step pretreatment of acetic acid and aqueous ammonia on the degradation of lignocellulose, glucose concentration, xylose concentration and biogas production were explored. The results showed that two-step pretreatment of acetic acid and aqueous ammonia can further degrade the hemicellulose and lignin in anaerobically digested fiber from VFAs production,and the recovery rate of cellulose was higher,which was 77. 47% ~81. 44%. Among them, the mass fraction of cellulose was 56. 82% and the mass fraction of hemicellulose was only 7. 94% for pretreatment of 4% acetic acid and 10% aqueous ammonia at 121℃.Under the above pretreatment conditions,the enzymatic saccharification efficiency was the highest,the mass concentration of glucose was 47. 46 g/L,and the glucose yield was 67. 73%. Furthermore,the biogas production rate of anaerobically digested fiber from volatile fatty acids production pretreated by twostep pretreatment was better than those of acetic acid pretreatment alone and aqueous ammonia pretreatment alone. The highest biogas yield( 414 m L/g) was obtained. Two-step pretreatment effectively improved the biogas yield of anaerobically digested fiber from VFAs production. Therefore,the research proved a new biorefinery way of anaerobically digested fiber.
The enzymatic hydrolysis and biogas fermentation of anaerobically digested fiber from volatile fatty acids( VFAs) production with rice straw were studied. The effects of two-step pretreatment of acetic acid and aqueous ammonia on the degradation of lignocellulose, glucose concentration, xylose concentration and biogas production were explored. The results showed that two-step pretreatment of acetic acid and aqueous ammonia can further degrade the hemicellulose and lignin in anaerobically digested fiber from VFAs production,and the recovery rate of cellulose was higher,which was 77. 47% ~81. 44%. Among them, the mass fraction of cellulose was 56. 82% and the mass fraction of hemicellulose was only 7. 94% for pretreatment of 4% acetic acid and 10% aqueous ammonia at 121℃.Under the above pretreatment conditions,the enzymatic saccharification efficiency was the highest,the mass concentration of glucose was 47. 46 g/L,and the glucose yield was 67. 73%. Furthermore,the biogas production rate of anaerobically digested fiber from volatile fatty acids production pretreated by twostep pretreatment was better than those of acetic acid pretreatment alone and aqueous ammonia pretreatment alone. The highest biogas yield( 414 m L/g) was obtained. Two-step pretreatment effectively improved the biogas yield of anaerobically digested fiber from VFAs production. Therefore,the research proved a new biorefinery way of anaerobically digested fiber.
引文
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4 ARAMRUEANG N,ZICARI S M,ZHANG R.Characterization and compositional analysis of agricultural crops and residues for ethanol production in California[J].Biomass and Bioenergy,2017,105:288-297.
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7 WANG D,XI J,AI P,et al.Enhancing ethanol production from thermophilic and mesophilic solid digestate using ozone combined with aqueous ammonia pretreatment[J].Bioresource Technology,2016,207:52-58.
8 MACLELLAN J,CHEN R,KRAEMER R,et al.Anaerobic treatment of lignocellulosic material to co-produce methane and digested fiber for ethanol biorefining[J].Bioresource Technology,2013,130:418-423.
9 王殿龙,艾平,鄢烈亮,等.稻秸厌氧消化纤维制取乙醇实验研究[J/OL].农业机械学报,2015,46(5):156-163.http:∥www.j-csam.org/jcsam/ch/reader/view_abstract.aspx?flag=1&file_no=20150521&journal_id=jcsam.DOI:10.6041/j.issn.1000-1298.2015.05.021.WANG Dianlong,AI Ping,YAN Lieliang,et al.Research on ethanol production of anaerobic digestion fiber from rice straw[J/OL].Transactions of the Chinese Society for Agricultural Machinery,2015,46(5):156-163.(in Chinese)
10 VEGI S,SHASTRI Y.Optimal control of dilute acid pretreatment and enzymatic hydrolysis for processing lignocellulosic feedstock[J].Journal of Process Control,2017,56:100-111.
