多指标正交试验优选煎膏剂的水提工艺
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摘要
目的:制定一个优良规范的中药膏方提取工艺。方法:以黄芪肉桂四物汤煎膏剂为代表方,以出膏率和芍药苷转移率为指标,浸泡温度、浸泡时间、加水量、煎煮时间、煎煮次数为影响因素,采用正交实验法L18(37),多指标综合评分评价代表膏方的最佳提取工艺。提取工艺为从中药饮片至清膏的过程,包括浸泡、煎煮、浓缩。结果:有显著影响的因素依次是煎煮次数>加水量>煎煮时间,最佳提取工艺的有效参数为:12倍水量浸泡60min,浸泡温度30℃,提取3次,每次煎煮90 min。结论:优选的提取工艺稳定可行,可为制备其他膏方和进一步应用提供参考。
Objective: To develop an optimized and standardized extraction process for preparing herbal paste. Method:With the yield rate of paste and paeoniflorin extraction rate selected as investigational indexes,the extraction process represented by Huangqi Rougui Siwu Decoction decocted extract formula was designed by orthogonal design L18( 37) with multi-index comprehensive method to investigate the effects of influencing factors as soaking temperature,soaking time,adding water volume,decocting time and decocting number on the optimization of extraction process for the representative paste. Extraction process lasted from Chinese medicine decoction pieces to paste process,including soaking,decoction and concentration. Result: The most influencing factor for the decocted extracts yield was decocting number,followed by adding water volume and decocting time. The effective parameters for optimum extraction process was designed as follows: 60 min of soaking time,30℃ of soaking temperature,extraction for three times and 180 min each time. Conclusion: The stability and feasibility of the optimized process provide the direction for furthering application and paste preparation of other decocted agent.
Objective: To develop an optimized and standardized extraction process for preparing herbal paste. Method:With the yield rate of paste and paeoniflorin extraction rate selected as investigational indexes,the extraction process represented by Huangqi Rougui Siwu Decoction decocted extract formula was designed by orthogonal design L18( 37) with multi-index comprehensive method to investigate the effects of influencing factors as soaking temperature,soaking time,adding water volume,decocting time and decocting number on the optimization of extraction process for the representative paste. Extraction process lasted from Chinese medicine decoction pieces to paste process,including soaking,decoction and concentration. Result: The most influencing factor for the decocted extracts yield was decocting number,followed by adding water volume and decocting time. The effective parameters for optimum extraction process was designed as follows: 60 min of soaking time,30℃ of soaking temperature,extraction for three times and 180 min each time. Conclusion: The stability and feasibility of the optimized process provide the direction for furthering application and paste preparation of other decocted agent.
引文
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[12]Sue Siang,Edward John Birch.Effect of ultrasonic treatment on the polyphenol content and antioxidant capacity of extract from defatted hemp,flax and canola seed cakes[J].Ultrasonics Sonochemistry,2014,21:346-353.
[2]许天申,郭长河.四物汤及其衍生方在妇科的应用[J].河南中医,2009,29(6):605-606.
[3]杨勇,吴敏,王初.正交试验优选十全大补膏的提取工艺研究[J].中草药,2010,41(1):71-72
[4]许树军,邹淑君,王冬梅,等.桂苓五味甘草汤煎膏剂提取工艺研究[J].中医药学报,2012,40(3):115-117.
[5]马志庆,周介南.医药数理统计[M].北京:科学出版社,2012:214.
[6]李群,王瑾,张会敏.正交试验法优选桑白皮蜜炙工艺[J].中草药,2013,44(3):286-290.
[7]鲍晓东,赵金平,连胜利.正交试验法分析平帕汤治疗帕金森病的最佳配伍比例[J].中医杂志,2013,54(20):1774-1777.
[8]贾晓斌,黄洋,陈斌,等.药效学结合正交试验优选通脉颗粒抗心肌缺血成分的提取工艺研究[J].中国中药杂志,2011,36(2):154-157.
[9]Hui Zhou,Junling Liang,Da Lva,et al.Characterization of phenolics of Sarcandra glabra by non-targeted high-performance liquid chromatography fingerprinting and following targeted electrospray ionisation tandem mass spectrometry/time-of-flight mass spectrometry analyses[J].Food Chemistry,2013,138:2390–2398.
[10]张家成,章军,刘峰.加水量与煎煮时间对葛根芩连汤主要成分溶出量的影响[J].中国实验方剂学杂志,2013,19(1):13-17.
[11]解江纯,刘志东,田慧,等.芍药苷稳定性研究[J].天津中医药,2010,27(4):344-345.
[12]Sue Siang,Edward John Birch.Effect of ultrasonic treatment on the polyphenol content and antioxidant capacity of extract from defatted hemp,flax and canola seed cakes[J].Ultrasonics Sonochemistry,2014,21:346-353.