黄腐酸微生物菌剂对连作平邑甜茶光合特性的影响
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摘要
以盆栽平邑甜茶(Malus hupehensis)幼苗为试材,设置不同黄腐酸微生物菌剂浓度(0、5、10、15 g·kg~(-1)),测定幼苗生物量、叶绿素含量、气体交换参数、叶绿素荧光参数,探讨不同浓度的黄腐酸微生物菌剂对连作平邑甜茶幼苗光合及叶绿素荧光特性的影响。结果表明,连作土壤中添加10 g·kg~(-1)黄腐酸微生物菌剂可显著促进平邑甜茶幼苗的生长,提高根系抗氧化酶活性和叶片叶绿素含量;黄腐酸微生物菌剂能有效缓解因连作障碍引起的叶片净光合速率(P_n)、最大光能转化效率(F_v/F_m)、实际光化学效率(Φ_(PSII))、光化学淬灭系数(q_P)、单位面积有活性反应中心数量(RC/CS_m)等光合和荧光参数的下降,改变OJIP曲线形状。综上,适宜浓度的黄腐酸微生物菌剂能显著提高叶绿素含量和P_n,改善平邑甜茶幼苗叶片的能量分配,减轻连作障碍对平邑甜茶幼苗叶片光系统II(PSII)的破坏,促进幼苗生长。
In order to investigate the effects of fulvic acid on photosynthesis and chlorophyll fluorescence characteristics of Malus hupehensis seedlings under replant condition.The growth,chlorophyll content,gas exchange parameters and chlorophyll fluorescence parameters of M.hupehensis seedlings were studied.A pot experiment was carried out in 2016,designed 4 different concentrations of fulvic acid(0,5,10 and 15 g·kg~(-1)).The results showed that fulvic acid with 10 g·kg~(-1 )could significantly promote the growth of M.hupehensis seedlings,increase root antioxidant enzymes activity and the content of chlorophyll in leaves.Fulvic acid treatment could effectively alleviate the decrease of fluorescence parameters caused by the replant disease,such as photosynthetic rate(P_n),the maximum photochemical efficiency of PSII(F_v/F_m),the actual photochemical efficiency(Φ_(PSII)),the photochemical quenching(q_P)and the number of reactive centers per unit area(RC/CS_m),and change the OJIP curve shape.In conclusion,fulvic acid with suitable concentration could promote the seedlings growth,increase the content of chlorophyll and net photosynthetic rate,improve the energy distribution of M.hupehensis seedling and alleviate the damage of PSII caused by the replant disease.
In order to investigate the effects of fulvic acid on photosynthesis and chlorophyll fluorescence characteristics of Malus hupehensis seedlings under replant condition.The growth,chlorophyll content,gas exchange parameters and chlorophyll fluorescence parameters of M.hupehensis seedlings were studied.A pot experiment was carried out in 2016,designed 4 different concentrations of fulvic acid(0,5,10 and 15 g·kg~(-1)).The results showed that fulvic acid with 10 g·kg~(-1 )could significantly promote the growth of M.hupehensis seedlings,increase root antioxidant enzymes activity and the content of chlorophyll in leaves.Fulvic acid treatment could effectively alleviate the decrease of fluorescence parameters caused by the replant disease,such as photosynthetic rate(P_n),the maximum photochemical efficiency of PSII(F_v/F_m),the actual photochemical efficiency(Φ_(PSII)),the photochemical quenching(q_P)and the number of reactive centers per unit area(RC/CS_m),and change the OJIP curve shape.In conclusion,fulvic acid with suitable concentration could promote the seedlings growth,increase the content of chlorophyll and net photosynthetic rate,improve the energy distribution of M.hupehensis seedling and alleviate the damage of PSII caused by the replant disease.
