植保抗生素阿扎霉素对白菜根肿病的防治效果
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摘要
采用盆栽试验和田间试验相结合的方法,测定了12.5%阿扎霉素水剂不同质量浓度对白菜根肿病的防治效果。试验结果表明:采用12.5%阿扎霉素水剂500~1 000倍,在大白菜播种后5d第一次用药,之后每间隔7d施药1次,共施药3次,温室盆栽试验最高防治效果为87.58%,田间试验最高防治效果为80.62%,其与对照药剂500g/L氟啶胺悬浮剂的防治效果间差异显著。植保抗生素12.5%阿扎霉素水剂对白菜根肿病具有较好的防治效果,具备开发成防治根肿病新型杀菌剂的潜力。
In order to provide guidance for the development of azalomycin,control effect of azalomycin 12.5% AS to Plasmodiophora brassicae were carried out under greenhouse and field conditions.The results showed that azalomycin had a good control effect to clubroot disease.The optimum concentration was 500 times-1 000 times.The optimum first using period was 5 days after sowing,and then applied every 7 dfor 3 times.The maximum control effect in greenhouse pot experiment was 87.58%,while that in field experiment was 80.62%.The control effect of azalomycin to Plasmodiophora brassicae was significantly different from fluazinam 500 g/L SC.Azalomycin has the potential to develop into new fungicide for clubroot disease control.
In order to provide guidance for the development of azalomycin,control effect of azalomycin 12.5% AS to Plasmodiophora brassicae were carried out under greenhouse and field conditions.The results showed that azalomycin had a good control effect to clubroot disease.The optimum concentration was 500 times-1 000 times.The optimum first using period was 5 days after sowing,and then applied every 7 dfor 3 times.The maximum control effect in greenhouse pot experiment was 87.58%,while that in field experiment was 80.62%.The control effect of azalomycin to Plasmodiophora brassicae was significantly different from fluazinam 500 g/L SC.Azalomycin has the potential to develop into new fungicide for clubroot disease control.
引文
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[4]DU J,LUO X W,PENG J,et al.Chinese cabbage infected by Plasmodiophora brassicae was first identified in Changde,China[C]∥Sustainable Development:Proceedings of the 2015International Conference on Sustainable Development(ICSD2015).World Scientific,2016:3-9.
[5]CHAI A L,XIE X W,SHI Y X,et al.Research status of clubroot(Plasmodiophora brassicae)on cruciferous crops in China[J].Canadian Journal of Plant Pathology,2014,36(S1):142-153.
[6]陈瑶,王火旭.十字花科根肿病的研究进展[J].天津农业科学,2014,20(4):77-79.
[7]DIXON G R.Managing clubroot disease(caused by Plasmodiophora brassicae Wor.)by exploiting the interactions between calcium cyanamide fertilizer and soil microorganisms[J].The Journal of Agricultural Science,2017,155(4):527-543.
[8]SCHULLER A,LUDWIG-MLLER J.Histological methods to detect the clubroot pathogen Plasmodiophora brassicae during its complex life cycle[J].Plant Pathology,2016,65(8):1223-1237.
[9]季海雯,任莉,陈坤荣,等.油菜根肿病病原主要生理小种和品种抗病性鉴定[J].中国油料作物学报,2013,35(3):301-306.
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[11]杨佩文,赵江源,李铭刚,等.马来西亚链霉菌ECO-00002产生的阿扎霉素F3、阿扎霉素F4和尼菲霉素:分离纯化、结构解析及其抗植物病原真菌活性[C]∥第八届全国新农药创制学术交流会论文集.杭州:中化化工科学技术研究总院,2009:352-362.
[12]陶伟林,樊国昌,周娜,等.高山甘蓝根肿病田间防效试验初报[J].南方农业,2011,5(7):12-14.
[13]ARAI M,HAMANO K.Isolation of three main components,F3,F4and F5,from azalomycin F-complex[J].The Journal of Antibiotics,1970,23(3):107-112.
[14]CHANDRA A,NAIR M G.Azalomycin F complex from Streptomyces hygroscopicus,MSU/MN-4-75B[J].The Journal of Antibiotics,1995,48(8):896-898.
[15]CHENG J,YANG S H,PALANIYANDI S A,et al.Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968isolated from agricultural soil[J].Journal of the Korean Society for Applied Biological Chemistry,2010,53(5):545-552.
[16]BERLINCK R G S.Natural guanidine derivatives[J].Natural Product Reports,1999,16(3):339-365.
[17]KUMAZAWA S,ASAMI Y,AWANE K,et al.Structural studies of new macrolide antibiotics,shurimycins A and B[J].The Journal of Antibiotics,1994,47(6):688-696.
