白芍提取物对罗非鱼氧化损伤的保护作用
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摘要
为了解白芍提取物对罗非鱼(Oreochromis niloticus)肝损伤和炎症反应的保护作用,将罗非鱼随机分为5组,空白对照组(Ctrl组)、H_2O_2组、白芍提取物组(0.5、1.0、5 g/kg),饲喂60 d后,用H_2O_2处理24 h,在H_2O_2处理前后,分别采集血液、肝、鳃和肌组织,并测定肝损伤指标、氧化应激参数和基因表达。结果显示:白芍提取物显著提高了血清、肝和鳃的抗氧化能力。氧化损伤实验中,白芍提取物不同程度地降低了谷丙转氨酶(GPT)、谷草转氨酶(GOT)和丙二醛(MDA)水平;提高了超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和总抗氧化能力(T-AOC)水平;上调了肿瘤坏死因子α(TNF-α)、白细胞介素IL-1β和IL-8基因表达;下调了IL-10基因表达。以上结果表明,白芍提取物可提高罗非鱼抗氧化能力,抑制氧化应激引起的肝损伤和炎症反应。
The experimental fish were randomly divided into 5 groups and feeding for 60 days to understand the protective effects of paeoniae alba radix extract(PARE)on liver oxidative stress in tilapia(Oreochromis niloticus),including normal control group,H_2O_(2 ) group,and three PARE groups(0.5,1.0 and 5 g/kg).Then the blood and liver were collected to assay biochemical parameters and genes expression.The results showed that PARE significantly improved the antioxidant capacity of serum,liver and gill.In the oxidative stress damage experiment,PARE inhibited the increase of glutamate pyruvate transaminase(GPT),glutamate oxalate transaminase(GOT)and liver malondialdehyde(MDA)formation,increasing superoxide dismutase(SOD),glutathione(GSH)and total antioxidant capacity(T-AOC)levels to varying degrees.Meanwhile,PARE significantly up-regulated the tumor necrosis factor α(TNF-α),Interleukin 1β(IL-1β)and interleukin IL-8(IL-8)gene expression,and down-regulated interleukin IL-10(IL-10)gene expression in liver tissue.In conclusion,the above data shows PARE can increase the antioxidant capacity of tilapia,inhibit liver damage and inflammatory response caused by oxidative stress.
The experimental fish were randomly divided into 5 groups and feeding for 60 days to understand the protective effects of paeoniae alba radix extract(PARE)on liver oxidative stress in tilapia(Oreochromis niloticus),including normal control group,H_2O_(2 ) group,and three PARE groups(0.5,1.0 and 5 g/kg).Then the blood and liver were collected to assay biochemical parameters and genes expression.The results showed that PARE significantly improved the antioxidant capacity of serum,liver and gill.In the oxidative stress damage experiment,PARE inhibited the increase of glutamate pyruvate transaminase(GPT),glutamate oxalate transaminase(GOT)and liver malondialdehyde(MDA)formation,increasing superoxide dismutase(SOD),glutathione(GSH)and total antioxidant capacity(T-AOC)levels to varying degrees.Meanwhile,PARE significantly up-regulated the tumor necrosis factor α(TNF-α),Interleukin 1β(IL-1β)and interleukin IL-8(IL-8)gene expression,and down-regulated interleukin IL-10(IL-10)gene expression in liver tissue.In conclusion,the above data shows PARE can increase the antioxidant capacity of tilapia,inhibit liver damage and inflammatory response caused by oxidative stress.
引文
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[22]秦亚东,钟正灵,汪荣斌,等.白芍多糖对D-半乳糖胺/脂多糖诱导小鼠急性肝损伤的保护作用[J].中国临床药理学与治疗学,2015,20(8):854-858.
[23]Ohkawa H,Ohishi N,Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J].Anal Biochem,1979,95(2):351-358.
[24]Kim H Y,Park J,Lee K H,et al.Ferulic acid protects against carbon tetrachloride-induced liver injury in mice[J].Toxicology,2011,282(3):104-111.
[25] Dan X M,Zhang T W,Li Y W,et al.Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine[J].Fish Shellfish Immun,2013,34(3):885-891.
[26] Domitrovi■ R,Jakovac H,Blagojevi■ G.Hepatoprotective activity of berberine is mediated by inhibition of TNF-α,COX-2,and iNOS expression in CCl(4)-intoxicated mice[J].Toxicology,2011,280(1):33-43.
[27]Hawrylowicz C W.Regulatory T cells and IL-10 in allergic in flammation [J].J ExP Med,2005,202(11):1459-1463
[28]姚必瑜,黄智铭.黄连素对四氯化碳诱导的小鼠急性肝损伤的保护作用[J].中国现代应用药学,2016,33(4):424-427.
[2]Cabello F C.Heavy use of prophylactic antibiotics in aquaculture:a growing problem for human and animal health and for the environment [J].Environ Microbiol,2010,8(7):1137-1144.