引文
Bj?rkman O,Demmig B(1987).Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77Kamong vascular plants of diverse origins.Planta,170(4):489-504
Canellas LP,Olivares FL,Aguiar NO,et al(2015a).Humic and fulvic acids as biostimulants in horticulture.Sci Hortic,196:15-27
Canellas LP,Silva SF,Olk D,et al(2015b).Foliar application of herbaspirillum seropedicae and humic acid increase maize yields.J Food Agric Environ,13(1):146-153
Chen XS,Mao ZQ,Shen X,et al(2017).The breeding of new variety of easy colouring functional apple and non-bag cultivation technology.Chin Fruit,(1):1-5(in Chinese with English abstract)[陈学森,毛志泉,沈向等(2017).易着色功能型苹果新品种培育及无袋化栽培技术.中国果树,(1):1-5]
Chen Z,Hou BH,Wang WX,et al(2016).Inhibition of fulvic acid to Valsa mali and its effect on the activity of defense enzyme in apple tree.Plant Protec,42(3):81-86(in Chinese with English abstract)[陈臻,侯宝宏,王卫雄等(2016).黄腐酸处理对苹果树腐烂病菌的抑制作用及对苹果树防御酶活性的影响.植物保护,42(3):81-86]
Coilatz GD(1977).Influence of certain environment factors on photorespiration in Simmondisia chinensis.Planta,134(1):127-132
Dou S,Li Y,Guan S,et al(2016).The structural distinctiveness of humic substances and its formation mechanism in simulated incubation.Acta Pedol Sin,53(4):821-831(in Chinese with English abstract)[窦森,李艳,关松等(2016).腐殖物质特异性及其产生机制.土壤学报,53(4):821-831]
García AC,Berbara RLL,Farías LP,et al(2012).Humic acids of vermicompost as an ecological pathway to increase resistance of rice seedlings to water stress.Afric J of Biotechnol,11(13):3125-3134
Hui ZL,Li CZ,Shi WX,et al(2013).A study on the use of fulvic acid to improve growth and resistance cropping of potato.Acta Pratac Sin,22(4):130-136(in Chinese with English abstract)[回振龙,李朝周,史文煊等(2013).黄腐酸改善连作马铃薯生长发育及抗性生理的研究.草业学报,22(4):130-136]
Li AM,Zhang L,Zhang C,et al(2017).Effects of fulvic acid and betaine pretreatment on physiological characteristics and photosynthesis of Malus hupehensis under drought stress.Acta Bot Boreal-Occident Sin,37(2):307-314(in Chinese with English abstract)[李爱梅,张玲,张超等(2017).黄腐酸和甜菜碱预处理对干旱胁迫下平邑甜茶生理特性及光合的影响.西北植物学报,37(2):307-314]
Li MY,Chao JG,Gu W,et al(2015).Effects of high temperature stress on chlorophyll fluorescence characteristics of Atractylodes lancea from different habitats.Plant Physiol J,51(11):1861-1866(in Chinese with English abstract)[李孟洋,巢建国,谷巍等(2015).高温胁迫对不同产地茅苍术开花前叶片叶绿素荧光特征的影响.植物生理学报,51(11):1861-1866]
Li PM,Gao HY,Strasser RJ(2005).Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study.J Plant Physiol Mol Biol,31(6):559-566(in Chinese with English abstract)[李鹏民,高辉远,Strasser RJ(2005).快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学学报,31(6):559-566]
Loffredo E,Berloco M,Casulli F,et al(2007).In vitro assessment of the inhibition of humic substances on the growth of two strains of Fusarium oxysporum.Biol Ferti Soils,43(6):759-769
Lu QJ,Chao JG,Gu W,et al(2017).Effects of different nitrogen levels on photosynthetic characteristics and physiological indexes of Atractylodes lancea.Plant Physiol J,53(9):1673-1679(in Chinese with English abstract)[陆奇杰,巢建国,谷巍等(2017).不同氮素水平对茅苍术光合特性及生理指标的影响.植物生理学报,53(9):1673-1679]
Luo YY,Zhao XY,Zhang JH,et al(2015).Photosynthesis of Digitaria ciliaris during repeated soil drought and rewatering.Sci Cold Arid Regi,7(1):81-87
Mazzola M,Manici LM(2012).Apple replant disease:role of microbial ecology in cause and control.Ann Rev Phytopathol,50:45-65