11 王健,赵玲,田萌萌,等.组合碱预处理对玉米秸秆厌氧消化的影响[J].太阳能学报,2014,35(12):2577-2581.WANG Jian,ZHAO Ling,TIAN Mengmeng,et al.Influence of combined alkali pretreatment on anaerobic digestion of corn stalk[J].Acta Energiae Solaris Sinica,2014,35(12):2577-2581.(in Chinese)
12 张万钦,戚丹丹,吴树彪,等.不同预处理方式对污泥厌氧发酵的影响[J/OL].农业机械学报,2014,45(9):187-198 .http:∥www.j-csam.org/jcsam/ch/reader/view_abstract.aspx?flag=1&file_no=20140931&journal_id=jcsam.DOI:10.6041/j.issn.1000-1298.2014.09.031.ZHANG Wanqin,QI Dandan,WU Shubiao,et al.Effect of different pretreatment methods on sewage sludge anaerobic digestion[J/OL].Transactions of the Chinese Society for Agricultural Machinery,2014,45(9):187-198.(in Chinese)
13 GAO A H,BULE M V,LASKAR D D,et al.Structural and thermal characterization of wheat straw pretreated with aqueous ammonia soaking[J].Journal of Agricultural and Food Chemistry,2012,60(35):8632-8639.
14 MATHEW A K,PARAMESHWARAN B,SUKUMARAN R K,et al.An evaluation of dilute acid and ammonia fiber explosion pretreatment for cellulosic ethanol production[J].Bioresource Technology,2016,199:13-20.
15 CHANG H N,KIM N J,KANG J,et al.Biomass-derived volatile fatty acid platform for fuels and chemicals[J].Biotechnology and Bioprocess Engineering,2010,15(1):1-10.
16 YU Q,ZHUANG X,WANG W,et al.Hemicellulose and lignin removal to improve the enzymatic digestibility and ethanol production[J].Biomass and Bioenergy,2016,94:105-109.
17 HE Y,PANG Y,LIU Y,et al.Physicochemical characterization of rice straw pretreated with sodium hydroxide in the solid state for enhancing biogas production[J].Energy&Fuels,2008,22(4):2775-2781.
18 刘战广,朱洪光,王彪,等.粪草比对干式厌氧发酵产沼气效果的影响[J].农业工程学报,2009,25(4):196-200.LIU Zhanguang,ZHU Hongguang,WANG Biao,et al.Effect of ratios of manure to crop on dry anaerobic digestion for biogas production[J].Transactions of the CASE,2009,25(4):196-200.(in Chinese)
19 WANG D,AI P,YU L,et al.Comparing the hydrolysis and biogas production performance of alkali and acid pretreatments of rice straw using two-stage anaerobic fermentation[J].Biosystems Engineering,2015,132:47-55.
20 覃国栋,刘荣厚,孙辰.酸预处理对水稻秸秆沼气发酵的影响[J].上海交通大学学报:农业科学版,2011,29(1):58-61.QIN Guodong,LIU Ronghou,SUN Chen.Effects of acid pretreatment on biogas fermentation of rice straw[J].Journal of Shanghai Jiaotong University:Agricultural Science Edition,2011,29(1):58-61.(in Chinese)
21 钱玉婷,杜静,陈广银,等.温和水热预处理促进秸秆产沼气的条件优化研究[J].中国环境科学,2016,36(12):3703-3710.QIAN Yuting,DU Jing,CHEN Guangyin,et al.Optimization of conditions for promoting biogas production with hydrothermal pretreatment for straw[J].China Environment Science,2016,36(12):3703-3710.(in Chinese)
22 AMNUAYCHEEWA P,HENGAROONPRASAN R,RATTANAPORN K,et al.Enhancing enzymatic hydrolysis and biogas production from rice straw by pretreatment with organic acids[J].Industrial Crops and Products,2016,87:247-254.