[3] Fu J,Huang H,Liu J,et al.Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis [J].Eur J Pharmacol,2007,568(1):213-221.
[4] Jiang Z H,Lin H J,Yao H D,et al.SelW protects against H2O2-induced liver injury in chickens via inhibiting inflammation and apoptosis[J].Rsc Adv,2017,7(25):15158-15167.
[5]Wang Q,Ju X,Chen Y,et al.Effects of l-carnitine against H2O2-induced oxidative stress in grass carp ovary cells(Ctenopharyngodon idellus)[J].Fish Physiol Biochem,2016,42(3):845-857.
[6]钱续.虹鳟肝损伤的中草药治疗[J].淡水渔业,2002,32,(3):31-32.
[7]刘丽平,薛晖,葛筱琴,等.复方中草药对青虾肌肉和肝胰脏中几种非特异性免疫因子的影响[J].淡水渔业,2007,37(6):11-14.
[8]李斌,唐毅,王志干,等.复方中草药制剂对草鱼肝胆综合症的防治研究[J].南方水产科学,2011,7(2):35-41.
[9]明建华,谢骏,徐跑,等.大黄素、维生素C及其配伍对团头鲂感染嗜水气单胞菌后生理生化指标的影响[J].中国水产科学,2011,18(3):588-601.
[10]陈四平,张浩,李相坤,等.中药白芍的研究进展[J].承德医学院学报,2008,25(3):293-296.
[11]王华,魏伟,岳莉,等.白芍总苷对卡介苗加脂多糖引起的小鼠免疫性肝损伤的保护作用[J].中国药理学通报,2004,20(8):875-878.
[12]詹可顺,王华,魏伟.白芍总苷对小鼠化学性肝损伤的保护作用及机制[J].安徽医科大学学报,2006,41(6):664-666.
[13]陈锡强,彭维兵,侯海荣,等.27种中药提取物抑制血管生成活性的评价[J].山东科学,2013,26(3):21-25.
[15]王庆奎,陈成勋,郭永军,等.7种中草药对斑点叉尾鮰生理生化指标的影响[J].安徽农业科学,2011,39(15):9305-9308.[15] Zheng Y,Zhao Z,Wu W,et al.Effects of dietary resveratrol supplementation on hepatic and serum pro-/anti-inflammatory activity in juvenile GIFT tilapia,Oreochromis niloticus[J].Dev Comp Immunol,2017,73:220-228.
[16]张立婷,何瑜,熊亚星,等.还原型谷胱甘肽对氧化应激所致肝损伤的作用机制[J].湖南中医药大学学报,2009,29(8):15-17.
[17]Lund B O,Miller D M,Woods J S.Studies on Hg(II)-induced H2O2 formation and oxidative stress in vivo and in vitro in rat kidney mitochondria[J].Biochem Pharmacol,1993,45(10):2017-2024.
[18] Rao M V,Paliyath G,Ormrod D P,et al.Influence of salicylic acid on H2O2 production,oxidative stress,and H2O2-metabolizing enzymes.Salicylic acid-mediated oxidative damage requires H2O2[J].Plant Physiol,1997,115(1):137-149.
[19] Mello Filho A C,Hoffmann M E,Meneghi R.Cell killing and DNA damage by hydrogen peroxide are mediated by intracellular iron[J].Biochem J,1984,218:273-275.
[20] Rowley D A,Halliwell B.Superoxide-dependent and ascorbate-dependent formation of hydroxyl radicals in the presence of copper salts:a physiologically significant reaction[J].Arch Biochem Biophys,1983,225:279-284.
[21] Imlay J A,Chin S M,L inn S.Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro[J].Science,1988,240:640-642.
[22]秦亚东,钟正灵,汪荣斌,等.白芍多糖对D-半乳糖胺/脂多糖诱导小鼠急性肝损伤的保护作用[J].中国临床药理学与治疗学,2015,20(8):854-858.
[23]Ohkawa H,Ohishi N,Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J].Anal Biochem,1979,95(2):351-358.
[24]Kim H Y,Park J,Lee K H,et al.Ferulic acid protects against carbon tetrachloride-induced liver injury in mice[J].Toxicology,2011,282(3):104-111.
[25] Dan X M,Zhang T W,Li Y W,et al.Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine[J].Fish Shellfish Immun,2013,34(3):885-891.
[26] Domitrovi■ R,Jakovac H,Blagojevi■ G.Hepatoprotective activity of berberine is mediated by inhibition of TNF-α,COX-2,and iNOS expression in CCl(4)-intoxicated mice[J].Toxicology,2011,280(1):33-43.
[27]Hawrylowicz C W.Regulatory T cells and IL-10 in allergic in flammation [J].J ExP Med,2005,202(11):1459-1463
[28]姚必瑜,黄智铭.黄连素对四氯化碳诱导的小鼠急性肝损伤的保护作用[J].中国现代应用药学,2016,33(4):424-427.