密生雪灵芝的化学成分研究
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摘要
目的研究密生雪灵芝的化学成分。方法利用硅胶柱层析、大孔吸附树脂、中压柱层析、Sephadex LH-20和反相C18半制备液相色谱等手段进行分离纯化,并通过1HNMR、13CNMR等波谱技术来鉴定结构。结果从密生雪灵芝全草的正丁醇萃取物中分离得到了10个化合物,分别鉴定为:小麦黄素(Ⅰ)、小麦黄素-7-O-β-D-葡萄糖苷(Ⅱ)、山奈酚-3-O-α-L-鼠李糖苷(Ⅲ)、槲皮素-3-O-β-D-葡萄糖苷(Ⅳ)、邻苯二甲酸二异丁酯(Ⅴ)、没食子酸(Ⅵ)、山奈酚(Ⅶ)、槲皮素(Ⅷ)、芹菜素(Ⅸ)、杨梅素(Ⅹ)。结论所有化合物均为首次从密生雪灵芝中分离得到。
OBJECTIVE To investigate the chemical components of Arenaria densissima. METHODS Silica gel,macroporous resin,MCI gel,Sephadex LH-20 and reversed phase C18 semi-prep-HPLC were used for the isolation andpurification. Structures of the isolated compounds were identified through1 HNMR and13 CNMR. RESULTS Ten compoundswere isolated from n-butyl alcohol extraction of the whole plant of A. densissima. They were identified as tricin( Ⅰ),tricin-7-O-β-D-glucopyranoside( Ⅱ),kaempferol-3-O-α-L-rhamnopyranoside( Ⅲ),quercetin-3-O-β-D-glucoside(Ⅳ),diisobutyl-phthalate( Ⅴ),gallic acid( Ⅵ),kaempferol( Ⅶ),quercetin( Ⅷ),apigenin( Ⅸ),and myricetin( Ⅹ).CONCLUSION All the compounds are isolated from this plant for the first time.
OBJECTIVE To investigate the chemical components of Arenaria densissima. METHODS Silica gel,macroporous resin,MCI gel,Sephadex LH-20 and reversed phase C18 semi-prep-HPLC were used for the isolation andpurification. Structures of the isolated compounds were identified through1 HNMR and13 CNMR. RESULTS Ten compoundswere isolated from n-butyl alcohol extraction of the whole plant of A. densissima. They were identified as tricin( Ⅰ),tricin-7-O-β-D-glucopyranoside( Ⅱ),kaempferol-3-O-α-L-rhamnopyranoside( Ⅲ),quercetin-3-O-β-D-glucoside(Ⅳ),diisobutyl-phthalate( Ⅴ),gallic acid( Ⅵ),kaempferol( Ⅶ),quercetin( Ⅷ),apigenin( Ⅸ),and myricetin( Ⅹ).CONCLUSION All the compounds are isolated from this plant for the first time.
引文
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[3]伍贤学,谢建新,李德良,等.团状雪灵芝的化学成分研究(Ⅰ)[J].华西药学杂志,2013,28(1):4-5.
[4]伍贤学,黄齐林,谢建新,等.密生雪灵芝的化学成分初步研究(Ⅰ)[J].云南民族大学学报:自然科学版,2012,21(5):313-315.
[5]张沐新,刘银燕,孙薇,等.玉米苞叶中新黄酮类化合物的分离和鉴定[J].高等学校化学学报,2011,32(11):2554-2557.
[6]路小利,乔英,张宪民,等.金鱼藻的化学成分(英文)[J].植物分类与资源学报,2007,29(2):263-264.
[7]崔莹.盘龙七化学成分研究(Ⅱ)[J].中草药,2012,43(9):1704-1707.
[8]李海涛,曹芳,张东梅.唐古特白刺果实的化学成分研究[J].华西药学杂志,2018,33(3):231-234.
[9]牛芸.大皮伞化学成分研究[J].南方林业科学,2013,41(4):6-7.
[10]Nawwar M,Buddrus J,Bauer H.Dimeric phenolic constituents from the roots of Tamarix nilotica[J].Phytochemistry,1982,21(7):1755-1758.
[11]李羽晗,王欣,石继祥,等.柿叶的化学成分研究[J].华西药学杂志,2018,33(4):388-392.
[12]杨伟俊,顾政一,满尔哈巴,等.芳香新塔花中黄酮类成分的研究[J].时珍国医国药,2011,22(2):375-377.
[13]周先礼,赖永新,吴奶珠,等.藏药髯花杜鹃花中化学成分的研究[J].华西药学杂志,2010,25(2):132-134.