纳豆激酶分离纯化和酶活性测定的研究进展
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摘要
该文对纳豆激酶的分离纯化和酶活性测定进行了综述。重点讨论了溶剂沉淀法、柱层析法、磁性微球吸附法、膨胀床法、反相胶束法、三相分割法等分离方法。对酶活性的不同测定方法进行了讨论和比较。提出了将核酸适配体识别技术用于纳豆激酶分离纯化和酶活性测定的可行性。
In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed.
In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed.
引文
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[2] Sumi H, Yanagisawa Y, Yatagai C, et al. Food Sci Technol Res, 2004, 10(1): 17
[3] Cai D B, Zhu C J, Chen S W. World J Microb Bio, 2017, 33: 84
[4] Yang C L, Xing J M, Liu J G, et al. Modern Chemical Industry, 2004, 24(2): 23 阳承利, 邢建民, 刘俊果, 等. 现代化工, 2004, 24(2): 23
[5] Sumi H, Hamada H, Tsushima H, et al. Experientia, 1987, 43(10): 1110
[6] China Industrial Economy News. Chinese Natto Industry Develops Toward Standardization. [2015-04-28]. https://news.21food.cn/35/2419520.html 中国产经新闻报. 中国纳豆产业走向规范化发展轨道. [2015-04-28]. https://news.21food.cn/35/2419520.html
[7] Wang H, Sun X J, Wang L, et al. Ann Microbiol, 2018, 68: 655
[8] Deng B, Lin Y, Wang C, et al. Anal Chim Acta, 2014, 837: 1
[9] Chen H J, McGowan E M, Ren N, et al. Biomarker Insights, 2018, 13: 1
[10] Kurosawa Y, Nirengi S, Homma T, et al. Sci Rep, 2015, 5: 1
[11] Wang X N, Jiang S F, Wang X Y, et al. Asian J Pharm Sci, 2015, 10: 386
[12] Cui W J, Suo F Y, Cheng J T, et al. Microb Biotechnol, 2018, 11: 930
[13] Wei X T, Luo M F, Xie Y C, et al. Appl Biochem Biotechnol, 2012, 168: 1753
[14] Yang Z X, Zhang S M, Zhang Y H, et al. Chinese Journal of Pharmaceutical Biotechnology, 1996, 3(3): 133 杨志兴, 张淑梅, 张云湖, 等. 药物生物技术, 1996, 3(3): 133
[15] Fujita M, Nomura K, Hong K, et al. Biochem Biophys Res Commun, 1993, 197(3): 1340
[16] Yan J Q, Tong Y, Zang Y A. Chinese Journal of Pharmaceutical Biotechnology, 2000, 7(3): 149 阎家麒, 童岩, 藏莹安. 药物生物技术, 2000, 7(3): 149
[17] Liu B Y, Song H Y. Acta Bioch Bioph Sin, 2002, 34(3): 338
[18] Peng Y, Huang Q, Zhang R H. Comp Biochem Phys B, 2003, 134(1): 45
[19] Wang C, Du M, Zheng D M, et al. J Agric Food Chem, 2009, 57: 9722
[20] Wang S L, Wu Y Y, Liang T W. New Biotechnol, 2011, 28(2): 196
[21] Dubey R, Kumar J, Agrawala D. et al. Afr J Biotechnol, 2011, 10: 1408
[22] Lin H T V, Wu G J, Hsieh MC, et al. J Mar Sci Technol, 2015, 23: 240
[23] Li X X, Wang X L, Xiong S L, et al. Biotechnol Lett, 2007, 29: 1459
[24] Deepak V, Pandian S, Kalishwaralal K, et al. BioreIsour Technol, 2009, 100(24): 6644
[25] Yang C, Xing J, Guan Y, et al. Appl Microbiol Biotech, 2006, 72(3): 616
[26] Clemmitt R H, Chase H A. Biotechnol Bioeng, 2000, 67: 206
[27] Hu H, Yao S, Mei L, et al. Biotech Lett, 2000, 22(17): 1383
[28] Lu M, Lin D, Wu Y, et al. Biotech Bioprocess Eng, 2005, 10(2): 128
[29] Liu J G, Xing J M, Chang T S, et al. Bioprocess Biosyst Eng, 2006, 28(4): 267
[30] Garg R, Thorat B. Adv Microb Physiol, 2014, 132: 19
[31] Liang Y B, Bai L, Tang X Y, et al. Farm Products Processing, 2011, 24(4): 23 梁一博, 白亮, 唐鑫媛, 等. 农产品加工, 2011, 24(4): 23
[32] Astrup T, Mullertz S. Arch Biophys, 1952, 40: 346
[33] Nie G J, Zhao R, Yue W J, et al. Science and Technology of Food Industry, 2015, 36(13): 298 聂光军, 赵锐, 岳文瑾, 等. 食品工业科技, 2015, 36(13): 298
[34] Sumi H. J Brew Soc Japan, 1993, 88(6): 482
[35] Wang C, Du M, Zheng D, et al. J Agric Food Chem, 2009, 57(20): 9722
[36] Xie Q L, Lin J. Guangdong Pharmaceutical Journal, 2000, 10(6): 8 谢秋玲, 林剑. 广东药学, 2000, 10(6): 8
[37] Yuki Y, Nakagawa T, Fujita M, et al. Bio Sci Biotech Bioch, 1994, 58(2): 366
[38] Cai D, Wei X, Qiu Y, et al. J Appl Microbiol, 2016, 121(3): 704
[39] Lu M H, Lin D Q, Wu Y C, et al. Biotechnol Bioproc E, 2005, 10(2): 128
[40] Xiong Y X, Yin Z N, Yang C, et al. Chinese Journal of Pharmaceutical Biotechnology, 2006, 13(2): 140 熊迎新, 尹宗宁, 杨超, 等. 药物生物技术, 2006, 13(2): 140
[41] Yang M J, Yang X T, Feng H Q, et al. Food Research and Development, 2008, 29(2): 137 杨明俊, 杨晓彤, 冯慧琴, 等. 食品研究与开发, 2008, 29(2): 137
[42] South Money. Natto Kinase Standards was Formulated, Which will Contain the Market Turmoil. [2018-11-28]. http://www.southmoney.com/caijing/gongsixinwen/2018 11/2775163.html 南方财富网. 纳豆激酶标准进入制订阶段, 市场乱象将得到遏制. [2018-11-28]. http://www.southmoney.com/caijing/gongsixinwen/201811/2775163.html
[43] Deng B, Lin Y, Wang C, et al. Anal Chim Acta, 2014, 837: 1
[44] Zhao Q, Li X F, Shao Y, et al. Anal Chem, 2008, 80(19): 7586
[45] Stadtherr K, Wolf H, Lindner P. Anal Chem, 2005, 77(11): 3437
[46] Chung W J, Kim M S, Cho S, et al. Electrophoresis, 2005, 26(3): 694
[47] Xiao P, Lü X, Wang S, et al. Anal Biochem, 2013, 441(2): 123
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[49] Zheng Z, Ye M, Zuo Z, et al. Biochem J, 2006, 395(3): 509