Meng P,An YN,Bai XF(2016).Photosynthetic characteristics and chlorophyll a fluorescence induction parameters of Pinus densiflora on sandy soil.Acta Ecol Sin,36(11):3469-3478(in Chinese with English abstract)[孟鹏,安宇宁,白雪峰(2016).沙地赤松光合及叶绿素a快相荧光动力学特性.生态学报,36(11):3469-3478]
Omran RG(1980).Peroxide levels and the activities of catalase,peroxidase,and indoleacetic acid oxidase during and after chilling cucumber seedlings.Plant Physiol,65(2):407-408
Shakeel AA(2011).Exogenous application of some potential growth regulators for improving drough tolerance in maize and soybean(dissertation).Chongqing:Southwest University(in Chinese with English abstract)[Shakeel AA(2011).外源性生长调节物质对玉米和大豆抗旱性的影响(学位论文).重庆:西南大学]
Singh BK,Sharma SR,Singh B(2010).Antioxidant enzymes in cabbage:variability and inheritance of superoxide dismutase,peroxidase and catalase.Sci Hortic,124(1):9-13
Tewoldemedhin YT,Mazzola M,Botha WJ,et al(2011).Characterization of fungi(Fusarium and Rhizoctonia)and oomycetes(Phytophthora and Pythium)associated with apple orchards in South Africa.Eur J Plant Pathol,130:215-229
Wang CB,Wu ZF,Cheng B(2007).Effect of continuous cropping on photosynthesis and metabolism of reactive oxygen in peanut.Acta Agro Sin,33(8):1304-1309(in Chinese with English abstract)[王才斌,吴正锋,成波等(2007).连作对花生光合特性和活性氧代谢的影响.作物学报,33(8):1304-1309]
Wang M,Jiang WT,Sun SY,et al(2017).Effects of appropriate N and P fertilizer on growth and soil environments of replanted apple(Malus hupehensis)seedlings.Plant Physiol J,53(9):1687-1694(in Chinese with English abstract)[王玫,姜伟涛,孙申义等(2017).添加适宜氮磷对连作平邑甜茶幼苗生长及土壤环境的影响.植物生理学报,53(9):1687-1694]
Yim B,Smalla K,Winkelmann T(2013).Evaluation of apple replant problems based on different soil disinfection treatments-links to soil microbial community structure.Plant Soil,366(1-2):617-631
Yin CM,Wang M,Wang JY,et al(2017).The research advance on apple replant disease.Acta Hortic Sin,44(11):2215-2230(in Chinese with English abstract)[尹承苗,王玫,王嘉艳等(2017).苹果连作障碍研究进展.园艺学报,44(11):2215-2230]
Zhang HH,Jin W,Yue BB,et al(2013).Effects of continuous cropping on growth and photosynthetic characteristics of flue-cured tobacco in Northeast China.Acta Tabacco Sin,34(2):18-22(in Chinese with English abstract)[张会慧,金微微,岳冰冰等(2013).连作对东北地区烤烟生长和叶片光合特性的影响.中国烟草科学,34(2):18-22]
Zhang L(2016).Effect of spraying fulvic acid and glyeinebetaine on drought resistant physiology as well as fruit yeild and quality of two malus species(dissertation).Yangling,Shaanxi:Northwest A&F University(in Chinese with English abstract)[张玲(2016).黄腐酸和甜菜碱对苹果抗旱生理及果实产量品质的影响(学位论文).陕西杨凌:西北农林科技大学]
Zhang SQ,Yuan L,Lin ZA,et al(2017).Advances in humic acid for promoting plant growth and its mechanism.Plant Nutr Fert Sci,23(4):1065-1076(in Chinese with English abstract)[张水勤,袁亮,林治安等(2017).腐植酸促进植物生长的机理研究进展.植物营养与肥料学报,23(4):1065-1076]
Zhang Y,Fan T,Jia W,et al(2012).Identification and characterization of a Bacillus subtilis strain TS06 as bio-control agent of strawberry replant disease(Fusarium and Verticilium wilts).Africa J Biotechnol,11(3):570-580
Zhang YK,Han XJ,Chen XL,et al(2009).Exogenous nitric oxide on antioxidative system and ATPase activities from tomato seedlings under copper stress.Sci Hortic,123:217-223
Zhao LY,Deng XL,Shan L(2007).Effects of altered water condition on some chlorophyll fluorescence parameters of flag leaves of winter wheat.Chin J Eco-Agri,15(1):63-66(in Chinese with English abstract)[赵丽英,邓西平,山仑(2007).不同水分处理下冬小麦旗叶叶绿素荧光参数的变化研究.中国生态农业学报,15(1):63-66]
Zhao SJ,Shi GA,Dong XC(2002).Plant Physiology Experiment.Beijing:China Agricultural Science and Technology Press,98-99(in Chinese)[赵世杰,史国安,董新纯(2002).植物生理学实验指导.北京:中国农业科技出版社,98-99]
