摘要
为保持杂种白杨717-1B4的优良性状,解决生根困难瓶颈,建立了生理年龄一致性和容器化生根炼苗的组织培养繁育技术。结果表明:外植体为母株根蘖的根际萌芽,以有效氯1.0%的乙氰脲酸钠溶液灭菌25 min,接种在MS培养基上效果最好,再生不定芽达75.91%;以再生不定芽3个以上的外植体进行继代培养,继代周期为30 d,平均再生不定芽12.2个;外植体再生的不定芽扩繁1次转移至营养钵基质上生根和练苗,增殖系数达3.73,增殖培养基为MS+6-BA0.5 mg/L+NAA 0.2 mg/L;生根培养基中IBA为2.0 mg/L生根最好,生根率达98.89%;揭开培养瓶盖驯化小植株容器苗,使之适应自然环境,驯化成活率达92.5%。
In order to maintain the maternal excellent features in offspring and refrain from difficult rooting in vegetative propagation of hybrid aspen,established a juvenility and container rooting-hardening micropropagation system in the present work. The result showed that explants taken from the sprouting between root-shoot junctions of maternal plants,sterilized by soaked 1. 0% effective chlorine of sodium dichloroisocyanurate( Na DCC) solution for25 min,cultured on MS medium,75. 91% of them regenerated juvenile shoots. The explants regenerated more than3 juvenile shoots were selected and sub-cultured on the same fresh medium in each 30 days,each explant produced12. 2 shoots on average. The shoots from explants multiplicated one time before did container rooting and hardening.The shoots increased 3. 73 times on MS medium containing 6-BA 0. 5 mg/L and NAA 0. 2 mg/L,respectively. The rooting rate reached the highest at 98. 89% when IBA was 2. 0 mg/L in rooting medium. The container plantlets hardened in reversion and acclimatization by shifting and opening gradually the tissue culture bottles. The container rooted aspen plants survived at 92. 5% in the acclimatization stage.
引文
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