[18]KIM H Y,KIM J D,HONG J S,et al.Identification of antifungal niphimycin fromStreptomyces sp.KP6107by screening based on adenylate kinase assay[J].Journal of Basic Microbiology,2013,53(7):581-589.
[19]YUAN G,HONG K,LIN H,et al.New azalomycin F analogs from mangrove Streptomyces sp.211726with activity against microbes and cancer cells[J].Marine Drugs,2013,11(3):817-829.
[20]YUAN G,LIN H,WANG C,et al.1 H and 13C assignments of two new macrocyclic lactones isolated from Streptomyces sp.211726and revised assignments of azalomycin F3a,F4aand F5a[J].Magnetic Resonance in Chemistry,2011,49(1):30-37.
[2]PENG G,LAHLALI R,HWANG S F,et al.Crop rotation,cultivar resistance,and fungicides/biofungicides for managing clubroot(Plasmodiophora brassicae)on canola[J].Canadian Journal of Plant Pathology,2014,36(S1):99-112.
[3]HWANG S F,HOWARD R J,STRELKOV S E,et al.Management of clubroot(Plasmodiophora brassicae)on canola(Brassica napus)in western Canada[J].Canadian Journal of Plant Pathology,2014,36(S1):49-65.
[4]DU J,LUO X W,PENG J,et al.Chinese cabbage infected by Plasmodiophora brassicae was first identified in Changde,China[C]∥Sustainable Development:Proceedings of the 2015International Conference on Sustainable Development(ICSD2015).World Scientific,2016:3-9.
[5]CHAI A L,XIE X W,SHI Y X,et al.Research status of clubroot(Plasmodiophora brassicae)on cruciferous crops in China[J].Canadian Journal of Plant Pathology,2014,36(S1):142-153.
[6]陈瑶,王火旭.十字花科根肿病的研究进展[J].天津农业科学,2014,20(4):77-79.
[7]DIXON G R.Managing clubroot disease(caused by Plasmodiophora brassicae Wor.)by exploiting the interactions between calcium cyanamide fertilizer and soil microorganisms[J].The Journal of Agricultural Science,2017,155(4):527-543.
[8]SCHULLER A,LUDWIG-MLLER J.Histological methods to detect the clubroot pathogen Plasmodiophora brassicae during its complex life cycle[J].Plant Pathology,2016,65(8):1223-1237.
[9]季海雯,任莉,陈坤荣,等.油菜根肿病病原主要生理小种和品种抗病性鉴定[J].中国油料作物学报,2013,35(3):301-306.
[10]彭丽莎,袁天成,李成琼,等.十字花科作物根肿病生防菌研究进展[J].植物保护,2015,41(4):16-22.
[11]杨佩文,赵江源,李铭刚,等.马来西亚链霉菌ECO-00002产生的阿扎霉素F3、阿扎霉素F4和尼菲霉素:分离纯化、结构解析及其抗植物病原真菌活性[C]∥第八届全国新农药创制学术交流会论文集.杭州:中化化工科学技术研究总院,2009:352-362.
[12]陶伟林,樊国昌,周娜,等.高山甘蓝根肿病田间防效试验初报[J].南方农业,2011,5(7):12-14.
[13]ARAI M,HAMANO K.Isolation of three main components,F3,F4and F5,from azalomycin F-complex[J].The Journal of Antibiotics,1970,23(3):107-112.
[14]CHANDRA A,NAIR M G.Azalomycin F complex from Streptomyces hygroscopicus,MSU/MN-4-75B[J].The Journal of Antibiotics,1995,48(8):896-898.
[15]CHENG J,YANG S H,PALANIYANDI S A,et al.Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968isolated from agricultural soil[J].Journal of the Korean Society for Applied Biological Chemistry,2010,53(5):545-552.
[16]BERLINCK R G S.Natural guanidine derivatives[J].Natural Product Reports,1999,16(3):339-365.
[17]KUMAZAWA S,ASAMI Y,AWANE K,et al.Structural studies of new macrolide antibiotics,shurimycins A and B[J].The Journal of Antibiotics,1994,47(6):688-696.
[18]KIM H Y,KIM J D,HONG J S,et al.Identification of antifungal niphimycin fromStreptomyces sp.KP6107by screening based on adenylate kinase assay[J].Journal of Basic Microbiology,2013,53(7):581-589.
[19]YUAN G,HONG K,LIN H,et al.New azalomycin F analogs from mangrove Streptomyces sp.211726with activity against microbes and cancer cells[J].Marine Drugs,2013,11(3):817-829.
[20]YUAN G,LIN H,WANG C,et al.1 H and 13C assignments of two new macrocyclic lactones isolated from Streptomyces sp.211726and revised assignments of azalomycin F3a,F4aand F5a[J].Magnetic Resonance in Chemistry,2011,49(1):30-37.