23 宋安东,任天宝,张百良.玉米秸秆生产燃料乙醇的经济性分析[J].农业工程学报,2010,26(6):283-286.SONG Andong,REN Tianbao,ZHANG Bailiang.Economic analysis for production of fuel ethanol with corn straw[J].Transactions of the CASE,2010,26(6):283-286.(in Chinese)
24 YUE Z,TEATER C,MACLELLAN J,et al.Development of a new bioethanol feedstock-anaerobically digested fiber from confined dairy operations using different digestion configurations[J].Biomass and Bioenergy,2011,35(5):1946-1953.
2 吴创之,周肇秋,阴秀丽,等.我国生物质能源发展现状与思考[J].农业机械学报,2009,40(1):91-99.WU Chuangzhi,ZHOU Zhaoqiu,YIN Xiuli,et al.Current status of biomass energy development in China[J].Transactions of the Chinese Society for Agricultural Machinery,2009,40(1):91-99.(in Chinese)
3 ABDESHAHIAN P,LIM J S,HO W S,et al.Potential of biogas production from farm animal waste in Malaysia[J].Renewable and Sustainable Energy Reviews,2016,60:714-723.
4 ARAMRUEANG N,ZICARI S M,ZHANG R.Characterization and compositional analysis of agricultural crops and residues for ethanol production in California[J].Biomass and Bioenergy,2017,105:288-297.
5 CHEN Y,JIANG X,XIAO K,et al.Enhanced volatile fatty acids(VFAs)production in a thermophilic fermenter with stepwise p H increase-investigation on dissolved organic matter transformation and microbial community shift[J].Water Research,2017,112:261-268.
6 KIM N J,PARK G W,KANG J,et al.Volatile fatty acid production from lignocellulosic biomass by lime pretreatment and its applications to industrial biotechnology[J].Biotechnology and Bioprocess Engineering,2013,18(6):1163-1168.
7 WANG D,XI J,AI P,et al.Enhancing ethanol production from thermophilic and mesophilic solid digestate using ozone combined with aqueous ammonia pretreatment[J].Bioresource Technology,2016,207:52-58.
8 MACLELLAN J,CHEN R,KRAEMER R,et al.Anaerobic treatment of lignocellulosic material to co-produce methane and digested fiber for ethanol biorefining[J].Bioresource Technology,2013,130:418-423.
9 王殿龙,艾平,鄢烈亮,等.稻秸厌氧消化纤维制取乙醇实验研究[J/OL].农业机械学报,2015,46(5):156-163.http:∥www.j-csam.org/jcsam/ch/reader/view_abstract.aspx?flag=1&file_no=20150521&journal_id=jcsam.DOI:10.6041/j.issn.1000-1298.2015.05.021.WANG Dianlong,AI Ping,YAN Lieliang,et al.Research on ethanol production of anaerobic digestion fiber from rice straw[J/OL].Transactions of the Chinese Society for Agricultural Machinery,2015,46(5):156-163.(in Chinese)
10 VEGI S,SHASTRI Y.Optimal control of dilute acid pretreatment and enzymatic hydrolysis for processing lignocellulosic feedstock[J].Journal of Process Control,2017,56:100-111.