Canellas LP,Olivares FL,Aguiar NO,et al(2015a).Humic and fulvic acids as biostimulants in horticulture.Sci Hortic,196:15-27
Canellas LP,Silva SF,Olk D,et al(2015b).Foliar application of herbaspirillum seropedicae and humic acid increase maize yields.J Food Agric Environ,13(1):146-153
Chen XS,Mao ZQ,Shen X,et al(2017).The breeding of new variety of easy colouring functional apple and non-bag cultivation technology.Chin Fruit,(1):1-5(in Chinese with English abstract)[陈学森,毛志泉,沈向等(2017).易着色功能型苹果新品种培育及无袋化栽培技术.中国果树,(1):1-5]
Chen Z,Hou BH,Wang WX,et al(2016).Inhibition of fulvic acid to Valsa mali and its effect on the activity of defense enzyme in apple tree.Plant Protec,42(3):81-86(in Chinese with English abstract)[陈臻,侯宝宏,王卫雄等(2016).黄腐酸处理对苹果树腐烂病菌的抑制作用及对苹果树防御酶活性的影响.植物保护,42(3):81-86]
Coilatz GD(1977).Influence of certain environment factors on photorespiration in Simmondisia chinensis.Planta,134(1):127-132
Dou S,Li Y,Guan S,et al(2016).The structural distinctiveness of humic substances and its formation mechanism in simulated incubation.Acta Pedol Sin,53(4):821-831(in Chinese with English abstract)[窦森,李艳,关松等(2016).腐殖物质特异性及其产生机制.土壤学报,53(4):821-831]
García AC,Berbara RLL,Farías LP,et al(2012).Humic acids of vermicompost as an ecological pathway to increase resistance of rice seedlings to water stress.Afric J of Biotechnol,11(13):3125-3134
Hui ZL,Li CZ,Shi WX,et al(2013).A study on the use of fulvic acid to improve growth and resistance cropping of potato.Acta Pratac Sin,22(4):130-136(in Chinese with English abstract)[回振龙,李朝周,史文煊等(2013).黄腐酸改善连作马铃薯生长发育及抗性生理的研究.草业学报,22(4):130-136]
Li AM,Zhang L,Zhang C,et al(2017).Effects of fulvic acid and betaine pretreatment on physiological characteristics and photosynthesis of Malus hupehensis under drought stress.Acta Bot Boreal-Occident Sin,37(2):307-314(in Chinese with English abstract)[李爱梅,张玲,张超等(2017).黄腐酸和甜菜碱预处理对干旱胁迫下平邑甜茶生理特性及光合的影响.西北植物学报,37(2):307-314]
Li MY,Chao JG,Gu W,et al(2015).Effects of high temperature stress on chlorophyll fluorescence characteristics of Atractylodes lancea from different habitats.Plant Physiol J,51(11):1861-1866(in Chinese with English abstract)[李孟洋,巢建国,谷巍等(2015).高温胁迫对不同产地茅苍术开花前叶片叶绿素荧光特征的影响.植物生理学报,51(11):1861-1866]
Li PM,Gao HY,Strasser RJ(2005).Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study.J Plant Physiol Mol Biol,31(6):559-566(in Chinese with English abstract)[李鹏民,高辉远,Strasser RJ(2005).快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学学报,31(6):559-566]
Loffredo E,Berloco M,Casulli F,et al(2007).In vitro assessment of the inhibition of humic substances on the growth of two strains of Fusarium oxysporum.Biol Ferti Soils,43(6):759-769
Lu QJ,Chao JG,Gu W,et al(2017).Effects of different nitrogen levels on photosynthetic characteristics and physiological indexes of Atractylodes lancea.Plant Physiol J,53(9):1673-1679(in Chinese with English abstract)[陆奇杰,巢建国,谷巍等(2017).不同氮素水平对茅苍术光合特性及生理指标的影响.植物生理学报,53(9):1673-1679]
Luo YY,Zhao XY,Zhang JH,et al(2015).Photosynthesis of Digitaria ciliaris during repeated soil drought and rewatering.Sci Cold Arid Regi,7(1):81-87
Mazzola M,Manici LM(2012).Apple replant disease:role of microbial ecology in cause and control.Ann Rev Phytopathol,50:45-65
Meng P,An YN,Bai XF(2016).Photosynthetic characteristics and chlorophyll a fluorescence induction parameters of Pinus densiflora on sandy soil.Acta Ecol Sin,36(11):3469-3478(in Chinese with English abstract)[孟鹏,安宇宁,白雪峰(2016).沙地赤松光合及叶绿素a快相荧光动力学特性.生态学报,36(11):3469-3478]