11 王健,赵玲,田萌萌,等.组合碱预处理对玉米秸秆厌氧消化的影响[J].太阳能学报,2014,35(12):2577-2581.WANG Jian,ZHAO Ling,TIAN Mengmeng,et al.Influence of combined alkali pretreatment on anaerobic digestion of corn stalk[J].Acta Energiae Solaris Sinica,2014,35(12):2577-2581.(in Chinese)
12 张万钦,戚丹丹,吴树彪,等.不同预处理方式对污泥厌氧发酵的影响[J/OL].农业机械学报,2014,45(9):187-198 .http:∥www.j-csam.org/jcsam/ch/reader/view_abstract.aspx?flag=1&file_no=20140931&journal_id=jcsam.DOI:10.6041/j.issn.1000-1298.2014.09.031.ZHANG Wanqin,QI Dandan,WU Shubiao,et al.Effect of different pretreatment methods on sewage sludge anaerobic digestion[J/OL].Transactions of the Chinese Society for Agricultural Machinery,2014,45(9):187-198.(in Chinese)
13 GAO A H,BULE M V,LASKAR D D,et al.Structural and thermal characterization of wheat straw pretreated with aqueous ammonia soaking[J].Journal of Agricultural and Food Chemistry,2012,60(35):8632-8639.
14 MATHEW A K,PARAMESHWARAN B,SUKUMARAN R K,et al.An evaluation of dilute acid and ammonia fiber explosion pretreatment for cellulosic ethanol production[J].Bioresource Technology,2016,199:13-20.
15 CHANG H N,KIM N J,KANG J,et al.Biomass-derived volatile fatty acid platform for fuels and chemicals[J].Biotechnology and Bioprocess Engineering,2010,15(1):1-10.
16 YU Q,ZHUANG X,WANG W,et al.Hemicellulose and lignin removal to improve the enzymatic digestibility and ethanol production[J].Biomass and Bioenergy,2016,94:105-109.
17 HE Y,PANG Y,LIU Y,et al.Physicochemical characterization of rice straw pretreated with sodium hydroxide in the solid state for enhancing biogas production[J].Energy&Fuels,2008,22(4):2775-2781.
18 刘战广,朱洪光,王彪,等.粪草比对干式厌氧发酵产沼气效果的影响[J].农业工程学报,2009,25(4):196-200.LIU Zhanguang,ZHU Hongguang,WANG Biao,et al.Effect of ratios of manure to crop on dry anaerobic digestion for biogas production[J].Transactions of the CASE,2009,25(4):196-200.(in Chinese)
19 WANG D,AI P,YU L,et al.Comparing the hydrolysis and biogas production performance of alkali and acid pretreatments of rice straw using two-stage anaerobic fermentation[J].Biosystems Engineering,2015,132:47-55.
20 覃国栋,刘荣厚,孙辰.酸预处理对水稻秸秆沼气发酵的影响[J].上海交通大学学报:农业科学版,2011,29(1):58-61.QIN Guodong,LIU Ronghou,SUN Chen.Effects of acid pretreatment on biogas fermentation of rice straw[J].Journal of Shanghai Jiaotong University:Agricultural Science Edition,2011,29(1):58-61.(in Chinese)
21 钱玉婷,杜静,陈广银,等.温和水热预处理促进秸秆产沼气的条件优化研究[J].中国环境科学,2016,36(12):3703-3710.QIAN Yuting,DU Jing,CHEN Guangyin,et al.Optimization of conditions for promoting biogas production with hydrothermal pretreatment for straw[J].China Environment Science,2016,36(12):3703-3710.(in Chinese)
22 AMNUAYCHEEWA P,HENGAROONPRASAN R,RATTANAPORN K,et al.Enhancing enzymatic hydrolysis and biogas production from rice straw by pretreatment with organic acids[J].Industrial Crops and Products,2016,87:247-254.
23 宋安东,任天宝,张百良.玉米秸秆生产燃料乙醇的经济性分析[J].农业工程学报,2010,26(6):283-286.SONG Andong,REN Tianbao,ZHANG Bailiang.Economic analysis for production of fuel ethanol with corn straw[J].Transactions of the CASE,2010,26(6):283-286.(in Chinese)
24 YUE Z,TEATER C,MACLELLAN J,et al.Development of a new bioethanol feedstock-anaerobically digested fiber from confined dairy operations using different digestion configurations[J].Biomass and Bioenergy,2011,35(5):1946-1953.