Omran RG(1980).Peroxide levels and the activities of catalase,peroxidase,and indoleacetic acid oxidase during and after chilling cucumber seedlings.Plant Physiol,65(2):407-408
Shakeel AA(2011).Exogenous application of some potential growth regulators for improving drough tolerance in maize and soybean(dissertation).Chongqing:Southwest University(in Chinese with English abstract)[Shakeel AA(2011).外源性生长调节物质对玉米和大豆抗旱性的影响(学位论文).重庆:西南大学]
Singh BK,Sharma SR,Singh B(2010).Antioxidant enzymes in cabbage:variability and inheritance of superoxide dismutase,peroxidase and catalase.Sci Hortic,124(1):9-13
Tewoldemedhin YT,Mazzola M,Botha WJ,et al(2011).Characterization of fungi(Fusarium and Rhizoctonia)and oomycetes(Phytophthora and Pythium)associated with apple orchards in South Africa.Eur J Plant Pathol,130:215-229
Wang CB,Wu ZF,Cheng B(2007).Effect of continuous cropping on photosynthesis and metabolism of reactive oxygen in peanut.Acta Agro Sin,33(8):1304-1309(in Chinese with English abstract)[王才斌,吴正锋,成波等(2007).连作对花生光合特性和活性氧代谢的影响.作物学报,33(8):1304-1309]
Wang M,Jiang WT,Sun SY,et al(2017).Effects of appropriate N and P fertilizer on growth and soil environments of replanted apple(Malus hupehensis)seedlings.Plant Physiol J,53(9):1687-1694(in Chinese with English abstract)[王玫,姜伟涛,孙申义等(2017).添加适宜氮磷对连作平邑甜茶幼苗生长及土壤环境的影响.植物生理学报,53(9):1687-1694]
Yim B,Smalla K,Winkelmann T(2013).Evaluation of apple replant problems based on different soil disinfection treatments-links to soil microbial community structure.Plant Soil,366(1-2):617-631
Yin CM,Wang M,Wang JY,et al(2017).The research advance on apple replant disease.Acta Hortic Sin,44(11):2215-2230(in Chinese with English abstract)[尹承苗,王玫,王嘉艳等(2017).苹果连作障碍研究进展.园艺学报,44(11):2215-2230]
Zhang HH,Jin W,Yue BB,et al(2013).Effects of continuous cropping on growth and photosynthetic characteristics of flue-cured tobacco in Northeast China.Acta Tabacco Sin,34(2):18-22(in Chinese with English abstract)[张会慧,金微微,岳冰冰等(2013).连作对东北地区烤烟生长和叶片光合特性的影响.中国烟草科学,34(2):18-22]
Zhang L(2016).Effect of spraying fulvic acid and glyeinebetaine on drought resistant physiology as well as fruit yeild and quality of two malus species(dissertation).Yangling,Shaanxi:Northwest A&F University(in Chinese with English abstract)[张玲(2016).黄腐酸和甜菜碱对苹果抗旱生理及果实产量品质的影响(学位论文).陕西杨凌:西北农林科技大学]
Zhang SQ,Yuan L,Lin ZA,et al(2017).Advances in humic acid for promoting plant growth and its mechanism.Plant Nutr Fert Sci,23(4):1065-1076(in Chinese with English abstract)[张水勤,袁亮,林治安等(2017).腐植酸促进植物生长的机理研究进展.植物营养与肥料学报,23(4):1065-1076]
Zhang Y,Fan T,Jia W,et al(2012).Identification and characterization of a Bacillus subtilis strain TS06 as bio-control agent of strawberry replant disease(Fusarium and Verticilium wilts).Africa J Biotechnol,11(3):570-580
Zhang YK,Han XJ,Chen XL,et al(2009).Exogenous nitric oxide on antioxidative system and ATPase activities from tomato seedlings under copper stress.Sci Hortic,123:217-223
Zhao LY,Deng XL,Shan L(2007).Effects of altered water condition on some chlorophyll fluorescence parameters of flag leaves of winter wheat.Chin J Eco-Agri,15(1):63-66(in Chinese with English abstract)[赵丽英,邓西平,山仑(2007).不同水分处理下冬小麦旗叶叶绿素荧光参数的变化研究.中国生态农业学报,15(1):63-66]
Zhao SJ,Shi GA,Dong XC(2002).Plant Physiology Experiment.Beijing:China Agricultural Science and Technology Press,98-99(in Chinese)[赵世杰,史国安,董新纯(2002).植物生理学实验指导.北京:中国农业科技出版社,98-99]